β‐asarone inhibits the migration, invasion, and EMT of bladder cancer through activating ER stress
Abstract Background β‐asarone (β‐as), a compound extracted from Acorus calamus, has been found to have anticancer effects on a variety of human cancers. However, the potential effect of β‐as on bladder cancer (BCa) remains unknown. Methods After exposure to β‐as, migration, invasion, and epithelial‐...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-06-01
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| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.6059 |
| _version_ | 1827896599084269568 |
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| author | Bo Liu Weichao Dan Yi Wei Yan Zhang Chi Wang Yuzeshi Lei Tao Hou Yong Zhang Ye Gao |
| author_facet | Bo Liu Weichao Dan Yi Wei Yan Zhang Chi Wang Yuzeshi Lei Tao Hou Yong Zhang Ye Gao |
| author_sort | Bo Liu |
| collection | DOAJ |
| description | Abstract Background β‐asarone (β‐as), a compound extracted from Acorus calamus, has been found to have anticancer effects on a variety of human cancers. However, the potential effect of β‐as on bladder cancer (BCa) remains unknown. Methods After exposure to β‐as, migration, invasion, and epithelial‐mesenchymal transition (EMT) of BCa were determined by wound healing, transwell, and Western blot assays. Expression of proteins involved in the EMT and ER stress were explored by Western blot assays. Nude mouse xenograft model was served as the model system in vivo. Results The migration, invasion, and EMT of BCa were significantly inhibited after β‐as treatment. Further experiments revealed that endoplasmic reticulum (ER) stress is involved in β‐as‐mediated metastasis inhibition. In addition, β‐as significantly up‐regulated activating transcription factor 6 (ATF6), a branch of ER stress, and promoted its Golgi cleavage and nuclear localization. ATF6 silencing attenuated β‐as‐mediated metastasis and EMT inhibition in BCa cells. Conclusion Our data suggests that β‐as inhibits migration, invasion, and EMT of BCa by activating the ATF6 branch of ER stress. Thus, β‐as represents a potential candidate for BCa treatment. |
| first_indexed | 2024-03-12T22:38:27Z |
| format | Article |
| id | doaj.art-63bd67a6081a447b84c21d7783d58c3e |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-03-12T22:38:27Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-63bd67a6081a447b84c21d7783d58c3e2023-07-21T11:20:58ZengWileyCancer Medicine2045-76342023-06-011212136101362210.1002/cam4.6059β‐asarone inhibits the migration, invasion, and EMT of bladder cancer through activating ER stressBo Liu0Weichao Dan1Yi Wei2Yan Zhang3Chi Wang4Yuzeshi Lei5Tao Hou6Yong Zhang7Ye Gao8Department of Surgical Oncology The First Affiliated Hospital of Xi'an Jiaotong University Xi'an P. R. ChinaDepartment of Urology The First Affiliated Hospital of Xi'an Jiaotong University Xi'an P. R. ChinaDepartment of Urology The First Affiliated Hospital of Xi'an Jiaotong University Xi'an P. R. ChinaDepartment of Surgical Oncology The First Affiliated Hospital of Xi'an Jiaotong University Xi'an P. R. ChinaDepartment of Urology The First Affiliated Hospital of Xi'an Jiaotong University Xi'an P. R. ChinaDepartment of Urology The First Affiliated Hospital of Xi'an Jiaotong University Xi'an P. R. ChinaDepartment of Urology The First Affiliated Hospital of Xi'an Jiaotong University Xi'an P. R. ChinaDepartment of Surgical Oncology The First Affiliated Hospital of Xi'an Jiaotong University Xi'an P. R. ChinaDepartment of Emergency The First Affiliated Hospital of Xi'an Jiaotong University Xi'an P. R. ChinaAbstract Background β‐asarone (β‐as), a compound extracted from Acorus calamus, has been found to have anticancer effects on a variety of human cancers. However, the potential effect of β‐as on bladder cancer (BCa) remains unknown. Methods After exposure to β‐as, migration, invasion, and epithelial‐mesenchymal transition (EMT) of BCa were determined by wound healing, transwell, and Western blot assays. Expression of proteins involved in the EMT and ER stress were explored by Western blot assays. Nude mouse xenograft model was served as the model system in vivo. Results The migration, invasion, and EMT of BCa were significantly inhibited after β‐as treatment. Further experiments revealed that endoplasmic reticulum (ER) stress is involved in β‐as‐mediated metastasis inhibition. In addition, β‐as significantly up‐regulated activating transcription factor 6 (ATF6), a branch of ER stress, and promoted its Golgi cleavage and nuclear localization. ATF6 silencing attenuated β‐as‐mediated metastasis and EMT inhibition in BCa cells. Conclusion Our data suggests that β‐as inhibits migration, invasion, and EMT of BCa by activating the ATF6 branch of ER stress. Thus, β‐as represents a potential candidate for BCa treatment.https://doi.org/10.1002/cam4.6059ATF6EMTER stressinvasionmigrationβ‐asarone |
| spellingShingle | Bo Liu Weichao Dan Yi Wei Yan Zhang Chi Wang Yuzeshi Lei Tao Hou Yong Zhang Ye Gao β‐asarone inhibits the migration, invasion, and EMT of bladder cancer through activating ER stress Cancer Medicine ATF6 EMT ER stress invasion migration β‐asarone |
| title | β‐asarone inhibits the migration, invasion, and EMT of bladder cancer through activating ER stress |
| title_full | β‐asarone inhibits the migration, invasion, and EMT of bladder cancer through activating ER stress |
| title_fullStr | β‐asarone inhibits the migration, invasion, and EMT of bladder cancer through activating ER stress |
| title_full_unstemmed | β‐asarone inhibits the migration, invasion, and EMT of bladder cancer through activating ER stress |
| title_short | β‐asarone inhibits the migration, invasion, and EMT of bladder cancer through activating ER stress |
| title_sort | β asarone inhibits the migration invasion and emt of bladder cancer through activating er stress |
| topic | ATF6 EMT ER stress invasion migration β‐asarone |
| url | https://doi.org/10.1002/cam4.6059 |
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