АNTIVIRAL ACTIVITY OF LIPOPOLYSACCHARIDES OF Pseudomonas chlororaphis subsp. aureofaciens
Lipopolysaccharides and their fractions were obtained from strains Pseudomonas chlororaphis subsp. aureofaciens UCM В-111 and UCM В-306 — components of insectofungicidal gaupsin biopreparation; their activity against tobacco mosaic virus has been studied. Lipopolysaccharides of strains В-111 and В-3...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2013-04-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/no2_2013/kiprianova_2_2013.pdf |
| _version_ | 1797766786716794880 |
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| author | Е. А. Kiprianova L. D. Varbanets V. V. Shepelevich S. I. Voichuk |
| author_facet | Е. А. Kiprianova L. D. Varbanets V. V. Shepelevich S. I. Voichuk |
| author_sort | Е. А. Kiprianova |
| collection | DOAJ |
| description | Lipopolysaccharides and their fractions were obtained from strains Pseudomonas chlororaphis subsp. aureofaciens UCM В-111 and UCM В-306 — components of insectofungicidal gaupsin biopreparation; their activity against tobacco mosaic virus has been studied. Lipopolysaccharides of strains В-111 and В-306 proved to be highly active antiviral agents and inhibited tobacco mosaic virus infectivity for three species of indicating plants in concentrations 0,001–10 mg/ml. First lipopolysaccharides fraction (O-specific side chains) did not inhibit and often stimulated the virus reproduction. At the same time the core oligosaccharides (the second and the third lipopolysaccharides fractions) decreased to different extent the virus infection development. According to electron microscopy data the virions sticked together forming the sneafs at the direct lipopolysaccharides-virus contact in vitro whereas the single free virus particles were observed in the control. Evidence of direct interaction between lipopolysaccharides of P. chlororaphis subsp. aureofaciens strains and tobacco mosaic virus is provided. |
| first_indexed | 2024-03-12T20:30:14Z |
| format | Article |
| id | doaj.art-638b982085bf44fab5a2928113714c00 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T20:30:14Z |
| publishDate | 2013-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-638b982085bf44fab5a2928113714c002023-08-02T00:07:32ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2013-04-0162687310.15407/biotech6.02.068АNTIVIRAL ACTIVITY OF LIPOPOLYSACCHARIDES OF Pseudomonas chlororaphis subsp. aureofaciensЕ. А. Kiprianova0 L. D. Varbanets1V. V. Shepelevich2 S. I. Voichuk3Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, KyivZabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, KyivTaras Shevchenko Kyiv National UniversityZabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, KyivLipopolysaccharides and their fractions were obtained from strains Pseudomonas chlororaphis subsp. aureofaciens UCM В-111 and UCM В-306 — components of insectofungicidal gaupsin biopreparation; their activity against tobacco mosaic virus has been studied. Lipopolysaccharides of strains В-111 and В-306 proved to be highly active antiviral agents and inhibited tobacco mosaic virus infectivity for three species of indicating plants in concentrations 0,001–10 mg/ml. First lipopolysaccharides fraction (O-specific side chains) did not inhibit and often stimulated the virus reproduction. At the same time the core oligosaccharides (the second and the third lipopolysaccharides fractions) decreased to different extent the virus infection development. According to electron microscopy data the virions sticked together forming the sneafs at the direct lipopolysaccharides-virus contact in vitro whereas the single free virus particles were observed in the control. Evidence of direct interaction between lipopolysaccharides of P. chlororaphis subsp. aureofaciens strains and tobacco mosaic virus is provided.http://biotechnology.kiev.ua/images/storage/no2_2013/kiprianova_2_2013.pdfPseudomonas chlororaphis subsp. aureofaciens lipopolysaccharidestobacco mosaic virusinfectivity inhibitionelectron microscopy |
| spellingShingle | Е. А. Kiprianova L. D. Varbanets V. V. Shepelevich S. I. Voichuk АNTIVIRAL ACTIVITY OF LIPOPOLYSACCHARIDES OF Pseudomonas chlororaphis subsp. aureofaciens Biotechnologia Acta Pseudomonas chlororaphis subsp. aureofaciens lipopolysaccharides tobacco mosaic virus infectivity inhibition electron microscopy |
| title | АNTIVIRAL ACTIVITY OF LIPOPOLYSACCHARIDES OF Pseudomonas chlororaphis subsp. aureofaciens |
| title_full | АNTIVIRAL ACTIVITY OF LIPOPOLYSACCHARIDES OF Pseudomonas chlororaphis subsp. aureofaciens |
| title_fullStr | АNTIVIRAL ACTIVITY OF LIPOPOLYSACCHARIDES OF Pseudomonas chlororaphis subsp. aureofaciens |
| title_full_unstemmed | АNTIVIRAL ACTIVITY OF LIPOPOLYSACCHARIDES OF Pseudomonas chlororaphis subsp. aureofaciens |
| title_short | АNTIVIRAL ACTIVITY OF LIPOPOLYSACCHARIDES OF Pseudomonas chlororaphis subsp. aureofaciens |
| title_sort | аntiviral activity of lipopolysaccharides of pseudomonas chlororaphis subsp aureofaciens |
| topic | Pseudomonas chlororaphis subsp. aureofaciens lipopolysaccharides tobacco mosaic virus infectivity inhibition electron microscopy |
| url | http://biotechnology.kiev.ua/images/storage/no2_2013/kiprianova_2_2013.pdf |
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