Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency Genes
Induced pluripotent stem cells (iPSCs) can differentiate into all types of cells and can be used in livestock for research on biological development, genetic breeding, and in vitro genetic resource conservation. The Bactrian camel is a large domestic animal that inhabits extreme environments and hol...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/1917 |
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| author | Zongshuai Li Yina Li Qiran Zhang Wenbo Ge Yong Zhang Xingxu Zhao Junjie Hu Ligang Yuan Wangdong Zhang |
| author_facet | Zongshuai Li Yina Li Qiran Zhang Wenbo Ge Yong Zhang Xingxu Zhao Junjie Hu Ligang Yuan Wangdong Zhang |
| author_sort | Zongshuai Li |
| collection | DOAJ |
| description | Induced pluripotent stem cells (iPSCs) can differentiate into all types of cells and can be used in livestock for research on biological development, genetic breeding, and in vitro genetic resource conservation. The Bactrian camel is a large domestic animal that inhabits extreme environments and holds value in the treatment of various diseases and the development of the local economy. Therefore, we transferred four mouse genes (<i>Oct4</i>, <i>Sox2</i>, <i>Klf4</i>, and c-<i>Myc</i>) into Bactrian camel fetal fibroblasts (BCFFs) using retroviruses with a large host range to obtain Bactrian camel induced pluripotent stem cells (bciPSCs). They were comprehensively identified based on cell morphology, pluripotency gene and marker expression, chromosome number, transcriptome sequencing, and differentiation potential. The results showed the pluripotency of bciPSCs. However, unlike stem cells of other species, late formation of stem cell clones was observed; moreover, the immunofluorescence of SSEA1, SSEA3, and SSEA4 were positive, and teratoma formation took four months. These findings may be related to the extremely long gestation period and species specificity of Bactrian camels. By mining RNA sequence data, 85 potential unique pluripotent genes of Bactrian camels were predicted, which could be used as candidate genes for the production of bciPSC in the future. Among them, <i>ASF1B</i>, <i>DTL</i>, <i>CDCA5</i>, <i>PROM1</i>, <i>CYTL1</i>, <i>NUP210</i>, <i>Epha3</i>, and <i>SYT13</i> are more attractive. In conclusion, we generated bciPSCs for the first time and obtained their transcriptome information, expanding the iPSC genetic information database and exploring the applicability of iPSCs in livestock. Our results can provide an experimental basis for Bactrian camel ESC establishment, developmental research, and genetic resource conservation. |
| first_indexed | 2024-03-11T09:42:49Z |
| format | Article |
| id | doaj.art-6391d6c314344ebf8d88f60cdda2db48 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:42:49Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6391d6c314344ebf8d88f60cdda2db482023-11-16T16:49:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01243191710.3390/ijms24031917Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency GenesZongshuai Li0Yina Li1Qiran Zhang2Wenbo Ge3Yong Zhang4Xingxu Zhao5Junjie Hu6Ligang Yuan7Wangdong Zhang8College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Gansu Agricultural University, Lanzhou 730070, ChinaChinese Academy of Agricultural Sciences Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaInduced pluripotent stem cells (iPSCs) can differentiate into all types of cells and can be used in livestock for research on biological development, genetic breeding, and in vitro genetic resource conservation. The Bactrian camel is a large domestic animal that inhabits extreme environments and holds value in the treatment of various diseases and the development of the local economy. Therefore, we transferred four mouse genes (<i>Oct4</i>, <i>Sox2</i>, <i>Klf4</i>, and c-<i>Myc</i>) into Bactrian camel fetal fibroblasts (BCFFs) using retroviruses with a large host range to obtain Bactrian camel induced pluripotent stem cells (bciPSCs). They were comprehensively identified based on cell morphology, pluripotency gene and marker expression, chromosome number, transcriptome sequencing, and differentiation potential. The results showed the pluripotency of bciPSCs. However, unlike stem cells of other species, late formation of stem cell clones was observed; moreover, the immunofluorescence of SSEA1, SSEA3, and SSEA4 were positive, and teratoma formation took four months. These findings may be related to the extremely long gestation period and species specificity of Bactrian camels. By mining RNA sequence data, 85 potential unique pluripotent genes of Bactrian camels were predicted, which could be used as candidate genes for the production of bciPSC in the future. Among them, <i>ASF1B</i>, <i>DTL</i>, <i>CDCA5</i>, <i>PROM1</i>, <i>CYTL1</i>, <i>NUP210</i>, <i>Epha3</i>, and <i>SYT13</i> are more attractive. In conclusion, we generated bciPSCs for the first time and obtained their transcriptome information, expanding the iPSC genetic information database and exploring the applicability of iPSCs in livestock. Our results can provide an experimental basis for Bactrian camel ESC establishment, developmental research, and genetic resource conservation.https://www.mdpi.com/1422-0067/24/3/1917Bactrian camelinduced pluripotent stem cellsreprogrammingRNA-Seqpluripotency genes |
| spellingShingle | Zongshuai Li Yina Li Qiran Zhang Wenbo Ge Yong Zhang Xingxu Zhao Junjie Hu Ligang Yuan Wangdong Zhang Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency Genes International Journal of Molecular Sciences Bactrian camel induced pluripotent stem cells reprogramming RNA-Seq pluripotency genes |
| title | Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency Genes |
| title_full | Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency Genes |
| title_fullStr | Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency Genes |
| title_full_unstemmed | Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency Genes |
| title_short | Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency Genes |
| title_sort | establishment of bactrian camel induced pluripotent stem cells and prediction of their unique pluripotency genes |
| topic | Bactrian camel induced pluripotent stem cells reprogramming RNA-Seq pluripotency genes |
| url | https://www.mdpi.com/1422-0067/24/3/1917 |
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