Microscopic characterization reveals the diversity of EVs secreted by GFP-HAS3 expressing MCF7 cells
We have shown the connection of hyaluronan synthesis activity with the enhanced shedding of extracellular vesicles, but detailed morphological analysis of those hyaluronan-induced EVs is still missing. In this study we utilized a comprehensive set of high-resolution imaging techniques to characteriz...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | European Journal of Cell Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171933522000383 |
| _version_ | 1811315776468025344 |
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| author | Janne Capra Kai Härkönen Heikki Kyykallio Helena Vihinen Eija Jokitalo Kirsi Rilla |
| author_facet | Janne Capra Kai Härkönen Heikki Kyykallio Helena Vihinen Eija Jokitalo Kirsi Rilla |
| author_sort | Janne Capra |
| collection | DOAJ |
| description | We have shown the connection of hyaluronan synthesis activity with the enhanced shedding of extracellular vesicles, but detailed morphological analysis of those hyaluronan-induced EVs is still missing. In this study we utilized a comprehensive set of high-resolution imaging techniques to characterize in high detail the size and morphology of EVs originating from stable MCF7 breast cancer cell line and transiently transfected cells expressing GFP-HAS3. To avoid possible artefacts or loss of EVs resulting from the isolation process, special attention was paid to analysis of EVs in situ in monolayer and in 3D cultures. The results of this study show that GFP-HAS3 expressing MCF7 cells produce morphologically diverse EVs but also demonstrates the variation in results obtained with different experimental setup, which emphasizes the importance of comparison between different methods when interpreting the observations. |
| first_indexed | 2024-04-13T11:37:22Z |
| format | Article |
| id | doaj.art-63c0cded6bc1489d8cfb1f119d34f175 |
| institution | Directory Open Access Journal |
| issn | 0171-9335 |
| language | English |
| last_indexed | 2024-04-13T11:37:22Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | European Journal of Cell Biology |
| spelling | doaj.art-63c0cded6bc1489d8cfb1f119d34f1752022-12-22T02:48:25ZengElsevierEuropean Journal of Cell Biology0171-93352022-06-011013151235Microscopic characterization reveals the diversity of EVs secreted by GFP-HAS3 expressing MCF7 cellsJanne Capra0Kai Härkönen1Heikki Kyykallio2Helena Vihinen3Eija Jokitalo4Kirsi Rilla5Institute of Biomedicine, University of Eastern Finland, Kuopio, FinlandInstitute of Biomedicine, University of Eastern Finland, Kuopio, FinlandInstitute of Biomedicine, University of Eastern Finland, Kuopio, FinlandElectron Microscopy Unit, Institute of Biotechnology, University of Helsinki, Helsinki, FinlandElectron Microscopy Unit, Institute of Biotechnology, University of Helsinki, Helsinki, FinlandInstitute of Biomedicine, University of Eastern Finland, Kuopio, Finland; Correspondence to: Institute of Biomedicine, University of Eastern Finland, P.O.B. 1627, FIN-70211 Kuopio, Finland.We have shown the connection of hyaluronan synthesis activity with the enhanced shedding of extracellular vesicles, but detailed morphological analysis of those hyaluronan-induced EVs is still missing. In this study we utilized a comprehensive set of high-resolution imaging techniques to characterize in high detail the size and morphology of EVs originating from stable MCF7 breast cancer cell line and transiently transfected cells expressing GFP-HAS3. To avoid possible artefacts or loss of EVs resulting from the isolation process, special attention was paid to analysis of EVs in situ in monolayer and in 3D cultures. The results of this study show that GFP-HAS3 expressing MCF7 cells produce morphologically diverse EVs but also demonstrates the variation in results obtained with different experimental setup, which emphasizes the importance of comparison between different methods when interpreting the observations.http://www.sciencedirect.com/science/article/pii/S0171933522000383Extracellular vesicleMorphologyHyaluronan synthaseConfocal microscopy3D cultureElectron tomography |
| spellingShingle | Janne Capra Kai Härkönen Heikki Kyykallio Helena Vihinen Eija Jokitalo Kirsi Rilla Microscopic characterization reveals the diversity of EVs secreted by GFP-HAS3 expressing MCF7 cells European Journal of Cell Biology Extracellular vesicle Morphology Hyaluronan synthase Confocal microscopy 3D culture Electron tomography |
| title | Microscopic characterization reveals the diversity of EVs secreted by GFP-HAS3 expressing MCF7 cells |
| title_full | Microscopic characterization reveals the diversity of EVs secreted by GFP-HAS3 expressing MCF7 cells |
| title_fullStr | Microscopic characterization reveals the diversity of EVs secreted by GFP-HAS3 expressing MCF7 cells |
| title_full_unstemmed | Microscopic characterization reveals the diversity of EVs secreted by GFP-HAS3 expressing MCF7 cells |
| title_short | Microscopic characterization reveals the diversity of EVs secreted by GFP-HAS3 expressing MCF7 cells |
| title_sort | microscopic characterization reveals the diversity of evs secreted by gfp has3 expressing mcf7 cells |
| topic | Extracellular vesicle Morphology Hyaluronan synthase Confocal microscopy 3D culture Electron tomography |
| url | http://www.sciencedirect.com/science/article/pii/S0171933522000383 |
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