Accelerating the Biodegradation of High-Density Polyethylene (HDPE) Using <i>Bjerkandera adusta</i> TBB-03 and Lignocellulose Substrates
High-density polyethylene (HDPE) is a widely used organic polymer and an emerging pollutant, because it is very stable and nonbiodegradable. Several fungal species that produce delignifying enzymes are known to be promising degraders of recalcitrant polymers, but research on the decomposition of pla...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/9/304 |
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| author | Bo Ram Kang Soo Bin Kim Hyun A Song Tae Kwon Lee |
| author_facet | Bo Ram Kang Soo Bin Kim Hyun A Song Tae Kwon Lee |
| author_sort | Bo Ram Kang |
| collection | DOAJ |
| description | High-density polyethylene (HDPE) is a widely used organic polymer and an emerging pollutant, because it is very stable and nonbiodegradable. Several fungal species that produce delignifying enzymes are known to be promising degraders of recalcitrant polymers, but research on the decomposition of plastics is scarce. In this study, white rot fungus, <i>Bjerkandera adusta</i> TBB-03, was isolated and characterized for its ability to degrade HDPE under lignocellulose substrate treatment. Ash (<i>Fraxinus rhynchophylla</i>) wood chips were found to stimulate laccase production (activity was > 210 U/L after 10 days of cultivation), and subsequently used for HDPE degradation assay. After 90 days, cracks formed on the surface of HDPE samples treated with TBB-03 and ash wood chips in both liquid and solid states. Raman analysis showed that the amorphous structure of HDPE was degraded by enzymes produced by TBB-03. Overall, TBB-03 is a promising resource for the biodegradation of HDPE, and this work sheds light on further applications for fungus-based plastic degradation systems. |
| first_indexed | 2024-12-11T02:54:48Z |
| format | Article |
| id | doaj.art-638df77a9a2f47b199dbbc97b76fc5a8 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-11T02:54:48Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-638df77a9a2f47b199dbbc97b76fc5a82022-12-22T01:23:12ZengMDPI AGMicroorganisms2076-26072019-08-017930410.3390/microorganisms7090304microorganisms7090304Accelerating the Biodegradation of High-Density Polyethylene (HDPE) Using <i>Bjerkandera adusta</i> TBB-03 and Lignocellulose SubstratesBo Ram Kang0Soo Bin Kim1Hyun A Song2Tae Kwon Lee3Department of Environmental Engineering, Yonsei University, Wonju 26493, KoreaDepartment of Environmental Engineering, Yonsei University, Wonju 26493, KoreaDepartment of Environmental Engineering, Yonsei University, Wonju 26493, KoreaDepartment of Environmental Engineering, Yonsei University, Wonju 26493, KoreaHigh-density polyethylene (HDPE) is a widely used organic polymer and an emerging pollutant, because it is very stable and nonbiodegradable. Several fungal species that produce delignifying enzymes are known to be promising degraders of recalcitrant polymers, but research on the decomposition of plastics is scarce. In this study, white rot fungus, <i>Bjerkandera adusta</i> TBB-03, was isolated and characterized for its ability to degrade HDPE under lignocellulose substrate treatment. Ash (<i>Fraxinus rhynchophylla</i>) wood chips were found to stimulate laccase production (activity was > 210 U/L after 10 days of cultivation), and subsequently used for HDPE degradation assay. After 90 days, cracks formed on the surface of HDPE samples treated with TBB-03 and ash wood chips in both liquid and solid states. Raman analysis showed that the amorphous structure of HDPE was degraded by enzymes produced by TBB-03. Overall, TBB-03 is a promising resource for the biodegradation of HDPE, and this work sheds light on further applications for fungus-based plastic degradation systems.https://www.mdpi.com/2076-2607/7/9/304high-density polyethylene (HDPE)<i>Bjerkandera adusta</i>laccaseRaman spectroscopyScanning electron microscopy |
| spellingShingle | Bo Ram Kang Soo Bin Kim Hyun A Song Tae Kwon Lee Accelerating the Biodegradation of High-Density Polyethylene (HDPE) Using <i>Bjerkandera adusta</i> TBB-03 and Lignocellulose Substrates Microorganisms high-density polyethylene (HDPE) <i>Bjerkandera adusta</i> laccase Raman spectroscopy Scanning electron microscopy |
| title | Accelerating the Biodegradation of High-Density Polyethylene (HDPE) Using <i>Bjerkandera adusta</i> TBB-03 and Lignocellulose Substrates |
| title_full | Accelerating the Biodegradation of High-Density Polyethylene (HDPE) Using <i>Bjerkandera adusta</i> TBB-03 and Lignocellulose Substrates |
| title_fullStr | Accelerating the Biodegradation of High-Density Polyethylene (HDPE) Using <i>Bjerkandera adusta</i> TBB-03 and Lignocellulose Substrates |
| title_full_unstemmed | Accelerating the Biodegradation of High-Density Polyethylene (HDPE) Using <i>Bjerkandera adusta</i> TBB-03 and Lignocellulose Substrates |
| title_short | Accelerating the Biodegradation of High-Density Polyethylene (HDPE) Using <i>Bjerkandera adusta</i> TBB-03 and Lignocellulose Substrates |
| title_sort | accelerating the biodegradation of high density polyethylene hdpe using i bjerkandera adusta i tbb 03 and lignocellulose substrates |
| topic | high-density polyethylene (HDPE) <i>Bjerkandera adusta</i> laccase Raman spectroscopy Scanning electron microscopy |
| url | https://www.mdpi.com/2076-2607/7/9/304 |
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