Male versus female inflammatory response after brain death model followed by ex vivo lung perfusion
Abstract Background Ex vivo lung perfusion (EVLP) is a useful tool for assessing lung grafts quality before transplantation. Studies indicate that donor sex is as an important factor for transplant outcome, as females present higher inflammatory response to brain death (BD) than males. Here, we inve...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-01-01
|
| Series: | Biology of Sex Differences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13293-024-00581-8 |
| _version_ | 1797276126864736256 |
|---|---|
| author | Fernanda Yamamoto Ricardo-da-Silva Roberto Armstrong-Jr Mayara Munhoz de Assis Ramos Marina Vidal-dos-Santos Cristiano Jesus Correia Petra J. Ottens Luiz Felipe Pinho Moreira Henri G. D. Leuvenink Ana Cristina Breithaupt-Faloppa |
| author_facet | Fernanda Yamamoto Ricardo-da-Silva Roberto Armstrong-Jr Mayara Munhoz de Assis Ramos Marina Vidal-dos-Santos Cristiano Jesus Correia Petra J. Ottens Luiz Felipe Pinho Moreira Henri G. D. Leuvenink Ana Cristina Breithaupt-Faloppa |
| author_sort | Fernanda Yamamoto Ricardo-da-Silva |
| collection | DOAJ |
| description | Abstract Background Ex vivo lung perfusion (EVLP) is a useful tool for assessing lung grafts quality before transplantation. Studies indicate that donor sex is as an important factor for transplant outcome, as females present higher inflammatory response to brain death (BD) than males. Here, we investigated sex differences in the lungs of rats subjected to BD followed by EVLP. Methods Male and female Wistar rats were subjected to BD, and as controls sham animals. Arterial blood was sampled for gas analysis. Heart–lung blocks were kept in cold storage (1 h) and normothermic EVLP carried out (4 h), meanwhile ventilation parameters were recorded. Perfusate was sampled for gas analysis and IL-1β levels. Leukocyte infiltration, myeloperoxidase presence, IL-1β gene expression, and long-term release in lung culture (explant) were evaluated. Results Brain dead females presented a low lung function after BD, compared to BD-males; however, at the end of the EVLP period oxygenation capacity decreased in all BD groups. Overall, ventilation parameters were maintained in all groups. After EVLP lung infiltrate was higher in brain dead females, with higher neutrophil content, and accompanied by high IL-1β levels, with increased gene expression and concentration in the culture medium (explant) 24 h after EVLP. Female rats presented higher lung inflammation after BD than male rats. Despite maintaining lung function and ventilation mechanics parameters for 4 h, EVLP was not able to alter this profile. Conclusion In this context, further studies should focus on therapeutic measures to control inflammation in donor or during EVLP to increase lung quality. |
| first_indexed | 2024-03-07T15:23:51Z |
| format | Article |
| id | doaj.art-63a6ac079b8340ee8222e94aca304d7c |
| institution | Directory Open Access Journal |
| issn | 2042-6410 |
| language | English |
| last_indexed | 2024-03-07T15:23:51Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biology of Sex Differences |
| spelling | doaj.art-63a6ac079b8340ee8222e94aca304d7c2024-03-05T17:26:22ZengBMCBiology of Sex Differences2042-64102024-01-0115111310.1186/s13293-024-00581-8Male versus female inflammatory response after brain death model followed by ex vivo lung perfusionFernanda Yamamoto Ricardo-da-Silva0Roberto Armstrong-Jr1Mayara Munhoz de Assis Ramos2Marina Vidal-dos-Santos3Cristiano Jesus Correia4Petra J. Ottens5Luiz Felipe Pinho Moreira6Henri G. D. Leuvenink7Ana Cristina Breithaupt-Faloppa8Laboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), HC-FMUSP, Instituto Do Coração (InCor), Faculdade de Medicina da Universidade de São PauloLaboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), HC-FMUSP, Instituto Do Coração (InCor), Faculdade de Medicina da Universidade de São PauloLaboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), HC-FMUSP, Instituto Do Coração (InCor), Faculdade de Medicina da Universidade de São PauloLaboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), HC-FMUSP, Instituto Do Coração (InCor), Faculdade de Medicina da Universidade de São PauloLaboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), HC-FMUSP, Instituto Do Coração (InCor), Faculdade de Medicina da Universidade de São PauloDepartment of Surgery, University Medical Centre