Isolation and Genetic Characterization of Parvoviruses From Dogs, Cats, Minks, and Raccoon Dogs in the Eastern Region of Shandong Province, China
The eastern region of Shandong province, China, is an intensive economic mink and raccoon dog breeding area. To investigate the molecular variations of parvovirus in cats, dogs, minks, and raccoon dogs from this region, feline panleukopenia virus (FPV), canine parvovirus 2 (CPV-2), mink enteritis vi...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.862352/full |
| _version_ | 1818987742026006528 |
|---|---|
| author | Jiakai Zhao Jiakai Zhao Hao Zhang Hao Zhang Lu Zhang Lu Zhang Qiang Zhang Qiang Zhang Ning Zhou Ning Zhou Taofeng Du Taofeng Du Qin Zhao Qin Zhao En-Min Zhou En-Min Zhou Yongkun Du Yani Sun Yani Sun |
| author_facet | Jiakai Zhao Jiakai Zhao Hao Zhang Hao Zhang Lu Zhang Lu Zhang Qiang Zhang Qiang Zhang Ning Zhou Ning Zhou Taofeng Du Taofeng Du Qin Zhao Qin Zhao En-Min Zhou En-Min Zhou Yongkun Du Yani Sun Yani Sun |
| author_sort | Jiakai Zhao |
| collection | DOAJ |
| description | The eastern region of Shandong province, China, is an intensive economic mink and raccoon dog breeding area. To investigate the molecular variations of parvovirus in cats, dogs, minks, and raccoon dogs from this region, feline panleukopenia virus (FPV), canine parvovirus 2 (CPV-2), mink enteritis virus (MEV), and raccoon dog parvovirus (RDPV) were separately isolated and characterized from the respective animals with gastroenteritis. PCR amplification showed that there were 15/18 (83.3%), 9/13 (69.2%), 8/11 (72.7%), and 3/7 (42.9%) samples from the diseased animals separately positive for FPV, CPV-2, MEV, and RDPV. Of these, a total of six FPV, six MEV, four CPV-2, and three RDPV strains were successfully isolated using F81 cells. Next, the near-complete genomes of 19 parvovirus isolates were amplified and analyzed. The viral particle 2 (VP2) sequence alignment showed that they shared 97.2–100% nucleotide similarity. Phylogenetic analysis showed that the five FPV isolates were in the same branch, and an FPV isolate was closely related with MEV and RDPV isolates obtained in this study. These suggested that cross-species infection occurred in the Shandong region between the FPV, MEV, and RDPV. For the four CPV-2 isolates, three were antigenic variant strains CPV-2a, and the other was antigenic variant strain CPV-2c. Additionally, the mutations that had emerged in the VP2 amino acids of CPV-2 also occurred in the VP2 from the FPV, MEV, and RDPV isolates. This study suggested that the continuous evolution of the parvovirus may be accelerated in areas with a high density of economic animal trading/breeding, and controlling parvovirus infection in these animals remains a challenge. |
| first_indexed | 2024-12-20T19:11:31Z |
| format | Article |
| id | doaj.art-63acf1e9e75944ac9f411b6e640837f3 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T19:11:31Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-63acf1e9e75944ac9f411b6e640837f32022-12-21T19:29:11ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-02-011310.3389/fmicb.2022.862352862352Isolation and Genetic Characterization of Parvoviruses From Dogs, Cats, Minks, and Raccoon Dogs in the Eastern Region of Shandong Province, ChinaJiakai Zhao0Jiakai Zhao1Hao Zhang2Hao Zhang3Lu Zhang4Lu Zhang5Qiang Zhang6Qiang Zhang7Ning Zhou8Ning Zhou9Taofeng Du10Taofeng Du11Qin Zhao12Qin Zhao13En-Min Zhou14En-Min Zhou15Yongkun Du16Yani Sun17Yani Sun18Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, ChinaScientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture, Yangling, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, ChinaScientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture, Yangling, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, ChinaScientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture, Yangling, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, ChinaScientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture, Yangling, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, ChinaScientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture, Yangling, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, ChinaScientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture, Yangling, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, ChinaScientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture, Yangling, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, ChinaScientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture, Yangling, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, ChinaScientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture, Yangling, ChinaThe eastern region of Shandong province, China, is an intensive economic mink and raccoon dog breeding area. To investigate the molecular variations