Generation of induced pluripotent stem cell line, NIMHi009-A, from PBMCs of an adult healthy male
Human induced pluripotent stem cells provide an exceptional platform for studying pathogenesis in vitro. We, therefore, have generated and characterized human induced pluripotent stem cell (iPSC) line NIMHi009-A derived from peripheral blood mononuclear cells (PBMCs) of healthy adult male control fo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-04-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124000473 |
| _version_ | 1797303384994217984 |
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| author | Gautham Arunachal Madhura Milind Nimonkar Kenchaiah Raghavendra Ghati K Chetan Bhupesh Mehta Yogananda S. Markandeya |
| author_facet | Gautham Arunachal Madhura Milind Nimonkar Kenchaiah Raghavendra Ghati K Chetan Bhupesh Mehta Yogananda S. Markandeya |
| author_sort | Gautham Arunachal |
| collection | DOAJ |
| description | Human induced pluripotent stem cells provide an exceptional platform for studying pathogenesis in vitro. We, therefore, have generated and characterized human induced pluripotent stem cell (iPSC) line NIMHi009-A derived from peripheral blood mononuclear cells (PBMCs) of healthy adult male control for an epileptic patient carrying voltage gated sodium channel mutation, using Sendai virus-based reprogramming. The generated iPSCs express pluripotency genes and can spontaneously differentiate into three germ layers. These cells display a normal karyotype and are free of mycoplasma. The iPSC line NIMHi009-A can be used as healthy control for modelling various diseases and screening for drugs. |
| first_indexed | 2024-03-07T23:51:59Z |
| format | Article |
| id | doaj.art-63a6131ccdd945d2b97b983b94400401 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-03-07T23:51:59Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-63a6131ccdd945d2b97b983b944004012024-02-19T04:13:20ZengElsevierStem Cell Research1873-50612024-04-0176103349Generation of induced pluripotent stem cell line, NIMHi009-A, from PBMCs of an adult healthy maleGautham Arunachal0Madhura Milind Nimonkar1Kenchaiah Raghavendra2Ghati K Chetan3Bhupesh Mehta4Yogananda S. Markandeya5Department of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru, Karnataka, IndiaDepartment of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, IndiaDepartment of Neurology, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, IndiaDepartment of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru, Karnataka, IndiaDepartment of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, IndiaDepartment of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India; Corresponding author.Human induced pluripotent stem cells provide an exceptional platform for studying pathogenesis in vitro. We, therefore, have generated and characterized human induced pluripotent stem cell (iPSC) line NIMHi009-A derived from peripheral blood mononuclear cells (PBMCs) of healthy adult male control for an epileptic patient carrying voltage gated sodium channel mutation, using Sendai virus-based reprogramming. The generated iPSCs express pluripotency genes and can spontaneously differentiate into three germ layers. These cells display a normal karyotype and are free of mycoplasma. The iPSC line NIMHi009-A can be used as healthy control for modelling various diseases and screening for drugs.http://www.sciencedirect.com/science/article/pii/S1873506124000473 |
| spellingShingle | Gautham Arunachal Madhura Milind Nimonkar Kenchaiah Raghavendra Ghati K Chetan Bhupesh Mehta Yogananda S. Markandeya Generation of induced pluripotent stem cell line, NIMHi009-A, from PBMCs of an adult healthy male Stem Cell Research |
| title | Generation of induced pluripotent stem cell line, NIMHi009-A, from PBMCs of an adult healthy male |
| title_full | Generation of induced pluripotent stem cell line, NIMHi009-A, from PBMCs of an adult healthy male |
| title_fullStr | Generation of induced pluripotent stem cell line, NIMHi009-A, from PBMCs of an adult healthy male |
| title_full_unstemmed | Generation of induced pluripotent stem cell line, NIMHi009-A, from PBMCs of an adult healthy male |
| title_short | Generation of induced pluripotent stem cell line, NIMHi009-A, from PBMCs of an adult healthy male |
| title_sort | generation of induced pluripotent stem cell line nimhi009 a from pbmcs of an adult healthy male |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124000473 |
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