Antioxidant and Anti-Inflammatory Properties of Recombinant <i>Bifidobacterium bifidum</i> BGN4 Expressing Antioxidant Enzymes
<i>Bifidobacterium bifidum</i> BGN4-SK (BGN4-SK), a recombinant strain which was constructed from <i>B. bifidum</i> BGN4 (BGN4) to produce superoxide dismutase (SOD) and catalase, was analyzed to determine its antioxidant and anti-inflammatory properties in vitro. Culture con...
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MDPI AG
2021-03-01
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| Online Access: | https://www.mdpi.com/2076-2607/9/3/595 |
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| author | Zhaoyan Lin Seockmo Ku Taehwan Lim Sun Young Park Myeong Soo Park Geun Eog Ji Keely O’Brien Keum Taek Hwang |
| author_facet | Zhaoyan Lin Seockmo Ku Taehwan Lim Sun Young Park Myeong Soo Park Geun Eog Ji Keely O’Brien Keum Taek Hwang |
| author_sort | Zhaoyan Lin |
| collection | DOAJ |
| description | <i>Bifidobacterium bifidum</i> BGN4-SK (BGN4-SK), a recombinant strain which was constructed from <i>B. bifidum</i> BGN4 (BGN4) to produce superoxide dismutase (SOD) and catalase, was analyzed to determine its antioxidant and anti-inflammatory properties in vitro. Culture conditions were determined to maximize the SOD and catalase activities of BGN4-SK. The viability, intracellular radical oxygen species (ROS) levels, intracellular antioxidant enzyme activities, and pro-inflammatory cytokine levels were determined to evaluate the antioxidant and anti-inflammatory activities of BGN4-SK in human intestinal epithelial cells (HT-29) and murine macrophage cells (RAW 264.7). Antioxidant enzymes (SOD and catalase) were produced at the highest levels when BGN4-SK was cultured for 24 h in a medium containing 500 μM MnSO<sub>4</sub> and 30 μM hematin, with glucose as the carbon source. The viability and intracellular antioxidant enzyme activities of H<sub>2</sub>O<sub>2</sub>-stimulated HT-29 treated with BGN4-SK were significantly higher (<i>p</i> < 0.05) than those of cells treated with BGN4. The intracellular ROS levels of H<sub>2</sub>O<sub>2</sub>-stimulated HT-29 cells treated with BGN4-SK were significantly lower (<i>p</i> < 0.05) than those of cells treated with BGN4. BGN4-SK more significantly suppressed the production of interleukin (IL)-6 (<i>p</i> < 0.05), tumor necrosis factor-α (<i>p</i> < 0.01), and IL-8 (<i>p</i> < 0.05) in lipopolysaccharide (LPS)-stimulated HT-29 and LPS-stimulated RAW 264.7 cells compared to BGN4. These results suggest that BGN4-SK may have enhanced antioxidant activities against oxidative stress in H<sub>2</sub>O<sub>2</sub>-stimulated HT-29 cells and enhanced anti-inflammatory activities in LPS-stimulated HT-29 and RAW 264.7 cells. |
| first_indexed | 2024-03-10T13:15:23Z |
| format | Article |
| id | doaj.art-63b539844c70425aba5d5a6a47e420f8 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T13:15:23Z |
| publishDate | 2021-03-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-63b539844c70425aba5d5a6a47e420f82023-11-21T10:24:24ZengMDPI AGMicroorganisms2076-26072021-03-019359510.3390/microorganisms9030595Antioxidant and Anti-Inflammatory Properties of Recombinant <i>Bifidobacterium bifidum</i> BGN4 Expressing Antioxidant EnzymesZhaoyan Lin0Seockmo Ku1Taehwan Lim2Sun Young Park3Myeong Soo Park4Geun Eog Ji5Keely O’Brien6Keum Taek Hwang7Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University, Seoul 08826, KoreaFermentation Science Program, School of Agriculture, College of Basic and Applied Sciences, Middle Tennessee State University, Murfreesboro, TN 37132, USADepartment of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University, Seoul 08826, KoreaDepartment