Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters
Abstract Routine monitoring of kidney transplant function is required for the standard care in post-transplantation management, including frequent measurements of serum creatinine with or without kidney biopsy. However, the invasiveness of these methods with potential for clinically significant comp...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-01-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-82113-7 |
| _version_ | 1818420548545282048 |
|---|---|
| author | Zhongli Huang Shijian Feng Qiunong Guan Tao Lin Jianhua Zhao Christopher Y. C. Nguan Haishan Zeng David Harriman Hong Li Caigan Du |
| author_facet | Zhongli Huang Shijian Feng Qiunong Guan Tao Lin Jianhua Zhao Christopher Y. C. Nguan Haishan Zeng David Harriman Hong Li Caigan Du |
| author_sort | Zhongli Huang |
| collection | DOAJ |
| description | Abstract Routine monitoring of kidney transplant function is required for the standard care in post-transplantation management, including frequent measurements of serum creatinine with or without kidney biopsy. However, the invasiveness of these methods with potential for clinically significant complications makes them less than ideal. The objective of this study was to develop a non-invasive tool to monitor the kidney transplant function by using Surface-Enhanced Raman Spectroscopy (SERS). Urine and blood samples were collected from kidney transplant recipients after surgery. Silver nanoparticle-based SERS spectra of the urine were measured and evaluated using partial least squires (PLS) analysis. The SERS spectra were compared with conventional chemical markers of kidney transplant function to assess its predictive ability. A total of 110 kidney transplant recipients were included in this study. PLS results showed significant correlation with urine protein (R 2 = 0.4660, p < 0.01), creatinine (R 2 = 0.8106, p < 0.01), and urea (R 2 = 0.7808, p < 0.01). Furthermore, the prediction of the blood markers of kidney transplant function using the urine SERS spectra was indicated by R 2 = 0.7628 (p < 0.01) for serum creatinine and R 2 = 0.6539 (p < 0.01) for blood urea nitrogen. This preliminary study suggested that the urine SERS spectral analysis could be used as a convenient method for rapid assessment of kidney transplant function. |
| first_indexed | 2024-12-14T12:56:13Z |
| format | Article |
| id | doaj.art-63a63548fa2b44d1badfac02f870eb37 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T12:56:13Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-63a63548fa2b44d1badfac02f870eb372022-12-21T23:00:33ZengNature PortfolioScientific Reports2045-23222021-01-011111710.1038/s41598-021-82113-7Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parametersZhongli Huang0Shijian Feng1Qiunong Guan2Tao Lin3Jianhua Zhao4Christopher Y. C. Nguan5Haishan Zeng6David Harriman7Hong Li8Caigan Du9Department of Urology, Institute of Urology, Organ Transplantation Center, West China Hospital, Sichuan UniversityDepartment of Urology, Institute of Urology, Organ Transplantation Center, West China Hospital, Sichuan UniversityDepartment of Urologic Sciences, University of British ColumbiaDepartment of Urology, Institute of Urology, Organ Transplantation Center, West China Hospital, Sichuan UniversityImaging Unit, Integrative Oncology Department, BC Cancer Research CenterDepartment of Urologic Sciences, University of British ColumbiaImaging Unit, Integrative Oncology Department, BC Cancer Research CenterDepartment of Urologic Sciences, University of British ColumbiaDepartment of Urology, Institute of Urology, Organ Transplantation Center, West China Hospital, Sichuan UniversityDepartment of Urologic Sciences, University of British ColumbiaAbstract Routine monitoring of kidney transplant function is required for the standard care in post-transplantation management, including frequent measurements of serum creatinine with or without kidney biopsy. However, the invasiveness of these methods with potential for clinically significant complications makes them less than ideal. The objective of this study was to develop a non-invasive tool to monitor the kidney transplant function by using Surface-Enhanced Raman Spectroscopy (SERS). Urine and blood samples were collected from kidney transplant recipients after surgery. Silver nanoparticle-based SERS spectra of the urine were measured and evaluated using partial least squires (PLS) analysis. The SERS spectra were compared with conventional chemical markers of kidney transplant function to assess its predictive ability. A total of 110 kidney transplant recipients were included in this study. PLS results showed significant correlation with urine protein (R 2 = 0.4660, p < 0.01), creatinine (R 2 = 0.8106, p < 0.01), and urea (R 2 = 0.7808, p < 0.01). Furthermore, the prediction of the blood markers of kidney transplant function using the urine SERS spectra was indicated by R 2 = 0.7628 (p < 0.01) for serum creatinine and R 2 = 0.6539 (p < 0.01) for blood urea nitrogen. This preliminary study suggested that the urine SERS spectral analysis could be used as a convenient method for rapid assessment of kidney transplant function.https://doi.org/10.1038/s41598-021-82113-7 |
| spellingShingle | Zhongli Huang Shijian Feng Qiunong Guan Tao Lin Jianhua Zhao Christopher Y. C. Nguan Haishan Zeng David Harriman Hong Li Caigan Du Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters Scientific Reports |
| title | Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters |
| title_full | Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters |
| title_fullStr | Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters |
| title_full_unstemmed | Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters |
| title_short | Correlation of surface-enhanced Raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters |
| title_sort | correlation of surface enhanced raman spectroscopic fingerprints of kidney transplant recipient urine with kidney function parameters |
| url | https://doi.org/10.1038/s41598-021-82113-7 |
| work_keys_str_mv | AT zhonglihuang correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT shijianfeng correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT qiunongguan correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT taolin correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT jianhuazhao correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT christopherycnguan correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT haishanzeng correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT davidharriman correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT hongli correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters AT caigandu correlationofsurfaceenhancedramanspectroscopicfingerprintsofkidneytransplantrecipienturinewithkidneyfunctionparameters |