of parvovirus in cats, dogs, minks, and raccoon dogs from this region, feline panleukopenia virus (FPV), canine parvovirus 2 (CPV-2), mink enteritis virus (MEV), and raccoon dog parvovirus (RDPV) were separately isolated and characterized from the respective animals with gastroenteritis. PCR amplification showed that there were 15/18 (83.3%), 9/13 (69.2%), 8/11 (72.7%), and 3/7 (42.9%) samples from the diseased animals separately positive for FPV, CPV-2, MEV, and RDPV. Of these, a total of six FPV, six MEV, four CPV-2, and three RDPV strains were successfully isolated using F81 cells. Next, the near-complete genomes of 19 parvovirus isolates were amplified and analyzed. The viral particle 2 (VP2) sequence alignment showed that they shared 97.2–100% nucleotide similarity. Phylogenetic analysis showed that the five FPV isolates were in the same branch, and an FPV isolate was closely related with MEV and RDPV isolates obtained in this study. These suggested that cross-species infection occurred in the Shandong region between the FPV, MEV, and RDPV. For the four CPV-2 isolates, three were antigenic variant strains CPV-2a, and the other was antigenic variant strain CPV-2c. Additionally, the mutations that had emerged in the VP2 amino acids of CPV-2 also occurred in the VP2 from the FPV, MEV, and RDPV isolates. This study suggested that the continuous evolution of the parvovirus may be accelerated in areas with a high density of economic animal trading/breeding, and controlling parvovirus infection in these animals remains a challenge.https://www.frontiersin.org/articles/10.3389/fmicb.2022.862352/fullcanine parvovirus 2feline panleukopenia virusgenetic characterizationmink enteritis virusraccoon dog parvovirusphylogenetic analysis |
| spellingShingle | Jiakai Zhao Jiakai Zhao Hao Zhang Hao Zhang Lu Zhang Lu Zhang Qiang Zhang Qiang Zhang Ning Zhou Ning Zhou Taofeng Du Taofeng Du Qin Zhao Qin Zhao En-Min Zhou En-Min Zhou Yongkun Du Yani Sun Yani Sun Isolation and Genetic Characterization of Parvoviruses From Dogs, Cats, Minks, and Raccoon Dogs in the Eastern Region of Shandong Province, China Frontiers in Microbiology canine parvovirus 2 feline panleukopenia virus genetic characterization mink enteritis virus raccoon dog parvovirus phylogenetic analysis |
| title | Isolation and Genetic Characterization of Parvoviruses From Dogs, Cats, Minks, and Raccoon Dogs in the Eastern Region of Shandong Province, China |
| title_full | Isolation and Genetic Characterization of Parvoviruses From Dogs, Cats, Minks, and Raccoon Dogs in the Eastern Region of Shandong Province, China |
| title_fullStr | Isolation and Genetic Characterization of Parvoviruses From Dogs, Cats, Minks, and Raccoon Dogs in the Eastern Region of Shandong Province, China |
| title_full_unstemmed | Isolation and Genetic Characterization of Parvoviruses From Dogs, Cats, Minks, and Raccoon Dogs in the Eastern Region of Shandong Province, China |
| title_short | Isolation and Genetic Characterization of Parvoviruses From Dogs, Cats, Minks, and Raccoon Dogs in the Eastern Region of Shandong Province, China |
| title_sort | isolation and genetic characterization of parvoviruses from dogs cats minks and raccoon dogs in the eastern region of shandong province china |
| topic | canine parvovirus 2 feline panleukopenia virus genetic characterization mink enteritis virus raccoon dog parvovirus phylogenetic analysis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.862352/full |
| work_keys_str_mv | AT jiakaizhao isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT jiakaizhao isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT haozhang isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT haozhang isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT luzhang isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT luzhang isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT qiangzhang isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT qiangzhang isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT ningzhou isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT ningzhou isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT taofengdu isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT taofengdu isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT qinzhao isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT qinzhao isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT enminzhou isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT enminzhou isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT yongkundu isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT yanisun isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina AT yanisun isolationandgeneticcharacterizationofparvovirusesfromdogscatsminksandraccoondogsintheeasternregionofshandongprovincechina |