Investigating the Ability of <i>Edwardsiella ictaluri</i> and <i>Flavobacterium covae</i> to Persist within Commercial Catfish Pond Sediments under Laboratory Conditions
Two prevalent bacterial diseases in catfish aquaculture are enteric septicemia of catfish and columnaris disease caused by <i>Edwardsiella ictaluri</i> and <i>Flavobacterium covae</i>, respectively. Chronic and recurring outbreaks of these bacterial pathogens result in signif...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/7/871 |
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| author | James T. Tuttle Timothy J. Bruce Ian A. E. Butts Luke A. Roy Hisham A. Abdelrahman Benjamin H. Beck Anita M. Kelly |
| author_facet | James T. Tuttle Timothy J. Bruce Ian A. E. Butts Luke A. Roy Hisham A. Abdelrahman Benjamin H. Beck Anita M. Kelly |
| author_sort | James T. Tuttle |
| collection | DOAJ |
| description | Two prevalent bacterial diseases in catfish aquaculture are enteric septicemia of catfish and columnaris disease caused by <i>Edwardsiella ictaluri</i> and <i>Flavobacterium covae</i>, respectively. Chronic and recurring outbreaks of these bacterial pathogens result in significant economic losses for producers annually. Determining if these pathogens can persist within sediments of commercial ponds is paramount. Experimental persistence trials (PT) were conducted to evaluate the persistence of <i>E. ictaluri</i> and <i>F. covae</i> in pond sediments. Twelve test chambers containing 120 g of sterilized sediment from four commercial catfish ponds were inoculated with either <i>E. ictaluri</i> (S97-773) or <i>F. covae</i> (ALG-00-530) and filled with 8 L of disinfected water. At 1, 2, 4-, 6-, 8-, and 15-days post-inoculation, 1 g of sediment was removed, and colony-forming units (CFU) were enumerated on selective media using 6 × 6 drop plate methods. <i>E. ictaluri</i> population peaked on Day 3 at 6.4 ± 0.5 log<sub>10</sub> CFU g<sup>−1</sup>. Correlation analysis revealed no correlation between the sediment physicochemical parameters and <i>E. ictaluri</i> log<sub>10</sub> CFU g<sup>−1</sup>. However, no viable <i>F. covae</i> colonies were recovered after two PT attempts. Future studies to improve understanding of <i>E. ictaluri</i> pathogenesis and persistence, and potential <i>F. covae</i> persistence in pond bottom sediments are needed. |
| first_indexed | 2024-03-11T00:44:49Z |
| format | Article |
| id | doaj.art-63b734b63faf4ba9aae716a43c13fa02 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-11T00:44:49Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-63b734b63faf4ba9aae716a43c13fa022023-11-18T20:50:10ZengMDPI AGPathogens2076-08172023-06-0112787110.3390/pathogens12070871Investigating the Ability of <i>Edwardsiella ictaluri</i> and <i>Flavobacterium covae</i> to Persist within Commercial Catfish Pond Sediments under Laboratory ConditionsJames T. Tuttle0Timothy J. Bruce1Ian A. E. Butts2Luke A. Roy3Hisham A. Abdelrahman4Benjamin H. Beck5Anita M. Kelly6Alabama Fish Farming Center, Greensboro, AL 36744, USASchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL 36849, USASchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL 36849, USAAlabama Fish Farming Center, Greensboro, AL 36744, USAAlabama Fish Farming Center, Greensboro, AL 36744, USAAquatic Animal Health Research Unit, US Department of Agriculture, Agricultural Research Service, Auburn, AL 36832, USAAlabama Fish Farming Center, Greensboro, AL 36744, USATwo prevalent bacterial diseases in catfish aquaculture are enteric septicemia of catfish and columnaris disease caused by <i>Edwardsiella ictaluri</i> and <i>Flavobacterium covae</i>, respectively. Chronic and recurring outbreaks of these bacterial pathogens result in significant economic losses for producers annually. Determining if these pathogens can persist within sediments of commercial ponds is paramount. Experimental persistence trials (PT) were conducted to evaluate the persistence of <i>E. ictaluri</i> and <i>F. covae</i> in pond sediments. Twelve test chambers containing 120 g of sterilized sediment from four commercial catfish ponds were inoculated with either <i>E. ictaluri</i> (S97-773) or <i>F. covae</i> (ALG-00-530) and filled with 8 L of disinfected water. At 1, 2, 4-, 6-, 8-, and 15-days post-inoculation, 1 g of sediment was removed, and colony-forming units (CFU) were enumerated on selective media using 6 × 6 drop plate methods. <i>E. ictaluri</i> population peaked on Day 3 at 6.4 ± 0.5 log<sub>10</sub> CFU g<sup>−1</sup>. Correlation analysis revealed no correlation between the sediment physicochemical parameters and <i>E. ictaluri</i> log<sub>10</sub> CFU g<sup>−1</sup>. However, no viable <i>F. covae</i> colonies were recovered after two PT attempts. Future studies to improve understanding of <i>E. ictaluri</i> pathogenesis and persistence, and potential <i>F. covae</i> persistence in pond bottom sediments are needed.https://www.mdpi.com/2076-0817/12/7/871bacterial diseasescatfishcommercial aquaculturesoil microbiologyenvironmental adaptations |
| spellingShingle | James T. Tuttle Timothy J. Bruce Ian A. E. Butts Luke A. Roy Hisham A. Abdelrahman Benjamin H. Beck Anita M. Kelly Investigating the Ability of <i>Edwardsiella ictaluri</i> and <i>Flavobacterium covae</i> to Persist within Commercial Catfish Pond Sediments under Laboratory Conditions Pathogens bacterial diseases catfish commercial aquaculture soil microbiology environmental adaptations |
| title | Investigating the Ability of <i>Edwardsiella ictaluri</i> and <i>Flavobacterium covae</i> to Persist within Commercial Catfish Pond Sediments under Laboratory Conditions |
| title_full | Investigating the Ability of <i>Edwardsiella ictaluri</i> and <i>Flavobacterium covae</i> to Persist within Commercial Catfish Pond Sediments under Laboratory Conditions |
| title_fullStr | Investigating the Ability of <i>Edwardsiella ictaluri</i> and <i>Flavobacterium covae</i> to Persist within Commercial Catfish Pond Sediments under Laboratory Conditions |
| title_full_unstemmed | Investigating the Ability of <i>Edwardsiella ictaluri</i> and <i>Flavobacterium covae</i> to Persist within Commercial Catfish Pond Sediments under Laboratory Conditions |
| title_short | Investigating the Ability of <i>Edwardsiella ictaluri</i> and <i>Flavobacterium covae</i> to Persist within Commercial Catfish Pond Sediments under Laboratory Conditions |
| title_sort | investigating the ability of i edwardsiella ictaluri i and i flavobacterium covae i to persist within commercial catfish pond sediments under laboratory conditions |
| topic | bacterial diseases catfish commercial aquaculture soil microbiology environmental adaptations |
| url | https://www.mdpi.com/2076-0817/12/7/871 |
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