Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples
Magnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles (MNPs) have great potential for nucleic acid separation, detection, and delivery. MNPs are considered a valuable tool in biomedicine due to their cost-effectiveness, stability, easy surface functionalization, and the possibil...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Magnetochemistry |
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| Online Access: | https://www.mdpi.com/2312-7481/8/8/85 |
| _version_ | 1797431959178182656 |
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| author | Anastasia Bulgakova Alexey Chubarov Elena Dmitrienko |
| author_facet | Anastasia Bulgakova Alexey Chubarov Elena Dmitrienko |
| author_sort | Anastasia Bulgakova |
| collection | DOAJ |
| description | Magnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles (MNPs) have great potential for nucleic acid separation, detection, and delivery. MNPs are considered a valuable tool in biomedicine due to their cost-effectiveness, stability, easy surface functionalization, and the possibility of the manipulations under a magnetic field. Herein, the synthesis of magnetic nylon 6 nanocomposites (MNPs@Ny6) was investigated. Transmission electron microscopy (TEM) was used for morphology and size analysis. A new method of UV-induced immobilization of oligonucleotides on MNPs@Ny6 for nucleic acid magnetic separation was proposed. MNPs@Ny6 shows a high oligonucleotide binding capacity of 2.2 nmol/mg with 73.3% loading efficiency. The proposed system has been applied to analyze model mixtures of target RNA on the total yeast RNA background. The RNA target isolation efficiency was 60% with high specificity. The bind RNA release was 88.8% in a quantity of 0.16 nmol/mg. The total RNA capture efficiency was 53%. Considering this, the MNPs@Ny6 is an attractive candidate for nucleic acids-specific magnetic isolation. |
| first_indexed | 2024-03-09T09:53:51Z |
| format | Article |
| id | doaj.art-639f1eb5ed654ccd9c91623d37c54da3 |
| institution | Directory Open Access Journal |
| issn | 2312-7481 |
| language | English |
| last_indexed | 2024-03-09T09:53:51Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Magnetochemistry |
| spelling | doaj.art-639f1eb5ed654ccd9c91623d37c54da32023-12-01T23:55:49ZengMDPI AGMagnetochemistry2312-74812022-08-01888510.3390/magnetochemistry8080085Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological SamplesAnastasia Bulgakova0Alexey Chubarov1Elena Dmitrienko2Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaMagnetic Fe<sub>3</sub>O<sub>4</sub> nanoparticles (MNPs) have great potential for nucleic acid separation, detection, and delivery. MNPs are considered a valuable tool in biomedicine due to their cost-effectiveness, stability, easy surface functionalization, and the possibility of the manipulations under a magnetic field. Herein, the synthesis of magnetic nylon 6 nanocomposites (MNPs@Ny6) was investigated. Transmission electron microscopy (TEM) was used for morphology and size analysis. A new method of UV-induced immobilization of oligonucleotides on MNPs@Ny6 for nucleic acid magnetic separation was proposed. MNPs@Ny6 shows a high oligonucleotide binding capacity of 2.2 nmol/mg with 73.3% loading efficiency. The proposed system has been applied to analyze model mixtures of target RNA on the total yeast RNA background. The RNA target isolation efficiency was 60% with high specificity. The bind RNA release was 88.8% in a quantity of 0.16 nmol/mg. The total RNA capture efficiency was 53%. Considering this, the MNPs@Ny6 is an attractive candidate for nucleic acids-specific magnetic isolation.https://www.mdpi.com/2312-7481/8/8/85iron oxide nanoparticlesfunctionalizationnyloncoatingpolymer nanocompositesnanomedicine |
| spellingShingle | Anastasia Bulgakova Alexey Chubarov Elena Dmitrienko Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples Magnetochemistry iron oxide nanoparticles functionalization nylon coating polymer nanocomposites nanomedicine |
| title | Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples |
| title_full | Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples |
| title_fullStr | Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples |
| title_full_unstemmed | Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples |
| title_short | Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples |
| title_sort | magnetic nylon 6 nanocomposites for the microextraction of nucleic acids from biological samples |
| topic | iron oxide nanoparticles functionalization nylon coating polymer nanocomposites nanomedicine |
| url | https://www.mdpi.com/2312-7481/8/8/85 |
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