(Pro)renin Receptor Is Present in Human Sperm and It Adversely Affects Sperm Fertility Ability
Sperm fertility ability may be modulated by different molecular systems, such as the renin-angiotensin system (RAS). Although renin is one of its most relevant peptides, the presence and role of the (pro)renin receptor (PRR) is completely unknown. We have proved for the first time the existence of P...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/6/3215 |
| _version_ | 1797540490580590592 |
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| author | Marta Gianzo Itziar Urizar-Arenaza Iraia Muñoa-Hoyos Zaloa Larreategui Nicolás Garrido Jon Irazusta Nerea Subirán |
| author_facet | Marta Gianzo Itziar Urizar-Arenaza Iraia Muñoa-Hoyos Zaloa Larreategui Nicolás Garrido Jon Irazusta Nerea Subirán |
| author_sort | Marta Gianzo |
| collection | DOAJ |
| description | Sperm fertility ability may be modulated by different molecular systems, such as the renin-angiotensin system (RAS). Although renin is one of its most relevant peptides, the presence and role of the (pro)renin receptor (PRR) is completely unknown. We have proved for the first time the existence of PRR and its transcript in human sperm by western blot and RT-PCR. Immunofluorescence studies showed that this receptor is mainly located in the apical region over the acrosome and in the postacrosomal region of the sperm head and along the sperm tail. In addition, this prospective cohort study also proves that semen samples with higher percentages of PRR-positive spermatozoa are associated with poor sperm motility, worse blastocyst development and no-viable blastocysts. Our results provide insight into how PRR play a negative role in sperm physiology that it may condition human embryo quality and development. An in-depth understanding of the role of PRR in sperm fertility can help elucidate its role in male infertility, as well as establish biomarkers for the diagnosis or selection of sperm to use during assisted reproductive techniques. |
| first_indexed | 2024-03-10T13:00:57Z |
| format | Article |
| id | doaj.art-6391405c2d35445daed68159b88b3f5d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T13:00:57Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6391405c2d35445daed68159b88b3f5d2023-11-21T11:29:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01226321510.3390/ijms22063215(Pro)renin Receptor Is Present in Human Sperm and It Adversely Affects Sperm Fertility AbilityMarta Gianzo0Itziar Urizar-Arenaza1Iraia Muñoa-Hoyos2Zaloa Larreategui3Nicolás Garrido4Jon Irazusta5Nerea Subirán6Department of Physiology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), 48940 Bizkaia, SpainDepartment of Physiology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), 48940 Bizkaia, SpainDepartment of Physiology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), 48940 Bizkaia, SpainValencian Institute of Infertility (IVI-RMA)-Bilbao, 48940 Bizkaia, SpainIVI Foundation, Instituto de Investigación Sanitaria La Fe (IIS La Fe), 46015 Valencia, SpainDepartment of Physiology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), 48940 Bizkaia, SpainDepartment of Physiology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), 48940 Bizkaia, SpainSperm fertility ability may be modulated by different molecular systems, such as the renin-angiotensin system (RAS). Although renin is one of its most relevant peptides, the presence and role of the (pro)renin receptor (PRR) is completely unknown. We have proved for the first time the existence of PRR and its transcript in human sperm by western blot and RT-PCR. Immunofluorescence studies showed that this receptor is mainly located in the apical region over the acrosome and in the postacrosomal region of the sperm head and along the sperm tail. In addition, this prospective cohort study also proves that semen samples with higher percentages of PRR-positive spermatozoa are associated with poor sperm motility, worse blastocyst development and no-viable blastocysts. Our results provide insight into how PRR play a negative role in sperm physiology that it may condition human embryo quality and development. An in-depth understanding of the role of PRR in sperm fertility can help elucidate its role in male infertility, as well as establish biomarkers for the diagnosis or selection of sperm to use during assisted reproductive techniques.https://www.mdpi.com/1422-0067/22/6/3215prorenin receptorhumansemen analysisreproductive techniquesassistedembryonic development |
| spellingShingle | Marta Gianzo Itziar Urizar-Arenaza Iraia Muñoa-Hoyos Zaloa Larreategui Nicolás Garrido Jon Irazusta Nerea Subirán (Pro)renin Receptor Is Present in Human Sperm and It Adversely Affects Sperm Fertility Ability International Journal of Molecular Sciences prorenin receptor human semen analysis reproductive techniques assisted embryonic development |
| title | (Pro)renin Receptor Is Present in Human Sperm and It Adversely Affects Sperm Fertility Ability |
| title_full | (Pro)renin Receptor Is Present in Human Sperm and It Adversely Affects Sperm Fertility Ability |
| title_fullStr | (Pro)renin Receptor Is Present in Human Sperm and It Adversely Affects Sperm Fertility Ability |
| title_full_unstemmed | (Pro)renin Receptor Is Present in Human Sperm and It Adversely Affects Sperm Fertility Ability |
| title_short | (Pro)renin Receptor Is Present in Human Sperm and It Adversely Affects Sperm Fertility Ability |
| title_sort | pro renin receptor is present in human sperm and it adversely affects sperm fertility ability |
| topic | prorenin receptor human semen analysis reproductive techniques assisted embryonic development |
| url | https://www.mdpi.com/1422-0067/22/6/3215 |
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