Thin-layer chromatography: An efficient method for the optimization of dispersive liquid-liquid microextraction
Thin-layer chromatography (TLC) is an often omitted analytical technique due to its lower sensitivity and separation capacity. Even in the era of high-performance liquid chromatography (HPLC), thin-layer chromatography still offers many advantages, such as simplicity, rapidity, and cost-effectivenes...
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| Format: | Article |
| Language: | English |
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Slovenian Chemical Society
2018-06-01
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| Series: | Acta Chimica Slovenica |
| Subjects: | |
| Online Access: | https://journals.matheo.si/index.php/ACSi/article/view/4108 |
| _version_ | 1818303467257593856 |
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| author | Elena Kupcova Katarína Reiffová Yaroslav Bazeľ |
| author_facet | Elena Kupcova Katarína Reiffová Yaroslav Bazeľ |
| author_sort | Elena Kupcova |
| collection | DOAJ |
| description | Thin-layer chromatography (TLC) is an often omitted analytical technique due to its lower sensitivity and separation capacity. Even in the era of high-performance liquid chromatography (HPLC), thin-layer chromatography still offers many advantages, such as simplicity, rapidity, and cost-effectiveness, which predict TLC to be the first-choice method for the laborious optimization process requiring analysis of numerous samples. In this work, a thin-layer chromatography method with chemical and densitometric detection was used to optimize a dispersive liquid-liquid microextraction (DLLME) process for the extraction and preconcentration of estradiol in human urine. The chromatographic system consisted of silica gel plates as the stationary phase and toluene-ethanol (9:1; v/v) mixture as the developing solvent. The plates were dyed with 10% phosphomolybdic acid reagent and sequentially evaluated densitometrically at λ = 430 nm. In the context of DLLME optimization, parameters including the type and volume of extraction and dispersive solvents, centrifugation, salt addition and extraction time, were studied. The proposed DLLME-TLC method was successfully applied to the determination of estradiol in real human urine samples. |
| first_indexed | 2024-12-13T05:55:16Z |
| format | Article |
| id | doaj.art-63b3c3d3f09c4960b20e446fa2d63a71 |
| institution | Directory Open Access Journal |
| issn | 1318-0207 1580-3155 |
| language | English |
| last_indexed | 2024-12-13T05:55:16Z |
| publishDate | 2018-06-01 |
| publisher | Slovenian Chemical Society |
| record_format | Article |
| series | Acta Chimica Slovenica |
| spelling | doaj.art-63b3c3d3f09c4960b20e446fa2d63a712022-12-21T23:57:26ZengSlovenian Chemical SocietyActa Chimica Slovenica1318-02071580-31552018-06-0165238839310.17344/acsi.2017.4108584Thin-layer chromatography: An efficient method for the optimization of dispersive liquid-liquid microextractionElena Kupcova0Katarína Reiffová1Yaroslav Bazeľ2Department of Analytical Chemistry, Faculty of Science, Pavol Jozef Safarik University in KosiceDepartment of Analytical Chemistry, Faculty of Science, Pavol Jozef Safarik University in KosiceDepartment of Analytical Chemistry, Faculty of Science, Pavol Jozef Safarik University in KosiceThin-layer chromatography (TLC) is an often omitted analytical technique due to its lower sensitivity and separation capacity. Even in the era of high-performance liquid chromatography (HPLC), thin-layer chromatography still offers many advantages, such as simplicity, rapidity, and cost-effectiveness, which predict TLC to be the first-choice method for the laborious optimization process requiring analysis of numerous samples. In this work, a thin-layer chromatography method with chemical and densitometric detection was used to optimize a dispersive liquid-liquid microextraction (DLLME) process for the extraction and preconcentration of estradiol in human urine. The chromatographic system consisted of silica gel plates as the stationary phase and toluene-ethanol (9:1; v/v) mixture as the developing solvent. The plates were dyed with 10% phosphomolybdic acid reagent and sequentially evaluated densitometrically at λ = 430 nm. In the context of DLLME optimization, parameters including the type and volume of extraction and dispersive solvents, centrifugation, salt addition and extraction time, were studied. The proposed DLLME-TLC method was successfully applied to the determination of estradiol in real human urine samples.https://journals.matheo.si/index.php/ACSi/article/view/4108thin-layer chromatographypostchromatographic detectiondispersive liquid-liquid microextractionestradiolhuman urine |
| spellingShingle | Elena Kupcova Katarína Reiffová Yaroslav Bazeľ Thin-layer chromatography: An efficient method for the optimization of dispersive liquid-liquid microextraction Acta Chimica Slovenica thin-layer chromatography postchromatographic detection dispersive liquid-liquid microextraction estradiol human urine |
| title | Thin-layer chromatography: An efficient method for the optimization of dispersive liquid-liquid microextraction |
| title_full | Thin-layer chromatography: An efficient method for the optimization of dispersive liquid-liquid microextraction |
| title_fullStr | Thin-layer chromatography: An efficient method for the optimization of dispersive liquid-liquid microextraction |
| title_full_unstemmed | Thin-layer chromatography: An efficient method for the optimization of dispersive liquid-liquid microextraction |
| title_short | Thin-layer chromatography: An efficient method for the optimization of dispersive liquid-liquid microextraction |
| title_sort | thin layer chromatography an efficient method for the optimization of dispersive liquid liquid microextraction |
| topic | thin-layer chromatography postchromatographic detection dispersive liquid-liquid microextraction estradiol human urine |
| url | https://journals.matheo.si/index.php/ACSi/article/view/4108 |
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