Rotaviruses in Wild Ungulates from Germany, 2019–2022
Rotavirus A (RVA) is an important cause of diarrhea in humans and animals. However, RVA in wild animals has only scarcely been investigated so far. Here, the presence of RVA in wild ungulates hunted between 2019 and 2022 in Brandenburg, Germany, was investigated using real-time RT-PCR and sequencing...
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MDPI AG
2023-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/3/566 |
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| author | Nadine Althof Eva Trojnar Reimar Johne |
| author_facet | Nadine Althof Eva Trojnar Reimar Johne |
| author_sort | Nadine Althof |
| collection | DOAJ |
| description | Rotavirus A (RVA) is an important cause of diarrhea in humans and animals. However, RVA in wild animals has only scarcely been investigated so far. Here, the presence of RVA in wild ungulates hunted between 2019 and 2022 in Brandenburg, Germany, was investigated using real-time RT-PCR and sequencing of RT-PCR products. By analyzing intestinal contents, RVA-RNA was detected in 1.0% (2/197) of wild boar (<i>Sus scrofa</i>), 1.3% (2/152) of roe deer (<i>Capreolus capreolus</i>), and 2.1% (2/95) of fallow deer (<i>Dama dama</i>) but not in 28 red deer (<i>Cervus elaphus</i>) samples. Genotyping identified G3P[13] strains in wild boar, which were closely related to previously described pig and wild boar strains. Genotype G10P[15] strains, closely related to strains from roe deer, sheep, or cattle, were found in roe deer. The strains of fallow deer represented genotype G3P[3], clustering in a group containing different strains from several hosts. The results indicated a low prevalence of RVA in wild ungulates in Germany. Associations of specific genotypes with certain ungulate species seem to exist but should be confirmed by analyses of more samples in the future. |
| first_indexed | 2024-03-09T11:00:08Z |
| format | Article |
| id | doaj.art-63b1139fdc1d4b308bf0e8b7d0a80053 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T11:00:08Z |
| publishDate | 2023-02-01 |
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| spelling | doaj.art-63b1139fdc1d4b308bf0e8b7d0a800532023-12-01T01:20:19ZengMDPI AGMicroorganisms2076-26072023-02-0111356610.3390/microorganisms11030566Rotaviruses in Wild Ungulates from Germany, 2019–2022Nadine Althof0Eva Trojnar1Reimar Johne2German Federal Institute for Risk Assessment, Max-Dohrn-Straße 8-10, 10589 Berlin, GermanyGerman Federal Institute for Risk Assessment, Max-Dohrn-Straße 8-10, 10589 Berlin, GermanyGerman Federal Institute for Risk Assessment, Max-Dohrn-Straße 8-10, 10589 Berlin, GermanyRotavirus A (RVA) is an important cause of diarrhea in humans and animals. However, RVA in wild animals has only scarcely been investigated so far. Here, the presence of RVA in wild ungulates hunted between 2019 and 2022 in Brandenburg, Germany, was investigated using real-time RT-PCR and sequencing of RT-PCR products. By analyzing intestinal contents, RVA-RNA was detected in 1.0% (2/197) of wild boar (<i>Sus scrofa</i>), 1.3% (2/152) of roe deer (<i>Capreolus capreolus</i>), and 2.1% (2/95) of fallow deer (<i>Dama dama</i>) but not in 28 red deer (<i>Cervus elaphus</i>) samples. Genotyping identified G3P[13] strains in wild boar, which were closely related to previously described pig and wild boar strains. Genotype G10P[15] strains, closely related to strains from roe deer, sheep, or cattle, were found in roe deer. The strains of fallow deer represented genotype G3P[3], clustering in a group containing different strains from several hosts. The results indicated a low prevalence of RVA in wild ungulates in Germany. Associations of specific genotypes with certain ungulate species seem to exist but should be confirmed by analyses of more samples in the future.https://www.mdpi.com/2076-2607/11/3/566rotavirus Awild boarroe deerfallow deerdetection rategenotyping |
| spellingShingle | Nadine Althof Eva Trojnar Reimar Johne Rotaviruses in Wild Ungulates from Germany, 2019–2022 Microorganisms rotavirus A wild boar roe deer fallow deer detection rate genotyping |
| title | Rotaviruses in Wild Ungulates from Germany, 2019–2022 |
| title_full | Rotaviruses in Wild Ungulates from Germany, 2019–2022 |
| title_fullStr | Rotaviruses in Wild Ungulates from Germany, 2019–2022 |
| title_full_unstemmed | Rotaviruses in Wild Ungulates from Germany, 2019–2022 |
| title_short | Rotaviruses in Wild Ungulates from Germany, 2019–2022 |
| title_sort | rotaviruses in wild ungulates from germany 2019 2022 |
| topic | rotavirus A wild boar roe deer fallow deer detection rate genotyping |
| url | https://www.mdpi.com/2076-2607/11/3/566 |
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