Microencapsulated Chitosan-Based Nanocapsules: A New Platform for Pulmonary Gene Delivery
In this work, we propose chitosan (CS)-based nanocapsules (NCs) for pulmonary gene delivery. Hyaluronic acid (HA) was incorporated in the NCs composition (HA/CS NCs) aiming to promote gene transfection in the lung epithelium. NCs were loaded with a model plasmid (pCMV-βGal) to easily evaluate their...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/9/1377 |
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| author | Estefanía Fernández-Paz Lucía Feijoo-Siota Maria Manuela Gaspar Noemi Csaba Carmen Remuñán-López |
| author_facet | Estefanía Fernández-Paz Lucía Feijoo-Siota Maria Manuela Gaspar Noemi Csaba Carmen Remuñán-López |
| author_sort | Estefanía Fernández-Paz |
| collection | DOAJ |
| description | In this work, we propose chitosan (CS)-based nanocapsules (NCs) for pulmonary gene delivery. Hyaluronic acid (HA) was incorporated in the NCs composition (HA/CS NCs) aiming to promote gene transfection in the lung epithelium. NCs were loaded with a model plasmid (pCMV-βGal) to easily evaluate their transfection capacity. The plasmid encapsulation efficiencies were of approx. 90%. To facilitate their administration to the lungs, the plasmid-loaded NCs were microencapsulated in mannitol (Ma) microspheres (MS) using a simple spray-drying technique, obtaining dry powders of adequate properties. In vivo, the MS reached the deep lung, where the plasmid-loaded CS-based NCs were released and transfected the alveolar cells more homogeneously than the control formulation of plasmid directly microencapsulated in Ma MS. The HA-containing formulation achieved the highest transfection efficiency, in a more extended area and more homogeneously distributed than the rest of tested formulations. The new micro-nanostructured platform proposed in this work represents an efficient strategy for the delivery of genetic material to the lung, with great potential for the treatment of genetic lung diseases. |
| first_indexed | 2024-03-10T07:18:18Z |
| format | Article |
| id | doaj.art-63997b4b1b9646ff9809c2e257dcbe8d |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T07:18:18Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-63997b4b1b9646ff9809c2e257dcbe8d2023-11-22T14:46:44ZengMDPI AGPharmaceutics1999-49232021-08-01139137710.3390/pharmaceutics13091377Microencapsulated Chitosan-Based Nanocapsules: A New Platform for Pulmonary Gene DeliveryEstefanía Fernández-Paz0Lucía Feijoo-Siota1Maria Manuela Gaspar2Noemi Csaba3Carmen Remuñán-López4Nanobiofar Group, Department of Pharmacology, Pharmacy & Pharmaceutical Technology, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, SpainDepartment of Microbiology & Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, SpainResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, University of Lisboa, Av. Professor GamaPinto, 1649-003 Lisbon, PortugalNanobiofar Group, Center of Research in Molecular Medicine and Chronic Diseases (CiMUS), University of Santiago de Compostela, Campus Vida, 15706 Santiago de Compostela, SpainNanobiofar Group, Department of Pharmacology, Pharmacy & Pharmaceutical Technology, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, SpainIn this work, we propose chitosan (CS)-based nanocapsules (NCs) for pulmonary gene delivery. Hyaluronic acid (HA) was incorporated in the NCs composition (HA/CS NCs) aiming to promote gene transfection in the lung epithelium. NCs were loaded with a model plasmid (pCMV-βGal) to easily evaluate their transfection capacity. The plasmid encapsulation efficiencies were of approx. 90%. To facilitate their administration to the lungs, the plasmid-loaded NCs were microencapsulated in mannitol (Ma) microspheres (MS) using a simple spray-drying technique, obtaining dry powders of adequate properties. In vivo, the MS reached the deep lung, where the plasmid-loaded CS-based NCs were released and transfected the alveolar cells more homogeneously than the control formulation of plasmid directly microencapsulated in Ma MS. The HA-containing formulation achieved the highest transfection efficiency, in a more extended area and more homogeneously distributed than the rest of tested formulations. The new micro-nanostructured platform proposed in this work represents an efficient strategy for the delivery of genetic material to the lung, with great potential for the treatment of genetic lung diseases.https://www.mdpi.com/1999-4923/13/9/1377chitosan nanocapsulesin vivo studymicrospherespCMV-βGalpulmonary gene deliveryspray-drying |
| spellingShingle | Estefanía Fernández-Paz Lucía Feijoo-Siota Maria Manuela Gaspar Noemi Csaba Carmen Remuñán-López Microencapsulated Chitosan-Based Nanocapsules: A New Platform for Pulmonary Gene Delivery Pharmaceutics chitosan nanocapsules in vivo study microspheres pCMV-βGal pulmonary gene delivery spray-drying |
| title | Microencapsulated Chitosan-Based Nanocapsules: A New Platform for Pulmonary Gene Delivery |
| title_full | Microencapsulated Chitosan-Based Nanocapsules: A New Platform for Pulmonary Gene Delivery |
| title_fullStr | Microencapsulated Chitosan-Based Nanocapsules: A New Platform for Pulmonary Gene Delivery |
| title_full_unstemmed | Microencapsulated Chitosan-Based Nanocapsules: A New Platform for Pulmonary Gene Delivery |
| title_short | Microencapsulated Chitosan-Based Nanocapsules: A New Platform for Pulmonary Gene Delivery |
| title_sort | microencapsulated chitosan based nanocapsules a new platform for pulmonary gene delivery |
| topic | chitosan nanocapsules in vivo study microspheres pCMV-βGal pulmonary gene delivery spray-drying |
| url | https://www.mdpi.com/1999-4923/13/9/1377 |
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