Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: Protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques.
Lumbar disc degeneration (LDD) and low back pain (LBP) are two conditions that are closely related. Several studies have shown Cutibacterium acnes colonization of degenerated discs, but whether and how these finding correlates with LBP is unknown. A prospective study was planned to identify molecule...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0271773 |
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| author | Vinícius Magno da Rocha Carla Ormundo Gonçalves Ximenes Lima Eliane de Oliveira Ferreira Gabriel Corrêa de Farias Fábio César Sousa Nogueira Luis Caetano Martha Antunes Keila Mara Cassiano Rossano Kepler Alvim Fiorelli |
| author_facet | Vinícius Magno da Rocha Carla Ormundo Gonçalves Ximenes Lima Eliane de Oliveira Ferreira Gabriel Corrêa de Farias Fábio César Sousa Nogueira Luis Caetano Martha Antunes Keila Mara Cassiano Rossano Kepler Alvim Fiorelli |
| author_sort | Vinícius Magno da Rocha |
| collection | DOAJ |
| description | Lumbar disc degeneration (LDD) and low back pain (LBP) are two conditions that are closely related. Several studies have shown Cutibacterium acnes colonization of degenerated discs, but whether and how these finding correlates with LBP is unknown. A prospective study was planned to identify molecules present in lumbar intervertebral discs (LLIVD) colonized by C. acnes in patients with LDD and LBP and correlate them with their clinical, radiological, and demographic profiles. The clinical manifestations, risk factors, and demographic characteristics of participants undergoing surgical microdiscectomy will be tracked. Samples will be isolated and pathogens found in LLIVD will be characterized phenotypically and genotypically. Whole genome sequencing (WGS) of isolated species will be used to phylotype and detect genes associated with virulence, resistance, and oxidative stress. Multiomic analyses of LLIVD colonized and non-colonized will be carried out to explain not only the pathogen's role in LDD, but also its involvement in the pathophysiology of LBP. This study was approved by the Institutional Review Board (CAAE 50077521.0.0000.5258). All patients who agree to participate in the study will sign an informed consent form. Regardless of the study's findings, the results will be published in a peer-reviewed medical journal. Trials registration number NCT05090553; pre-results. |
| first_indexed | 2024-04-10T06:03:51Z |
| format | Article |
| id | doaj.art-63aa81f3606c4db2a0e5ad6f7a0a7814 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-10T06:03:51Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-63aa81f3606c4db2a0e5ad6f7a0a78142023-03-03T05:31:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01182e027177310.1371/journal.pone.0271773Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: Protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques.Vinícius Magno da RochaCarla Ormundo Gonçalves Ximenes LimaEliane de Oliveira FerreiraGabriel Corrêa de FariasFábio César Sousa NogueiraLuis Caetano Martha AntunesKeila Mara CassianoRossano Kepler Alvim FiorelliLumbar disc degeneration (LDD) and low back pain (LBP) are two conditions that are closely related. Several studies have shown Cutibacterium acnes colonization of degenerated discs, but whether and how these finding correlates with LBP is unknown. A prospective study was planned to identify molecules present in lumbar intervertebral discs (LLIVD) colonized by C. acnes in patients with LDD and LBP and correlate them with their clinical, radiological, and demographic profiles. The clinical manifestations, risk factors, and demographic characteristics of participants undergoing surgical microdiscectomy will be tracked. Samples will be isolated and pathogens found in LLIVD will be characterized phenotypically and genotypically. Whole genome sequencing (WGS) of isolated species will be used to phylotype and detect genes associated with virulence, resistance, and oxidative stress. Multiomic analyses of LLIVD colonized and non-colonized will be carried out to explain not only the pathogen's role in LDD, but also its involvement in the pathophysiology of LBP. This study was approved by the Institutional Review Board (CAAE 50077521.0.0000.5258). All patients who agree to participate in the study will sign an informed consent form. Regardless of the study's findings, the results will be published in a peer-reviewed medical journal. Trials registration number NCT05090553; pre-results.https://doi.org/10.1371/journal.pone.0271773 |
| spellingShingle | Vinícius Magno da Rocha Carla Ormundo Gonçalves Ximenes Lima Eliane de Oliveira Ferreira Gabriel Corrêa de Farias Fábio César Sousa Nogueira Luis Caetano Martha Antunes Keila Mara Cassiano Rossano Kepler Alvim Fiorelli Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: Protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques. PLoS ONE |
| title | Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: Protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques. |
| title_full | Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: Protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques. |
| title_fullStr | Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: Protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques. |
| title_full_unstemmed | Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: Protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques. |
| title_short | Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: Protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques. |
| title_sort | colonization of intervertebral discs by cutibacterium acnes in patients with low back pain protocol for an analytical study with microbiological phenotypic genotypic and multiomic techniques |
| url | https://doi.org/10.1371/journal.pone.0271773 |
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