Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 genome editing
Holt-Oram syndrome (HOS), which is caused by genetic changes in the TBX5 gene, affects the hands and heart. HOS patients have heart defects, including atrial septal defects (ASD), ventricular septal defects (VSD) and heart conduction disease. Here, we generated a homozygous TBX5 knockout human embry...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-03-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506121000027 |
| _version_ | 1818430653965795328 |
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| author | Tiantian Zhao Rui Bai Fujian Wu Wen-Jing Lu Jun Zhang |
| author_facet | Tiantian Zhao Rui Bai Fujian Wu Wen-Jing Lu Jun Zhang |
| author_sort | Tiantian Zhao |
| collection | DOAJ |
| description | Holt-Oram syndrome (HOS), which is caused by genetic changes in the TBX5 gene, affects the hands and heart. HOS patients have heart defects, including atrial septal defects (ASD), ventricular septal defects (VSD) and heart conduction disease. Here, we generated a homozygous TBX5 knockout human embryonic stem cell (hESC) line (TBX5-KO) using a CRISPR/Cas9 system. The TBX5-KO maintained stem cell like morphology, pluripotency markers, normal karyotype, and could differentiate into all three germ layers in vivo. This cell line can provide an in vitro platform for studying the pathogenic mechanisms and biological function of TBX5 in the heart development. |
| first_indexed | 2024-12-14T15:36:50Z |
| format | Article |
| id | doaj.art-6388bf02e892435ca8a467cd573ca4b3 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-14T15:36:50Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-6388bf02e892435ca8a467cd573ca4b32022-12-21T22:55:43ZengElsevierStem Cell Research1873-50612021-03-0151102156Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 genome editingTiantian Zhao0Rui Bai1Fujian Wu2Wen-Jing Lu3Jun Zhang4Anzhen Hospital, Capital Medical University, Beijing 100029, ChinaAnzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, ChinaTranslational Medicine Collaborative Innovation Center, The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen 518020, China; Post-doctoral Scientific Research Station of Basic Medicine, Jinan University, Guangzhou 510632, ChinaAnzhen Hospital, Capital Medical University, Beijing 100029, ChinaAnzhen Hospital, Capital Medical University, Beijing 100029, China; Corresponding author at: Beijing Anzhen Hospital, 2 Anzhen Road, Chaoyang District, Beijing 100029, China.Holt-Oram syndrome (HOS), which is caused by genetic changes in the TBX5 gene, affects the hands and heart. HOS patients have heart defects, including atrial septal defects (ASD), ventricular septal defects (VSD) and heart conduction disease. Here, we generated a homozygous TBX5 knockout human embryonic stem cell (hESC) line (TBX5-KO) using a CRISPR/Cas9 system. The TBX5-KO maintained stem cell like morphology, pluripotency markers, normal karyotype, and could differentiate into all three germ layers in vivo. This cell line can provide an in vitro platform for studying the pathogenic mechanisms and biological function of TBX5 in the heart development.http://www.sciencedirect.com/science/article/pii/S1873506121000027 |
| spellingShingle | Tiantian Zhao Rui Bai Fujian Wu Wen-Jing Lu Jun Zhang Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 genome editing Stem Cell Research |
| title | Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 genome editing |
| title_full | Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 genome editing |
| title_fullStr | Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 genome editing |
| title_full_unstemmed | Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 genome editing |
| title_short | Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 genome editing |
| title_sort | generation of a tbx5 homozygous knockout embryonic stem cell line wae009 a 45 by crispr cas9 genome editing |
| url | http://www.sciencedirect.com/science/article/pii/S1873506121000027 |
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