Raman Spectroscopy-Based Quality Control of “Silicon-On-Insulator” Nanowire Chips for the Detection of Brain Cancer-Associated MicroRNA in Plasma
Application of micro-Raman spectroscopy for the monitoring of quality of nanowire sensor chips fabrication has been demonstrated. Nanowire chips have been fabricated on the basis of «silicon-on-insulator» (SOI) structures (SOI-NW chips). The fabrication of SOI-NW chips was performed by optical litog...
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2021-02-01
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author | Kristina A. Malsagova Vladimir P. Popov Igor N. Kupriyanov Tatyana O. Pleshakova Rafael A. Galiullin Andrey F. Kozlov Ivan D. Shumov Dmitry I. Larionov Fedor V. Tikhonenko Svetlana I. Kapustina Vadim S. Ziborov Oleg F. Petrov Olga A. Gadzhieva Boris A. Bashiryan Vadim N. Shimansky Alexander I. Archakov Yuri D. Ivanov |
author_facet | Kristina A. Malsagova Vladimir P. Popov Igor N. Kupriyanov Tatyana O. Pleshakova Rafael A. Galiullin Andrey F. Kozlov Ivan D. Shumov Dmitry I. Larionov Fedor V. Tikhonenko Svetlana I. Kapustina Vadim S. Ziborov Oleg F. Petrov Olga A. Gadzhieva Boris A. Bashiryan Vadim N. Shimansky Alexander I. Archakov Yuri D. Ivanov |
author_sort | Kristina A. Malsagova |
collection | DOAJ |
description | Application of micro-Raman spectroscopy for the monitoring of quality of nanowire sensor chips fabrication has been demonstrated. Nanowire chips have been fabricated on the basis of «silicon-on-insulator» (SOI) structures (SOI-NW chips). The fabrication of SOI-NW chips was performed by optical litography with gas-phase etching. The so-fabricated SOI-NW chips are intended for highly sensitive detection of brain cancer biomarkers in humans. In our present study, two series of experiments have been conducted. In the first experimental series, detection of a synthetic DNA oligonucleotide (oDNA) analogue of brain cancer-associated microRNA miRNA-363 in purified buffer solution has been performed in order to demonstrate the high detection sensitivity. The second experimental series has been performed in order to reveal miRNA-363 itself in real human plasma samples. To provide detection biospecificity, the SOI-NW chip surface was modified by covalent immobilization of probe oligonucleotides (oDNA probes) complementary to the target biomolecules. Using the SOI-NW sensor chips proposed herein, the concentration detection limit of the target biomolecules at the level of 3.3 × 10<sup>−17</sup> M has been demonstrated. Thus, the approach employing the SOI-NW chips proposed herein represents an attractive tool in biomedical practice, aimed at the early revelation of oncological diseases in humans. |
first_indexed | 2024-03-09T00:54:27Z |
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issn | 1424-8220 |
language | English |
last_indexed | 2024-03-09T00:54:27Z |
publishDate | 2021-02-01 |
publisher | MDPI AG |
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series | Sensors |
spelling | doaj.art-638946575f0746de9f7716510baef0052023-12-11T17:00:06ZengMDPI AGSensors1424-82202021-02-01214133310.3390/s21041333Raman Spectroscopy-Based Quality Control of “Silicon-On-Insulator” Nanowire Chips for the Detection of Brain Cancer-Associated MicroRNA in PlasmaKristina A. Malsagova0Vladimir P. Popov1Igor N. Kupriyanov2Tatyana O. Pleshakova3Rafael A. Galiullin4Andrey F. Kozlov5Ivan D. Shumov6Dmitry I. Larionov7Fedor V. Tikhonenko8Svetlana I. Kapustina9Vadim S. Ziborov10Oleg F. Petrov11Olga A. Gadzhieva12Boris A. Bashiryan13Vadim N. Shimansky14Alexander I. Archakov15Yuri D. Ivanov16Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaRzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaSobolev Institute of Geology and Mineralogy, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaLaboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaLaboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaLaboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaLaboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaLaboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaRzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaLaboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaLaboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaJoint