Long segment detection of HTLV-1 genome based on the fluorescence quenching technique
Detecting fluorescence changes due to energy transfer between a quencher and fluorophore is a common method used for the fluorescence-based biosensors. In the present report, a new biosensor for long segment detection of the human T cell-lymphotropic virus 1 genome was constructed based on the fluor...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-12-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844018345705 |
| _version_ | 1818849064530214912 |
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| author | Mohadeseh Zarei Ghobadi Sayed-Hamidreza Mozhgani Fatemeh Hakimian Mehdi Norouzi Seyed Abdolrahim Rezaee Hedayatollah Ghourchian |
| author_facet | Mohadeseh Zarei Ghobadi Sayed-Hamidreza Mozhgani Fatemeh Hakimian Mehdi Norouzi Seyed Abdolrahim Rezaee Hedayatollah Ghourchian |
| author_sort | Mohadeseh Zarei Ghobadi |
| collection | DOAJ |
| description | Detecting fluorescence changes due to energy transfer between a quencher and fluorophore is a common method used for the fluorescence-based biosensors. In the present report, a new biosensor for long segment detection of the human T cell-lymphotropic virus 1 genome was constructed based on the fluorescence quenching of graphene oxide by gold nanoparticles. The fluorescence signal of unmodified graphene oxide was measured before and after hybridization of target and probes functionalized with gold nanoparticles. The limit of detection of the biosensor was determined to be around 10 pg/mL. The specific design for long segment of target assures the selectivity of biosensor. Our results proposed that further development may be useful to detect other viruses. |
| first_indexed | 2024-12-19T06:27:18Z |
| format | Article |
| id | doaj.art-63a8299da54f4dbe8d2b36f5c38a5297 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-19T06:27:18Z |
| publishDate | 2018-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-63a8299da54f4dbe8d2b36f5c38a52972022-12-21T20:32:30ZengElsevierHeliyon2405-84402018-12-01412e00996Long segment detection of HTLV-1 genome based on the fluorescence quenching techniqueMohadeseh Zarei Ghobadi0Sayed-Hamidreza Mozhgani1Fatemeh Hakimian2Mehdi Norouzi3Seyed Abdolrahim Rezaee4Hedayatollah Ghourchian5Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran; Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran; Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, IranInstitute of Biochemistry and Biophysics, University of Tehran, Tehran, IranDepartment of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, IranImmunology Research Center, Inflammation and Inflammatory Diseases Division, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranInstitute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran; Corresponding author.Detecting fluorescence changes due to energy transfer between a quencher and fluorophore is a common method used for the fluorescence-based biosensors. In the present report, a new biosensor for long segment detection of the human T cell-lymphotropic virus 1 genome was constructed based on the fluorescence quenching of graphene oxide by gold nanoparticles. The fluorescence signal of unmodified graphene oxide was measured before and after hybridization of target and probes functionalized with gold nanoparticles. The limit of detection of the biosensor was determined to be around 10 pg/mL. The specific design for long segment of target assures the selectivity of biosensor. Our results proposed that further development may be useful to detect other viruses.http://www.sciencedirect.com/science/article/pii/S2405844018345705VirologyBiotechnologyAnalytical chemistry |
| spellingShingle | Mohadeseh Zarei Ghobadi Sayed-Hamidreza Mozhgani Fatemeh Hakimian Mehdi Norouzi Seyed Abdolrahim Rezaee Hedayatollah Ghourchian Long segment detection of HTLV-1 genome based on the fluorescence quenching technique Heliyon Virology Biotechnology Analytical chemistry |
| title | Long segment detection of HTLV-1 genome based on the fluorescence quenching technique |
| title_full | Long segment detection of HTLV-1 genome based on the fluorescence quenching technique |
| title_fullStr | Long segment detection of HTLV-1 genome based on the fluorescence quenching technique |
| title_full_unstemmed | Long segment detection of HTLV-1 genome based on the fluorescence quenching technique |
| title_short | Long segment detection of HTLV-1 genome based on the fluorescence quenching technique |
| title_sort | long segment detection of htlv 1 genome based on the fluorescence quenching technique |
| topic | Virology Biotechnology Analytical chemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2405844018345705 |
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