Gene Co-expression Network and Regression Analysis Identify the Transcriptomic, Physiological, and Biochemical Indicators of the Response of Alpine Woody Plant Rhododendron rex to Drought Stress
Increasing severity of drought stress due to global change and extreme weather has been affecting the biodiversity, function, and stability of forest ecosystems. However, despite being an important component in the alpine and subalpine vegetation in forest ecosystems, Rhododendron species have been...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.896691/full |
| _version_ | 1818554695281541120 |
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| author | Xiong-Li Zhou Xiong-Li Zhou Xiong-Li Zhou Jin-Yan Ma Zhen-Dian Liu Ni-fei Dai Hui-Qin Yang Liu Yang Liu Yang Yue-Hua Wang Yue-Hua Wang Shi-Kang Shen Shi-Kang Shen Shi-Kang Shen |
| author_facet | Xiong-Li Zhou Xiong-Li Zhou Xiong-Li Zhou Jin-Yan Ma Zhen-Dian Liu Ni-fei Dai Hui-Qin Yang Liu Yang Liu Yang Yue-Hua Wang Yue-Hua Wang Shi-Kang Shen Shi-Kang Shen Shi-Kang Shen |
| author_sort | Xiong-Li Zhou |
| collection | DOAJ |
| description | Increasing severity of drought stress due to global change and extreme weather has been affecting the biodiversity, function, and stability of forest ecosystems. However, despite being an important component in the alpine and subalpine vegetation in forest ecosystems, Rhododendron species have been paid rare attention in the study of molecular mechanism of tolerance or response to drought. Herein, we investigated the correlation of transcriptomic changes with the physiological and biochemical indicators of Rhododendron rex under drought stress by using the co-expression network approach and regression analysis. Compared with the control treatment, the number of significantly differentially expressed unigenes (DEGs) increased with the degree of drought stress. The DEGs were mainly enriched in the cell wall metabolic process, signaling pathways, sugar metabolism, and nitrogen metabolism. Coupled analysis of the transcriptome, physiological, and biochemical parameters indicated that the metabolic pathways were highly correlated with the physiological and biochemical indicators under drought stress, especially the chlorophyll fluorescence parameters, such as the actual photosynthetic efficiency of photosystem II, electron transport rate, photochemical quenching coefficient, and the maximum quantum efficiency of photosystem II photochemistry. The majority of the response genes related to the metabolic pathways, including photosynthesis, sugar metabolism, and phytohormone signal pathway, were highly expressed under drought stress. In addition, genes associated with cell wall, pectin, and galacturonan metabolism also played crucial roles in the response of R. rex to drought stress. The results provided novel insight into the molecular response of the alpine woody species under drought stress and may improve the understanding of the response of forest ecosystems to the global climate change. |
| first_indexed | 2024-12-12T09:43:22Z |
| format | Article |
| id | doaj.art-6396348bfbd44491b7b37720854adf80 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T09:43:22Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-6396348bfbd44491b7b37720854adf802022-12-22T00:28:30ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.896691896691Gene Co-expression Network and Regression Analysis Identify the Transcriptomic, Physiological, and Biochemical Indicators of the Response of Alpine Woody Plant Rhododendron rex to Drought StressXiong-Li Zhou0Xiong-Li Zhou1Xiong-Li Zhou2Jin-Yan Ma3Zhen-Dian Liu4Ni-fei Dai5Hui-Qin Yang6Liu Yang7Liu Yang8Yue-Hua Wang9Yue-Hua Wang10Shi-Kang Shen11Shi-Kang Shen12Shi-Kang Shen13School of Ecology and Environmental Science, Yunnan University, Kunming, ChinaYunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology, Yunnan University, Kunming, ChinaYunnan Key Laboratory for Plateau Mountain Ecology and Restoration of Degraded Environments, Yunnan University, Kunming, ChinaSchool of Ecology and Environmental Science, Yunnan University, Kunming, ChinaSchool of Ecology and Environmental Science, Yunnan University, Kunming, ChinaSchool of Ecology and Environmental Science, Yunnan University, Kunming, ChinaSchool of Ecology and Environmental Science, Yunnan University, Kunming, ChinaSchool of Ecology and Environmental Science, Yunnan University, Kunming, ChinaYunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology, Yunnan University, Kunming, ChinaSchool