Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice
Abstract Background Bisphenol S (BPS) is increasingly used as a replacement for bisphenol A in the manufacture of products containing polycarbonates and epoxy resins. However, further studies of BPS exposure are needed for the assessment of health risks to humans. In this study we assessed the poten...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-05-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12958-020-00596-x |
| _version_ | 1819178525674962944 |
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| author | Hedvika Řimnáčová Miriam Štiavnická Jiří Moravec Marouane Chemek Yaroslav Kolinko Olga García-Álvarez Peter R. Mouton Azalia Mariel Carranza Trejo Tereza Fenclová Nikola Eretová Petr Hošek Pavel Klein Milena Králíčková Jaroslav Petr Jan Nevoral |
| author_facet | Hedvika Řimnáčová Miriam Štiavnická Jiří Moravec Marouane Chemek Yaroslav Kolinko Olga García-Álvarez Peter R. Mouton Azalia Mariel Carranza Trejo Tereza Fenclová Nikola Eretová Petr Hošek Pavel Klein Milena Králíčková Jaroslav Petr Jan Nevoral |
| author_sort | Hedvika Řimnáčová |
| collection | DOAJ |
| description | Abstract Background Bisphenol S (BPS) is increasingly used as a replacement for bisphenol A in the manufacture of products containing polycarbonates and epoxy resins. However, further studies of BPS exposure are needed for the assessment of health risks to humans. In this study we assessed the potential harmfulness of low-dose BPS on reproduction in male mice. Methods To simulate human exposure under experimental conditions, 8-week-old outbred ICR male mice received 8 weeks of drinking water containing a broad range of BPS doses [0.001, 1.0, or 100 μg/kg body weight (bw)/day, BPS1–3] or vehicle control. Mice were sacrificed and testicular tissue taken for histological analysis and protein identification by nano-liquid chromatography/mass spectrometry (MS) and sperm collected for immunodetection of acetylated lysine and phosphorylated tyrosine followed by protein characterisation using matrix-assisted laser desorption ionisation time-of-flight MS (MALDI-TOF MS). Results The results indicate that compared to vehicle, 100 μg/kg/day exposure (BPS3) leads to 1) significant histopathology in testicular tissue; and, 2) higher levels of the histone protein γH2AX, a reliable marker of DNA damage. There were fewer mature spermatozoa in the germ layer in the experimental group treated with 1 μg/kg bw (BPS2). Finally, western blot and MALDI-TOF MS studies showed significant alterations in the sperm acetylome and phosphorylome in mice treated with the lowest exposure (0.001 μg/kg/day; BPS1), although the dose is several times lower than what has been published so far. Conclusions In summary, this range of qualitative and quantitative findings in young male mice raise the possibility that very low doses of BPS may impair mammalian reproduction through epigenetic modifications of sperm proteins. |
| first_indexed | 2024-12-22T21:43:56Z |
| format | Article |
| id | doaj.art-6383fe46f85d484badecea706be1fbb3 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-12-22T21:43:56Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-6383fe46f85d484badecea706be1fbb32022-12-21T18:11:33ZengBMCReproductive Biology and Endocrinology1477-78272020-05-0118111010.1186/s12958-020-00596-xLow doses of Bisphenol S affect post-translational modifications of sperm proteins in male miceHedvika Řimnáčová0Miriam Štiavnická1Jiří Moravec2Marouane Chemek3Yaroslav Kolinko4Olga García-Álvarez5Peter R. Mouton6Azalia Mariel Carranza Trejo7Tereza Fenclová8Nikola Eretová9Petr Hošek10Pavel Klein11Milena Králíčková12Jaroslav Petr13Jan Nevoral14Biomedical Center in Pilsen, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center in Pilsen, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center in Pilsen, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center in Pilsen, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center in Pilsen, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center in Pilsen, Faculty of Medicine in Pilsen, Charles UniversitySRC Biosciences & University of South FloridaBiomedical Center in Pilsen, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center in Pilsen, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center in Pilsen, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center in Pilsen, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center in Pilsen, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center in Pilsen, Faculty of Medicine in Pilsen, Charles UniversityInstitute of Animal ScienceBiomedical Center in Pilsen, Faculty of Medicine in Pilsen, Charles UniversityAbstract Background Bisphenol S (BPS) is increasingly used as a replacement for bisphenol A in the manufacture of products containing polycarbonates and epoxy resins. However, further studies of BPS exposure are needed for the assessment of health risks to humans. In this study we assessed the potential harmfulness of low-dose BPS on reproduction in male mice. Methods To simulate human exposure under experimental conditions, 8-week-old outbred ICR male mice received 8 weeks of drinking water containing a broad range of BPS doses [0.001, 1.0, or 100 μg/kg body weight (bw)/day, BPS1–3] or vehicle control. Mice were sacrificed and testicular tissue taken for histological analysis and protein identification by nano-liquid chromatography/mass spectrometry (MS) and sperm collected for immunodetection of acetylated lysine and phosphorylated tyrosine followed by protein characterisation using matrix-assisted laser desorption ionisation time-of-flight MS (MALDI-TOF MS). Results The results indicate that compared to vehicle, 100 μg/kg/day exposure (BPS3) leads to 1) significant histopathology in testicular tissue; and, 2) higher levels of the histone protein γH2AX, a reliable marker of DNA damage. There were fewer mature spermatozoa in the germ layer in the experimental group treated with 1 μg/kg bw (BPS2). Finally, western blot and MALDI-TOF MS studies showed significant alterations in the sperm acetylome and phosphorylome in mice treated with the lowest exposure (0.001 μg/kg/day; BPS1), although the dose is several times lower than what has been published so far. Conclusions In summary, this range of qualitative and quantitative findings in young male mice raise the possibility that very low doses of BPS may impair mammalian reproduction through epigenetic modifications of sperm proteins.http://link.springer.com/article/10.1186/s12958-020-00596-xMale reproductionEndocrine disruptorLow dose effectBisphenol SPost-translational modification |
| spellingShingle | Hedvika Řimnáčová Miriam Štiavnická Jiří Moravec Marouane Chemek Yaroslav Kolinko Olga García-Álvarez Peter R. Mouton Azalia Mariel Carranza Trejo Tereza Fenclová Nikola Eretová Petr Hošek Pavel Klein Milena Králíčková Jaroslav Petr Jan Nevoral Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice Reproductive Biology and Endocrinology Male reproduction Endocrine disruptor Low dose effect Bisphenol S Post-translational modification |
| title | Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice |
| title_full | Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice |
| title_fullStr | Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice |
| title_full_unstemmed | Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice |
| title_short | Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice |
| title_sort | low doses of bisphenol s affect post translational modifications of sperm proteins in male mice |
| topic | Male reproduction Endocrine disruptor Low dose effect Bisphenol S Post-translational modification |
| url | http://link.springer.com/article/10.1186/s12958-020-00596-x |
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