A Comprehensive Virulence and Resistance Characteristics of <i>Listeria monocytogenes</i> Isolated from Fish and the Fish Industry Environment
<i>Listeria monocytogenes</i> is an important pathogen, often associated with fish, that can adapt and survive in products and food processing plants, where it can persist for many years. It is a species characterized by diverse genotypic and phenotypic characteristics. Therefore, in thi...
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/4/3581 |
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| author | Arkadiusz Józef Zakrzewski Monika Kurpas Anna Zadernowska Wioleta Chajęcka-Wierzchowska Maria João Fraqueza |
| author_facet | Arkadiusz Józef Zakrzewski Monika Kurpas Anna Zadernowska Wioleta Chajęcka-Wierzchowska Maria João Fraqueza |
| author_sort | Arkadiusz Józef Zakrzewski |
| collection | DOAJ |
| description | <i>Listeria monocytogenes</i> is an important pathogen, often associated with fish, that can adapt and survive in products and food processing plants, where it can persist for many years. It is a species characterized by diverse genotypic and phenotypic characteristics. Therefore, in this study, a total of 17 <i>L. monocytogenes</i> strains from fish and fish-processing environments in Poland were characterized for their relatedness, virulence profiles, and resistance genes. The Core Genome Multilocus Sequence Typing (cgMLST) analysis revealed that the most frequent serogroups were IIa and IIb; sequence types (ST) were ST6 and ST121; and clonal complexes (CC) were CC6 and CC121. Core genome multilocus sequence typing (cgMLST) analysis was applied to compare the present isolates with the publicly available genomes of <i>L. monocytogenes</i> strains recovered in Europe from humans with listeriosis. Despite differential genotypic subtypes, most strains had similar antimicrobial resistance profiles; however, some of genes were located on mobile genetic elements that could be transferred to commensal or pathogenic bacteria. The results of this study showed that molecular clones of tested strains were characteristic for <i>L. monocytogenes</i> isolated from similar sources. Nevertheless, it is worth emphasizing that they could present a major public health risk due to their close relation with strains isolated from human listeriosis. |
| first_indexed | 2024-03-11T08:42:55Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:42:55Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6396f80c630d4c2cb7f9ccc7029780712023-11-16T21:02:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244358110.3390/ijms24043581A Comprehensive Virulence and Resistance Characteristics of <i>Listeria monocytogenes</i> Isolated from Fish and the Fish Industry EnvironmentArkadiusz Józef Zakrzewski0Monika Kurpas1Anna Zadernowska2Wioleta Chajęcka-Wierzchowska3Maria João Fraqueza4Department of Industrial and Food Microbiology, University of Warmia and Mazrui, 10-726 Olsztyn, PolandDepartment of Immunobiology and Environmental Microbiology, Medical University of Gdansk, 80-210 Gdansk, PolandDepartment of Industrial and Food Microbiology, University of Warmia and Mazrui, 10-726 Olsztyn, PolandDepartment of Industrial and Food Microbiology, University of Warmia and Mazrui, 10-726 Olsztyn, PolandCIISA—Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, 1749-016 Lisboa, Portugal<i>Listeria monocytogenes</i> is an important pathogen, often associated with fish, that can adapt and survive in products and food processing plants, where it can persist for many years. It is a species characterized by diverse genotypic and phenotypic characteristics. Therefore, in this study, a total of 17 <i>L. monocytogenes</i> strains from fish and fish-processing environments in Poland were characterized for their relatedness, virulence profiles, and resistance genes. The Core Genome Multilocus Sequence Typing (cgMLST) analysis revealed that the most frequent serogroups were IIa and IIb; sequence types (ST) were ST6 and ST121; and clonal complexes (CC) were CC6 and CC121. Core genome multilocus sequence typing (cgMLST) analysis was applied to compare the present isolates with the publicly available genomes of <i>L. monocytogenes</i> strains recovered in Europe from humans with listeriosis. Despite differential genotypic subtypes, most strains had similar antimicrobial resistance profiles; however, some of genes were located on mobile genetic elements that could be transferred to commensal or pathogenic bacteria. The results of this study showed that molecular clones of tested strains were characteristic for <i>L. monocytogenes</i> isolated from similar sources. Nevertheless, it is worth emphasizing that they could present a major public health risk due to their close relation with strains isolated from human listeriosis.https://www.mdpi.com/1422-0067/24/4/3581<i>Listeria monocytogenes</i>virulenceWGSfish |
| spellingShingle | Arkadiusz Józef Zakrzewski Monika Kurpas Anna Zadernowska Wioleta Chajęcka-Wierzchowska Maria João Fraqueza A Comprehensive Virulence and Resistance Characteristics of <i>Listeria monocytogenes</i> Isolated from Fish and the Fish Industry Environment International Journal of Molecular Sciences <i>Listeria monocytogenes</i> virulence WGS fish |
| title | A Comprehensive Virulence and Resistance Characteristics of <i>Listeria monocytogenes</i> Isolated from Fish and the Fish Industry Environment |
| title_full | A Comprehensive Virulence and Resistance Characteristics of <i>Listeria monocytogenes</i> Isolated from Fish and the Fish Industry Environment |
| title_fullStr | A Comprehensive Virulence and Resistance Characteristics of <i>Listeria monocytogenes</i> Isolated from Fish and the Fish Industry Environment |
| title_full_unstemmed | A Comprehensive Virulence and Resistance Characteristics of <i>Listeria monocytogenes</i> Isolated from Fish and the Fish Industry Environment |
| title_short | A Comprehensive Virulence and Resistance Characteristics of <i>Listeria monocytogenes</i> Isolated from Fish and the Fish Industry Environment |
| title_sort | comprehensive virulence and resistance characteristics of i listeria monocytogenes i isolated from fish and the fish industry environment |
| topic | <i>Listeria monocytogenes</i> virulence WGS fish |
| url | https://www.mdpi.com/1422-0067/24/4/3581 |
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