of Ecology and Environmental Science, Yunnan University, Kunming, ChinaYunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology, Yunnan University, Kunming, ChinaSchool of Ecology and Environmental Science, Yunnan University, Kunming, ChinaYunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology, Yunnan University, Kunming, ChinaYunnan Key Laboratory for Plateau Mountain Ecology and Restoration of Degraded Environments, Yunnan University, Kunming, ChinaIncreasing severity of drought stress due to global change and extreme weather has been affecting the biodiversity, function, and stability of forest ecosystems. However, despite being an important component in the alpine and subalpine vegetation in forest ecosystems, Rhododendron species have been paid rare attention in the study of molecular mechanism of tolerance or response to drought. Herein, we investigated the correlation of transcriptomic changes with the physiological and biochemical indicators of Rhododendron rex under drought stress by using the co-expression network approach and regression analysis. Compared with the control treatment, the number of significantly differentially expressed unigenes (DEGs) increased with the degree of drought stress. The DEGs were mainly enriched in the cell wall metabolic process, signaling pathways, sugar metabolism, and nitrogen metabolism. Coupled analysis of the transcriptome, physiological, and biochemical parameters indicated that the metabolic pathways were highly correlated with the physiological and biochemical indicators under drought stress, especially the chlorophyll fluorescence parameters, such as the actual photosynthetic efficiency of photosystem II, electron transport rate, photochemical quenching coefficient, and the maximum quantum efficiency of photosystem II photochemistry. The majority of the response genes related to the metabolic pathways, including photosynthesis, sugar metabolism, and phytohormone signal pathway, were highly expressed under drought stress. In addition, genes associated with cell wall, pectin, and galacturonan metabolism also played crucial roles in the response of R. rex to drought stress. The results provided novel insight into the molecular response of the alpine woody species under drought stress and may improve the understanding of the response of forest ecosystems to the global climate change.https://www.frontiersin.org/articles/10.3389/fpls.2022.896691/fulldrought responsepectin biosynthesiswoody plantRhododendronclimate change |
| spellingShingle | Xiong-Li Zhou Xiong-Li Zhou Xiong-Li Zhou Jin-Yan Ma Zhen-Dian Liu Ni-fei Dai Hui-Qin Yang Liu Yang Liu Yang Yue-Hua Wang Yue-Hua Wang Shi-Kang Shen Shi-Kang Shen Shi-Kang Shen Gene Co-expression Network and Regression Analysis Identify the Transcriptomic, Physiological, and Biochemical Indicators of the Response of Alpine Woody Plant Rhododendron rex to Drought Stress Frontiers in Plant Science drought response pectin biosynthesis woody plant Rhododendron climate change |
| title | Gene Co-expression Network and Regression Analysis Identify the Transcriptomic, Physiological, and Biochemical Indicators of the Response of Alpine Woody Plant Rhododendron rex to Drought Stress |
| title_full | Gene Co-expression Network and Regression Analysis Identify the Transcriptomic, Physiological, and Biochemical Indicators of the Response of Alpine Woody Plant Rhododendron rex to Drought Stress |
| title_fullStr | Gene Co-expression Network and Regression Analysis Identify the Transcriptomic, Physiological, and Biochemical Indicators of the Response of Alpine Woody Plant Rhododendron rex to Drought Stress |
| title_full_unstemmed | Gene Co-expression Network and Regression Analysis Identify the Transcriptomic, Physiological, and Biochemical Indicators of the Response of Alpine Woody Plant Rhododendron rex to Drought Stress |
| title_short | Gene Co-expression Network and Regression Analysis Identify the Transcriptomic, Physiological, and Biochemical Indicators of the Response of Alpine Woody Plant Rhododendron rex to Drought Stress |
| title_sort | gene co expression network and regression analysis identify the transcriptomic physiological and biochemical indicators of the response of alpine woody plant rhododendron rex to drought stress |
| topic | drought response pectin biosynthesis woody plant Rhododendron climate change |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.896691/full |
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