Assessment of the Accuracy of High-Throughput Sequencing of the ITS1 Region of Neocallimastigomycota for Community Composition Analysis

Assessment of the Accuracy of High-Throughput Sequencing of the ITS1 Region of Neocallimastigomycota for Community Composition Analysis

Anaerobic fungi (Neocallimastigomycota) are common inhabitants of the digestive tract of large mammalian herbivores, where they make an important contribution to plant biomass degradation. The internal transcribed spacer 1 (ITS1) region is currently the molecular marker of choice for anaerobic funga...

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Bibliographic Details
Main Authors: Joan E. Edwards, Gerben D. A. Hermes, Sandra Kittelmann, Bart Nijsse, Hauke Smidt
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-10-01
Series:Frontiers in Microbiology
Subjects:
Neocallimastigomycota
anaerobic fungi
internal transcribed spacer 1 region
high-throughput sequencing
clone library
size polymorphism
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.02370/full

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https://www.frontiersin.org/article/10.3389/fmicb.2019.02370/full

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