Groningen, University of GroningenLaboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), HC-FMUSP, Instituto Do Coração (InCor), Faculdade de Medicina da Universidade de São PauloDepartment of Surgery, University Medical Centre Groningen, University of GroningenLaboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circulação (LIM-11), HC-FMUSP, Instituto Do Coração (InCor), Faculdade de Medicina da Universidade de São PauloAbstract Background Ex vivo lung perfusion (EVLP) is a useful tool for assessing lung grafts quality before transplantation. Studies indicate that donor sex is as an important factor for transplant outcome, as females present higher inflammatory response to brain death (BD) than males. Here, we investigated sex differences in the lungs of rats subjected to BD followed by EVLP. Methods Male and female Wistar rats were subjected to BD, and as controls sham animals. Arterial blood was sampled for gas analysis. Heart–lung blocks were kept in cold storage (1 h) and normothermic EVLP carried out (4 h), meanwhile ventilation parameters were recorded. Perfusate was sampled for gas analysis and IL-1β levels. Leukocyte infiltration, myeloperoxidase presence, IL-1β gene expression, and long-term release in lung culture (explant) were evaluated. Results Brain dead females presented a low lung function after BD, compared to BD-males; however, at the end of the EVLP period oxygenation capacity decreased in all BD groups. Overall, ventilation parameters were maintained in all groups. After EVLP lung infiltrate was higher in brain dead females, with higher neutrophil content, and accompanied by high IL-1β levels, with increased gene expression and concentration in the culture medium (explant) 24 h after EVLP. Female rats presented higher lung inflammation after BD than male rats. Despite maintaining lung function and ventilation mechanics parameters for 4 h, EVLP was not able to alter this profile. Conclusion In this context, further studies should focus on therapeutic measures to control inflammation in donor or during EVLP to increase lung quality.https://doi.org/10.1186/s13293-024-00581-8Brain deathDonorEx vivo lung perfusionLungRatSex |
| spellingShingle | Fernanda Yamamoto Ricardo-da-Silva Roberto Armstrong-Jr Mayara Munhoz de Assis Ramos Marina Vidal-dos-Santos Cristiano Jesus Correia Petra J. Ottens Luiz Felipe Pinho Moreira Henri G. D. Leuvenink Ana Cristina Breithaupt-Faloppa Male versus female inflammatory response after brain death model followed by ex vivo lung perfusion Biology of Sex Differences Brain death Donor Ex vivo lung perfusion Lung Rat Sex |
| title | Male versus female inflammatory response after brain death model followed by ex vivo lung perfusion |
| title_full | Male versus female inflammatory response after brain death model followed by ex vivo lung perfusion |
| title_fullStr | Male versus female inflammatory response after brain death model followed by ex vivo lung perfusion |
| title_full_unstemmed | Male versus female inflammatory response after brain death model followed by ex vivo lung perfusion |
| title_short | Male versus female inflammatory response after brain death model followed by ex vivo lung perfusion |
| title_sort | male versus female inflammatory response after brain death model followed by ex vivo lung perfusion |
| topic | Brain death Donor Ex vivo lung perfusion Lung Rat Sex |
| url | https://doi.org/10.1186/s13293-024-00581-8 |
| work_keys_str_mv | AT fernandayamamotoricardodasilva maleversusfemaleinflammatoryresponseafterbraindeathmodelfollowedbyexvivolungperfusion AT robertoarmstrongjr maleversusfemaleinflammatoryresponseafterbraindeathmodelfollowedbyexvivolungperfusion AT mayaramunhozdeassisramos maleversusfemaleinflammatoryresponseafterbraindeathmodelfollowedbyexvivolungperfusion AT marinavidaldossantos maleversusfemaleinflammatoryresponseafterbraindeathmodelfollowedbyexvivolungperfusion AT cristianojesuscorreia maleversusfemaleinflammatoryresponseafterbraindeathmodelfollowedbyexvivolungperfusion AT petrajottens maleversusfemaleinflammatoryresponseafterbraindeathmodelfollowedbyexvivolungperfusion AT luizfelipepinhomoreira maleversusfemaleinflammatoryresponseafterbraindeathmodelfollowedbyexvivolungperfusion AT henrigdleuvenink maleversusfemaleinflammatoryresponseafterbraindeathmodelfollowedbyexvivolungperfusion AT anacristinabreithauptfaloppa maleversusfemaleinflammatoryresponseafterbraindeathmodelfollowedbyexvivolungperfusion |