Valencene-rich fraction from Vetiveria zizanioides exerts immunostimulatory effects in vitro and in mice
Objective: To decipher the responsible compound present in the aqueous root extract of Vetiveria zizanioides which has tremendous immunomodulatory activity. Methods: Different fractions of the water extract were collected and analyzed for immunomodulatory activity by analyzing in vitro phagocytic ac...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2021-01-01
|
| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=8;spage=335;epage=343;aulast=Sunitha |
| _version_ | 1797998977539375104 |
|---|---|
| author | V S Sunitha Sebastian Jose Midhun M A Sunil E K Radhakrishnan Jyothis Mathew |
| author_facet | V S Sunitha Sebastian Jose Midhun M A Sunil E K Radhakrishnan Jyothis Mathew |
| author_sort | V S Sunitha |
| collection | DOAJ |
| description | Objective: To decipher the responsible compound present in the aqueous root extract of Vetiveria zizanioides which has tremendous immunomodulatory activity.
Methods: Different fractions of the water extract were collected and analyzed for immunomodulatory activity by analyzing in vitro phagocytic activity and nitric oxide production. One fraction VF3 was selected and further analyzed for possible compounds by high performance liquid chromatography and gas chromatography coupled with a mass spectrometer. The in vitro immunomodulatory parameters such as phagocytic index, nitrite content, and tumor necrosis factor-α production in murine macrophages were analyzed. In vivo studies, sheep red blood cell induced haemagglutination titer, the number of antibody-producing cells, and sheep red blood cell induced delayed-type hypersensitivity were analyzed. Cytotoxic studies in L929 normal fibroblasts were also performed.
Results: One of the fractions, VF3, was selected and confirmed the presence of an active compound valencene. The in vitro immunomodulatory parameters were significantly (P<0.05) increased by valencene treatment. In vivo studies in Swiss albino mice showed that valencene could significantly (P<0.05) increase haemagglutination titer, the number of antibody-producing cells, and delayed-type hypersensitivity. Cytotoxic studies also showed that valencene did not cause any morphological changes and DNA damage in normal fibroblasts.
Conclusions: Valencene possesses immunomodulatory activities and can be commercially exploited for its immunostimulatory potentials. |
| first_indexed | 2024-04-11T10:57:23Z |
| format | Article |
| id | doaj.art-63accf1abc2a4ed1aae0f6f0758a73ea |
| institution | Directory Open Access Journal |
| issn | 2221-1691 2588-9222 |
| language | English |
| last_indexed | 2024-04-11T10:57:23Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-63accf1abc2a4ed1aae0f6f0758a73ea2022-12-22T04:28:43ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222021-01-0111833534310.4103/2221-1691.319568Valencene-rich fraction from Vetiveria zizanioides exerts immunostimulatory effects in vitro and in miceV S SunithaSebastian Jose MidhunM A SunilE K RadhakrishnanJyothis MathewObjective: To decipher the responsible compound present in the aqueous root extract of Vetiveria zizanioides which has tremendous immunomodulatory activity. Methods: Different fractions of the water extract were collected and analyzed for immunomodulatory activity by analyzing in vitro phagocytic activity and nitric oxide production. One fraction VF3 was selected and further analyzed for possible compounds by high performance liquid chromatography and gas chromatography coupled with a mass spectrometer. The in vitro immunomodulatory parameters such as phagocytic index, nitrite content, and tumor necrosis factor-α production in murine macrophages were analyzed. In vivo studies, sheep red blood cell induced haemagglutination titer, the number of antibody-producing cells, and sheep red blood cell induced delayed-type hypersensitivity were analyzed. Cytotoxic studies in L929 normal fibroblasts were also performed. Results: One of the fractions, VF3, was selected and confirmed the presence of an active compound valencene. The in vitro immunomodulatory parameters were significantly (P<0.05) increased by valencene treatment. In vivo studies in Swiss albino mice showed that valencene could significantly (P<0.05) increase haemagglutination titer, the number of antibody-producing cells, and delayed-type hypersensitivity. Cytotoxic studies also showed that valencene did not cause any morphological changes and DNA damage in normal fibroblasts. Conclusions: Valencene possesses immunomodulatory activities and can be commercially exploited for its immunostimulatory potentials.http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=8;spage=335;epage=343;aulast=Sunithagc-ms; immunomodulation; valencene; vetiveria zizanioides; thirst quencher |
| spellingShingle | V S Sunitha Sebastian Jose Midhun M A Sunil E K Radhakrishnan Jyothis Mathew Valencene-rich fraction from Vetiveria zizanioides exerts immunostimulatory effects in vitro and in mice Asian Pacific Journal of Tropical Biomedicine gc-ms; immunomodulation; valencene; vetiveria zizanioides; thirst quencher |
| title | Valencene-rich fraction from Vetiveria zizanioides exerts immunostimulatory effects in vitro and in mice |
| title_full | Valencene-rich fraction from Vetiveria zizanioides exerts immunostimulatory effects in vitro and in mice |
| title_fullStr | Valencene-rich fraction from Vetiveria zizanioides exerts immunostimulatory effects in vitro and in mice |
| title_full_unstemmed | Valencene-rich fraction from Vetiveria zizanioides exerts immunostimulatory effects in vitro and in mice |
| title_short | Valencene-rich fraction from Vetiveria zizanioides exerts immunostimulatory effects in vitro and in mice |
| title_sort | valencene rich fraction from vetiveria zizanioides exerts immunostimulatory effects in vitro and in mice |
| topic | gc-ms; immunomodulation; valencene; vetiveria zizanioides; thirst quencher |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=8;spage=335;epage=343;aulast=Sunitha |
| work_keys_str_mv | AT vssunitha valencenerichfractionfromvetiveriazizanioidesexertsimmunostimulatoryeffectsinvitroandinmice AT sebastianjosemidhun valencenerichfractionfromvetiveriazizanioidesexertsimmunostimulatoryeffectsinvitroandinmice AT masunil valencenerichfractionfromvetiveriazizanioidesexertsimmunostimulatoryeffectsinvitroandinmice AT ekradhakrishnan valencenerichfractionfromvetiveriazizanioidesexertsimmunostimulatoryeffectsinvitroandinmice AT jyothismathew valencenerichfractionfromvetiveriazizanioidesexertsimmunostimulatoryeffectsinvitroandinmice |