Combined Effects of Carotenoids and Polyphenols in Balancing the Response of Skin Cells to UV Irradiation
Oral carotenoids and polyphenols have been suggested to induce photo-protective effects. The aim of the study was to test whether the combination of carotenoids and polyphenols produce greater protective effects from UV-induced damage to skin cells. Such damage is characterized by inflammation and o...
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MDPI AG
2021-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/7/1931 |
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| author | Glenda Calniquer Marina Khanin Hilla Ovadia Karin Linnewiel-Hermoni David Stepensky Aviram Trachtenberg Tanya Sedlov Oleg Braverman Joseph Levy Yoav Sharoni |
| author_facet | Glenda Calniquer Marina Khanin Hilla Ovadia Karin Linnewiel-Hermoni David Stepensky Aviram Trachtenberg Tanya Sedlov Oleg Braverman Joseph Levy Yoav Sharoni |
| author_sort | Glenda Calniquer |
| collection | DOAJ |
| description | Oral carotenoids and polyphenols have been suggested to induce photo-protective effects. The aim of the study was to test whether the combination of carotenoids and polyphenols produce greater protective effects from UV-induced damage to skin cells. Such damage is characterized by inflammation and oxidative stress; thus, the photo-protective effect can be partially explained by modulating the nuclear factor kappa B (NFκB) and antioxidant response element/Nrf2 (ARE/Nrf2) transcription systems, known as important regulators of these two processes. Indeed, it was found in keratinocytes that carotenoids and polyphenols inhibit UVB-induced NFκB activity and release of cytokine IL-6. A combination of tomato extract with rosemary extract inhibited UVB-induced release of IL-6 more than each of the compounds alone. Moreover, this combination synergistically activated ARE/Nrf2 transcription systems. Inflammatory cytokines such as IL-6 and TNFα induce the expression of matrix metalloproteinases (MMPs), which leads to collagen breakdown; thus, it is important to note that carnosic acid reduced TNFα-induced MMP-1 secretion from human dermal fibroblasts. The in vitro results suggest beneficial effects of phytonutrient combinations on skin health. To assure that clinical experiments to prove such effects in humans are feasible, the human bioavailability of carotenoids from tomato extract was tested, and nearly a twofold increase in their plasma concentrations was detected. This study demonstrates that carotenoids and polyphenols cooperate in balancing UV-induced skin cell damage, and suggests that NFκB and ARE/Nrf2 are involved in these effects. |
| first_indexed | 2024-03-10T12:47:23Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T12:47:23Z |
| publishDate | 2021-03-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-63bc52356fa44b9c949a41d574b27fba2023-11-21T13:22:18ZengMDPI AGMolecules1420-30492021-03-01267193110.3390/molecules26071931Combined Effects of Carotenoids and Polyphenols in Balancing the Response of Skin Cells to UV IrradiationGlenda Calniquer0Marina Khanin1Hilla Ovadia2Karin Linnewiel-Hermoni3David Stepensky4Aviram Trachtenberg5Tanya Sedlov6Oleg Braverman7Joseph Levy8Yoav Sharoni9Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelDepartment of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelDepartment of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelLycored, Secaucus, NJ 07094, USADepartment of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelDepartment of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelLycored Ltd., Hebron Rd. P.O.B. 320, Beer Sheva 84102, IsraelLycored Ltd., Hebron Rd. P.O.B. 320, Beer Sheva 84102, IsraelDepartment of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelDepartment of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelOral carotenoids and polyphenols have been suggested to induce photo-protective effects. The aim of the study was to test whether the combination of carotenoids and polyphenols produce greater protective effects from UV-induced damage to skin cells. Such damage is characterized by inflammation and oxidative stress; thus, the photo-protective effect can be partially explained by modulating the nuclear factor kappa B (NFκB) and antioxidant response element/Nrf2 (ARE/Nrf2) transcription systems, known as important regulators of these two processes. Indeed, it was found in keratinocytes that carotenoids and polyphenols inhibit UVB-induced NFκB activity and release of cytokine IL-6. A combination of tomato extract with rosemary extract inhibited UVB-induced release of IL-6 more than each of the compounds alone. Moreover, this combination synergistically activated ARE/Nrf2 transcription systems. Inflammatory cytokines such as IL-6 and TNFα induce the expression of matrix metalloproteinases (MMPs), which leads to collagen breakdown; thus, it is important to note that carnosic acid reduced TNFα-induced MMP-1 secretion from human dermal fibroblasts. The in vitro results suggest beneficial effects of phytonutrient combinations on skin health. To assure that clinical experiments to prove such effects in humans are feasible, the human bioavailability of carotenoids from tomato extract was tested, and nearly a twofold increase in their plasma concentrations was detected. This study demonstrates that carotenoids and polyphenols cooperate in balancing UV-induced skin cell damage, and suggests that NFκB and ARE/Nrf2 are involved in these effects.https://www.mdpi.com/1420-3049/26/7/1931lycopenecarnosic acidkeratinocytesinterleukin-6antioxidant response element/Nrf2NFκB |
| spellingShingle | Glenda Calniquer Marina Khanin Hilla Ovadia Karin Linnewiel-Hermoni David Stepensky Aviram Trachtenberg Tanya Sedlov Oleg Braverman Joseph Levy Yoav Sharoni Combined Effects of Carotenoids and Polyphenols in Balancing the Response of Skin Cells to UV Irradiation Molecules lycopene carnosic acid keratinocytes interleukin-6 antioxidant response element/Nrf2 NFκB |
| title | Combined Effects of Carotenoids and Polyphenols in Balancing the Response of Skin Cells to UV Irradiation |
| title_full | Combined Effects of Carotenoids and Polyphenols in Balancing the Response of Skin Cells to UV Irradiation |
| title_fullStr | Combined Effects of Carotenoids and Polyphenols in Balancing the Response of Skin Cells to UV Irradiation |
| title_full_unstemmed | Combined Effects of Carotenoids and Polyphenols in Balancing the Response of Skin Cells to UV Irradiation |
| title_short | Combined Effects of Carotenoids and Polyphenols in Balancing the Response of Skin Cells to UV Irradiation |
| title_sort | combined effects of carotenoids and polyphenols in balancing the response of skin cells to uv irradiation |
| topic | lycopene carnosic acid keratinocytes interleukin-6 antioxidant response element/Nrf2 NFκB |
| url | https://www.mdpi.com/1420-3049/26/7/1931 |
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