Institute for High Temperatures of Russian Academy of Sciences, 125412 Moscow, RussiaFederal State Autonomous Institution “N. N. Burdenko National Medical Research Center of Neurosurgery” of the Ministry of Health of the Russian Federation, 125047 Moscow, RussiaFederal State Autonomous Institution “N. N. Burdenko National Medical Research Center of Neurosurgery” of the Ministry of Health of the Russian Federation, 125047 Moscow, RussiaFederal State Autonomous Institution “N. N. Burdenko National Medical Research Center of Neurosurgery” of the Ministry of Health of the Russian Federation, 125047 Moscow, RussiaLaboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaLaboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaApplication of micro-Raman spectroscopy for the monitoring of quality of nanowire sensor chips fabrication has been demonstrated. Nanowire chips have been fabricated on the basis of «silicon-on-insulator» (SOI) structures (SOI-NW chips). The fabrication of SOI-NW chips was performed by optical litography with gas-phase etching. The so-fabricated SOI-NW chips are intended for highly sensitive detection of brain cancer biomarkers in humans. In our present study, two series of experiments have been conducted. In the first experimental series, detection of a synthetic DNA oligonucleotide (oDNA) analogue of brain cancer-associated microRNA miRNA-363 in purified buffer solution has been performed in order to demonstrate the high detection sensitivity. The second experimental series has been performed in order to reveal miRNA-363 itself in real human plasma samples. To provide detection biospecificity, the SOI-NW chip surface was modified by covalent immobilization of probe oligonucleotides (oDNA probes) complementary to the target biomolecules. Using the SOI-NW sensor chips proposed herein, the concentration detection limit of the target biomolecules at the level of 3.3 × 10<sup>−17</sup> M has been demonstrated. Thus, the approach employing the SOI-NW chips proposed herein represents an attractive tool in biomedical practice, aimed at the early revelation of oncological diseases in humans.https://www.mdpi.com/1424-8220/21/4/1333nanowire biosensorsensor chipsilicon-on-insulatorbrain cancerdiagnosticsRaman spectroscopy |
spellingShingle | Kristina A. Malsagova Vladimir P. Popov Igor N. Kupriyanov Tatyana O. Pleshakova Rafael A. Galiullin Andrey F. Kozlov Ivan D. Shumov Dmitry I. Larionov Fedor V. Tikhonenko Svetlana I. Kapustina Vadim S. Ziborov Oleg F. Petrov Olga A. Gadzhieva Boris A. Bashiryan Vadim N. Shimansky Alexander I. Archakov Yuri D. Ivanov Raman Spectroscopy-Based Quality Control of “Silicon-On-Insulator” Nanowire Chips for the Detection of Brain Cancer-Associated MicroRNA in Plasma Sensors nanowire biosensor sensor chip silicon-on-insulator brain cancer diagnostics Raman spectroscopy |
title | Raman Spectroscopy-Based Quality Control of “Silicon-On-Insulator” Nanowire Chips for the Detection of Brain Cancer-Associated MicroRNA in Plasma |
title_full | Raman Spectroscopy-Based Quality Control of “Silicon-On-Insulator” Nanowire Chips for the Detection of Brain Cancer-Associated MicroRNA in Plasma |
title_fullStr | Raman Spectroscopy-Based Quality Control of “Silicon-On-Insulator” Nanowire Chips for the Detection of Brain Cancer-Associated MicroRNA in Plasma |
title_full_unstemmed | Raman Spectroscopy-Based Quality Control of “Silicon-On-Insulator” Nanowire Chips for the Detection of Brain Cancer-Associated MicroRNA in Plasma |
title_short | Raman Spectroscopy-Based Quality Control of “Silicon-On-Insulator” Nanowire Chips for the Detection of Brain Cancer-Associated MicroRNA in Plasma |
title_sort | raman spectroscopy based quality control of silicon on insulator nanowire chips for the detection of brain cancer associated microrna in plasma |
topic | nanowire biosensor sensor chip silicon-on-insulator brain cancer diagnostics Raman spectroscopy |
url | https://www.mdpi.com/1424-8220/21/4/1333 |
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