Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC)
Cannabis has been used historically for both medicinal and recreational purposes, with the most notable cannabinoids being cannabidiol (CBD) and tetrahydrocannabinol (THC). Although their therapeutic effects have been well studied and their recreational use is highly debated, the underlying mechanis...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Proteomes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-7382/11/4/36 |
| _version_ | 1827573702586269696 |
|---|---|
| author | Morteza Abyadeh Vivek Gupta Xinyue Liu Valentina Rossio Mehdi Mirzaei Jennifer Cornish Joao A. Paulo Paul A. Haynes |
| author_facet | Morteza Abyadeh Vivek Gupta Xinyue Liu Valentina Rossio Mehdi Mirzaei Jennifer Cornish Joao A. Paulo Paul A. Haynes |
| author_sort | Morteza Abyadeh |
| collection | DOAJ |
| description | Cannabis has been used historically for both medicinal and recreational purposes, with the most notable cannabinoids being cannabidiol (CBD) and tetrahydrocannabinol (THC). Although their therapeutic effects have been well studied and their recreational use is highly debated, the underlying mechanisms of their biological effects remain poorly defined. In this study, we use isobaric tag-based sample multiplexed proteome profiling to investigate protein abundance differences in the human neuroblastoma SH-SY5Y cell line treated with CBD and THC. We identified significantly regulated proteins by each treatment and performed a pathway classification and associated protein–protein interaction analysis. Our findings suggest that these treatments may lead to mitochondrial dysfunction and induce endoplasmic reticulum stress. These data can potentially be interrogated further to investigate the potential role of CBD and THC in various biological and disease contexts, providing a foundation for future studies. |
| first_indexed | 2024-03-08T20:25:00Z |
| format | Article |
| id | doaj.art-639fc237a66d4cd2a5da1d06327e436a |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-03-08T20:25:00Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-639fc237a66d4cd2a5da1d06327e436a2023-12-22T14:37:57ZengMDPI AGProteomes2227-73822023-11-011143610.3390/proteomes11040036Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC)Morteza Abyadeh0Vivek Gupta1Xinyue Liu2Valentina Rossio3Mehdi Mirzaei4Jennifer Cornish5Joao A. Paulo6Paul A. Haynes7ProGene Technologies Pty Ltd., Macquarie Park, NSW 2113, AustraliaMacquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW 2109, AustraliaDepartment of Cell Biology, Harvard Medical School, Boston, MA 02115, USADepartment of Cell Biology, Harvard Medical School, Boston, MA 02115, USAMacquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW 2109, AustraliaSchool of Psychological Sciences, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW 2109, AustraliaDepartment of Cell Biology, Harvard Medical School, Boston, MA 02115, USASchool of Natural Sciences, Macquarie University, North Ryde, NSW 2109, AustraliaCannabis has been used historically for both medicinal and recreational purposes, with the most notable cannabinoids being cannabidiol (CBD) and tetrahydrocannabinol (THC). Although their therapeutic effects have been well studied and their recreational use is highly debated, the underlying mechanisms of their biological effects remain poorly defined. In this study, we use isobaric tag-based sample multiplexed proteome profiling to investigate protein abundance differences in the human neuroblastoma SH-SY5Y cell line treated with CBD and THC. We identified significantly regulated proteins by each treatment and performed a pathway classification and associated protein–protein interaction analysis. Our findings suggest that these treatments may lead to mitochondrial dysfunction and induce endoplasmic reticulum stress. These data can potentially be interrogated further to investigate the potential role of CBD and THC in various biological and disease contexts, providing a foundation for future studies.https://www.mdpi.com/2227-7382/11/4/36tandem mass tagsTMTproreal-time database searchingunfolded protein responsehigh-field asymmetric ion mobility spectrometryquantitative proteomics |
| spellingShingle | Morteza Abyadeh Vivek Gupta Xinyue Liu Valentina Rossio Mehdi Mirzaei Jennifer Cornish Joao A. Paulo Paul A. Haynes Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC) Proteomes tandem mass tags TMTpro real-time database searching unfolded protein response high-field asymmetric ion mobility spectrometry quantitative proteomics |
| title | Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC) |
| title_full | Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC) |
| title_fullStr | Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC) |
| title_full_unstemmed | Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC) |
| title_short | Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC) |
| title_sort | proteome wide profiling using sample multiplexing of a human cell line treated with cannabidiol cbd and tetrahydrocannabinol thc |
| topic | tandem mass tags TMTpro real-time database searching unfolded protein response high-field asymmetric ion mobility spectrometry quantitative proteomics |
| url | https://www.mdpi.com/2227-7382/11/4/36 |
| work_keys_str_mv | AT mortezaabyadeh proteomewideprofilingusingsamplemultiplexingofahumancelllinetreatedwithcannabidiolcbdandtetrahydrocannabinolthc AT vivekgupta proteomewideprofilingusingsamplemultiplexingofahumancelllinetreatedwithcannabidiolcbdandtetrahydrocannabinolthc AT xinyueliu proteomewideprofilingusingsamplemultiplexingofahumancelllinetreatedwithcannabidiolcbdandtetrahydrocannabinolthc AT valentinarossio proteomewideprofilingusingsamplemultiplexingofahumancelllinetreatedwithcannabidiolcbdandtetrahydrocannabinolthc AT mehdimirzaei proteomewideprofilingusingsamplemultiplexingofahumancelllinetreatedwithcannabidiolcbdandtetrahydrocannabinolthc AT jennifercornish proteomewideprofilingusingsamplemultiplexingofahumancelllinetreatedwithcannabidiolcbdandtetrahydrocannabinolthc AT joaoapaulo proteomewideprofilingusingsamplemultiplexingofahumancelllinetreatedwithcannabidiolcbdandtetrahydrocannabinolthc AT paulahaynes proteomewideprofilingusingsamplemultiplexingofahumancelllinetreatedwithcannabidiolcbdandtetrahydrocannabinolthc |