of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University, Seoul 08826, KoreaResearch Center, BIFIDO Co., Ltd., Hongcheon 25117, KoreaResearch Center, BIFIDO Co., Ltd., Hongcheon 25117, KoreaFermentation Science Program, School of Agriculture, College of Basic and Applied Sciences, Middle Tennessee State University, Murfreesboro, TN 37132, USADepartment of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University, Seoul 08826, Korea<i>Bifidobacterium bifidum</i> BGN4-SK (BGN4-SK), a recombinant strain which was constructed from <i>B. bifidum</i> BGN4 (BGN4) to produce superoxide dismutase (SOD) and catalase, was analyzed to determine its antioxidant and anti-inflammatory properties in vitro. Culture conditions were determined to maximize the SOD and catalase activities of BGN4-SK. The viability, intracellular radical oxygen species (ROS) levels, intracellular antioxidant enzyme activities, and pro-inflammatory cytokine levels were determined to evaluate the antioxidant and anti-inflammatory activities of BGN4-SK in human intestinal epithelial cells (HT-29) and murine macrophage cells (RAW 264.7). Antioxidant enzymes (SOD and catalase) were produced at the highest levels when BGN4-SK was cultured for 24 h in a medium containing 500 μM MnSO<sub>4</sub> and 30 μM hematin, with glucose as the carbon source. The viability and intracellular antioxidant enzyme activities of H<sub>2</sub>O<sub>2</sub>-stimulated HT-29 treated with BGN4-SK were significantly higher (<i>p</i> < 0.05) than those of cells treated with BGN4. The intracellular ROS levels of H<sub>2</sub>O<sub>2</sub>-stimulated HT-29 cells treated with BGN4-SK were significantly lower (<i>p</i> < 0.05) than those of cells treated with BGN4. BGN4-SK more significantly suppressed the production of interleukin (IL)-6 (<i>p</i> < 0.05), tumor necrosis factor-α (<i>p</i> < 0.01), and IL-8 (<i>p</i> < 0.05) in lipopolysaccharide (LPS)-stimulated HT-29 and LPS-stimulated RAW 264.7 cells compared to BGN4. These results suggest that BGN4-SK may have enhanced antioxidant activities against oxidative stress in H<sub>2</sub>O<sub>2</sub>-stimulated HT-29 cells and enhanced anti-inflammatory activities in LPS-stimulated HT-29 and RAW 264.7 cells.https://www.mdpi.com/2076-2607/9/3/595recombinant bifidobacteriacatalasesuperoxide peroxidase |
| spellingShingle | Zhaoyan Lin Seockmo Ku Taehwan Lim Sun Young Park Myeong Soo Park Geun Eog Ji Keely O’Brien Keum Taek Hwang Antioxidant and Anti-Inflammatory Properties of Recombinant <i>Bifidobacterium bifidum</i> BGN4 Expressing Antioxidant Enzymes Microorganisms recombinant bifidobacteria catalase superoxide peroxidase |
| title | Antioxidant and Anti-Inflammatory Properties of Recombinant <i>Bifidobacterium bifidum</i> BGN4 Expressing Antioxidant Enzymes |
| title_full | Antioxidant and Anti-Inflammatory Properties of Recombinant <i>Bifidobacterium bifidum</i> BGN4 Expressing Antioxidant Enzymes |
| title_fullStr | Antioxidant and Anti-Inflammatory Properties of Recombinant <i>Bifidobacterium bifidum</i> BGN4 Expressing Antioxidant Enzymes |
| title_full_unstemmed | Antioxidant and Anti-Inflammatory Properties of Recombinant <i>Bifidobacterium bifidum</i> BGN4 Expressing Antioxidant Enzymes |
| title_short | Antioxidant and Anti-Inflammatory Properties of Recombinant <i>Bifidobacterium bifidum</i> BGN4 Expressing Antioxidant Enzymes |
| title_sort | antioxidant and anti inflammatory properties of recombinant i bifidobacterium bifidum i bgn4 expressing antioxidant enzymes |
| topic | recombinant bifidobacteria catalase superoxide peroxidase |
| url | https://www.mdpi.com/2076-2607/9/3/595 |
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