Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphism
Objective This study was conducted to screen scrotal hernia in domesticated swine from selected breeders in the Philippines. This defect is associated with a cytosine to thymine mutation in the BCL-2 associated X protein (BAX) gene of swine. Methods Genetic screening was done by DNA extraction follo...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2017-02-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-30-2-262.pdf |
| _version_ | 1811334743813259264 |
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| author | Jessica G. Manalaysay Nathaniel D. Antonio Ralph Lorenz R. Apilado Joseph F. Bambico Claro N. Mingala |
| author_facet | Jessica G. Manalaysay Nathaniel D. Antonio Ralph Lorenz R. Apilado Joseph F. Bambico Claro N. Mingala |
| author_sort | Jessica G. Manalaysay |
| collection | DOAJ |
| description | Objective This study was conducted to screen scrotal hernia in domesticated swine from selected breeders in the Philippines. This defect is associated with a cytosine to thymine mutation in the BCL-2 associated X protein (BAX) gene of swine. Methods Genetic screening was done by DNA extraction followed by amplification and digestion using polymerase chain reaction-restriction fragment length polymorphism, amplifying the 416 bp region of the BAX gene that was subjected to digestion using the Ear I enzyme. Sequencing was also conducted to validate the results. Results Results revealed that out of 538 samples tested, 411 (76.4%) of the samples were found to be normal whereas the remaining were carriers of the mutation in which 80 (14.9%) were heterozygous mutants and 47 (8.7%) were homozygous mutants. Pietrain breed was found to have the highest incidence. Conclusion Having a scrotal hernia eliminates the chances of using the boar as a breeder stock because the following generations arising from it would most likely exhibit herniation. It is therefore advised to establish a genetic screening method for Scrotal Hernia in the Philippines to eliminate the negative gene from the herd. |
| first_indexed | 2024-04-13T17:13:54Z |
| format | Article |
| id | doaj.art-63b34a53b91247f4b218c88d431700fc |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-04-13T17:13:54Z |
| publishDate | 2017-02-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-63b34a53b91247f4b218c88d431700fc2022-12-22T02:38:12ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172017-02-0130226226610.5713/ajas.16.002223537Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphismJessica G. Manalaysay0Nathaniel D. Antonio1Ralph Lorenz R. Apilado2Joseph F. Bambico3Claro N. Mingala4 Philippine Carabao Center, Science City of Munoz, Nueva Ecija 3120, Philippines College of Science, University of the Philippines Baguio, Baguio2600, Philippines College of Science, University of the Philippines Baguio, Baguio2600, Philippines College of Science, University of the Philippines Baguio, Baguio2600, Philippines Philippine Carabao Center, Science City of Munoz, Nueva Ecija 3120, PhilippinesObjective This study was conducted to screen scrotal hernia in domesticated swine from selected breeders in the Philippines. This defect is associated with a cytosine to thymine mutation in the BCL-2 associated X protein (BAX) gene of swine. Methods Genetic screening was done by DNA extraction followed by amplification and digestion using polymerase chain reaction-restriction fragment length polymorphism, amplifying the 416 bp region of the BAX gene that was subjected to digestion using the Ear I enzyme. Sequencing was also conducted to validate the results. Results Results revealed that out of 538 samples tested, 411 (76.4%) of the samples were found to be normal whereas the remaining were carriers of the mutation in which 80 (14.9%) were heterozygous mutants and 47 (8.7%) were homozygous mutants. Pietrain breed was found to have the highest incidence. Conclusion Having a scrotal hernia eliminates the chances of using the boar as a breeder stock because the following generations arising from it would most likely exhibit herniation. It is therefore advised to establish a genetic screening method for Scrotal Hernia in the Philippines to eliminate the negative gene from the herd.http://www.ajas.info/upload/pdf/ajas-30-2-262.pdf GenePolymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP)ScreeningScrotal HerniaSwine |
| spellingShingle | Jessica G. Manalaysay Nathaniel D. Antonio Ralph Lorenz R. Apilado Joseph F. Bambico Claro N. Mingala Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphism Asian-Australasian Journal of Animal Sciences Gene Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Screening Scrotal Hernia Swine |
| title | Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphism |
| title_full | Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphism |
| title_fullStr | Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphism |
| title_full_unstemmed | Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphism |
| title_short | Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphism |
| title_sort | screening of bcl 2 associated x protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction restriction fragment length polymorphism |
| topic | Gene Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Screening Scrotal Hernia Swine |
| url | http://www.ajas.info/upload/pdf/ajas-30-2-262.pdf |
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