Evaluation of HIF-1α and VEGF-A expression in radiation-induced cystitis: A case-control study
ABSTRACT The standard treatment for locally advanced cervical cancer (CC) is chemoradiotherapy. Once the bladder receives part of the radiation, a typical inflammatory condition that configures radiation-induced cystitis may develop. Chronic radiation-induced cystitis is commonly characterized by th...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Urologia
2021-02-01
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| Series: | International Brazilian Journal of Urology |
| Subjects: | |
| Online Access: | http://www.scielo.br/pdf/ibju/v47n2/1677-6119-ibju-47-02-0295.pdf |
| _version_ | 1819098152665350144 |
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| author | Ricardo Castellani de Mattos Isabella dos Santos Guimarães Leandro de Souza Thiago Andreia Cristina de Melo |
| author_facet | Ricardo Castellani de Mattos Isabella dos Santos Guimarães Leandro de Souza Thiago Andreia Cristina de Melo |
| author_sort | Ricardo Castellani de Mattos |
| collection | DOAJ |
| description | ABSTRACT The standard treatment for locally advanced cervical cancer (CC) is chemoradiotherapy. Once the bladder receives part of the radiation, a typical inflammatory condition that configures radiation-induced cystitis may develop. Chronic radiation-induced cystitis is commonly characterized by the bladder new submucosal vascularization, which is typically fragile and favors hematuria. The current study aims to investigate if Hypoxia-Induced Factor (HIF-1α) and its transcriptional target Vascular Endothelial Growth Factor A (VEGF-A) could be a primary pathway leading to increased submucosal vascularization. HIF-1α and VEGF-A mRNA levels in bladder core biopsies from CC patients treated with radiotherapy versus untreated (non-irradiated) patients were analyzed using a droplet digital polymerase chain reaction technology. Gene expression results showed that HIF-1α and VEGF-A had no significant differences between bladder samples from patients previously irradiated and untreated patient samples. However, a direct relationship between the degree of late morbidity and the expression of HIF-1α and VEGF-A has been demonstrated. Despite the lack of statistical significance precludes a definitive conclusion, the data presented herein suggests that further studies investigating the role of HIF-1α in bladder neovascularization in radiation-induced cystitis are highly recommended. |
| first_indexed | 2024-12-22T00:26:27Z |
| format | Article |
| id | doaj.art-63b316237b1c4b89b5f95c188b4c7868 |
| institution | Directory Open Access Journal |
| issn | 1677-6119 |
| language | English |
| last_indexed | 2024-12-22T00:26:27Z |
| publishDate | 2021-02-01 |
| publisher | Sociedade Brasileira de Urologia |
| record_format | Article |
| series | International Brazilian Journal of Urology |
| spelling | doaj.art-63b316237b1c4b89b5f95c188b4c78682022-12-21T18:45:03ZengSociedade Brasileira de UrologiaInternational Brazilian Journal of Urology1677-61192021-02-0147229530510.1590/s1677-5538.ibju.2020.0054Evaluation of HIF-1α and VEGF-A expression in radiation-induced cystitis: A case-control studyRicardo Castellani de MattosIsabella dos Santos GuimarãesLeandro de Souza Thiagohttps://orcid.org/0000-0002-4003-3400Andreia Cristina de MeloABSTRACT The standard treatment for locally advanced cervical cancer (CC) is chemoradiotherapy. Once the bladder receives part of the radiation, a typical inflammatory condition that configures radiation-induced cystitis may develop. Chronic radiation-induced cystitis is commonly characterized by the bladder new submucosal vascularization, which is typically fragile and favors hematuria. The current study aims to investigate if Hypoxia-Induced Factor (HIF-1α) and its transcriptional target Vascular Endothelial Growth Factor A (VEGF-A) could be a primary pathway leading to increased submucosal vascularization. HIF-1α and VEGF-A mRNA levels in bladder core biopsies from CC patients treated with radiotherapy versus untreated (non-irradiated) patients were analyzed using a droplet digital polymerase chain reaction technology. Gene expression results showed that HIF-1α and VEGF-A had no significant differences between bladder samples from patients previously irradiated and untreated patient samples. However, a direct relationship between the degree of late morbidity and the expression of HIF-1α and VEGF-A has been demonstrated. Despite the lack of statistical significance precludes a definitive conclusion, the data presented herein suggests that further studies investigating the role of HIF-1α in bladder neovascularization in radiation-induced cystitis are highly recommended.http://www.scielo.br/pdf/ibju/v47n2/1677-6119-ibju-47-02-0295.pdfNeoplasmsRadiation-InducedUterine Cervical NeoplasmsVascular Endothelial Growth Factor A |
| spellingShingle | Ricardo Castellani de Mattos Isabella dos Santos Guimarães Leandro de Souza Thiago Andreia Cristina de Melo Evaluation of HIF-1α and VEGF-A expression in radiation-induced cystitis: A case-control study International Brazilian Journal of Urology Neoplasms Radiation-Induced Uterine Cervical Neoplasms Vascular Endothelial Growth Factor A |
| title | Evaluation of HIF-1α and VEGF-A expression in radiation-induced cystitis: A case-control study |
| title_full | Evaluation of HIF-1α and VEGF-A expression in radiation-induced cystitis: A case-control study |
| title_fullStr | Evaluation of HIF-1α and VEGF-A expression in radiation-induced cystitis: A case-control study |
| title_full_unstemmed | Evaluation of HIF-1α and VEGF-A expression in radiation-induced cystitis: A case-control study |
| title_short | Evaluation of HIF-1α and VEGF-A expression in radiation-induced cystitis: A case-control study |
| title_sort | evaluation of hif 1α and vegf a expression in radiation induced cystitis a case control study |
| topic | Neoplasms Radiation-Induced Uterine Cervical Neoplasms Vascular Endothelial Growth Factor A |
| url | http://www.scielo.br/pdf/ibju/v47n2/1677-6119-ibju-47-02-0295.pdf |
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