Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell
An innovative multi-step phase separation process was used to prepare tissue culture for the polystyrene-based, hierarchically structured substrates, which mimicked in vivo microenvironment and architecture. Macro- (pore area from 3000 to 18,000 µm<sup>2</sup>; roughness (Ra) 7.2 ± 0.1 µ...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/21/11943 |
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| author | Kateřina Skopalová Katarzyna Anna Radaszkiewicz Markéta Kadlečková Jiří Pacherník Antonín Minařík Zdenka Capáková Věra Kašpárková Aleš Mráček Eliška Daďová Petr Humpolíček |
| author_facet | Kateřina Skopalová Katarzyna Anna Radaszkiewicz Markéta Kadlečková Jiří Pacherník Antonín Minařík Zdenka Capáková Věra Kašpárková Aleš Mráček Eliška Daďová Petr Humpolíček |
| author_sort | Kateřina Skopalová |
| collection | DOAJ |
| description | An innovative multi-step phase separation process was used to prepare tissue culture for the polystyrene-based, hierarchically structured substrates, which mimicked in vivo microenvironment and architecture. Macro- (pore area from 3000 to 18,000 µm<sup>2</sup>; roughness (Ra) 7.2 ± 0.1 µm) and meso- (pore area from 50 to 300 µm<sup>2</sup>; Ra 1.1 ± 0.1 µm) structured substrates covered with micro-pores (area around 3 µm<sup>2</sup>) were prepared and characterised. Both types of substrate were suitable for human-induced pluripotent stem cell (hiPSC) cultivation and were found to be beneficial for the induction of cardiomyogenesis in hiPSC. This was confirmed both by the number of promoted proliferated cells and the expressions of specific markers (Nkx2.5, MYH6, MYL2, and MYL7). Moreover, the substrates amplified the fluorescence signal when Ca<sup>2+</sup> flow was monitored. This property, together with cytocompatibility, make this material especially suitable for in vitro studies of cell/material interactions within tissue-mimicking environments. |
| first_indexed | 2024-03-10T06:00:40Z |
| format | Article |
| id | doaj.art-63c113fdf8c44fd3898603fa17024988 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:00:40Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-63c113fdf8c44fd3898603fa170249882023-11-22T21:00:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122211194310.3390/ijms222111943Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem CellKateřina Skopalová0Katarzyna Anna Radaszkiewicz1Markéta Kadlečková2Jiří Pacherník3Antonín Minařík4Zdenka Capáková5Věra Kašpárková6Aleš Mráček7Eliška Daďová8Petr Humpolíček9Centre of Polymer Systems, Tomas Bata University in Zlin, 760 01 Zlin, Czech RepublicFaculty of Science, Masaryk University, 625 00 Brno, Czech RepublicCentre of Polymer Systems, Tomas Bata University in Zlin, 760 01 Zlin, Czech RepublicFaculty of Science, Masaryk University, 625 00 Brno, Czech RepublicCentre of Polymer Systems, Tomas Bata University in Zlin, 760 01 Zlin, Czech RepublicCentre of Polymer Systems, Tomas Bata University in Zlin, 760 01 Zlin, Czech RepublicCentre of Polymer Systems, Tomas Bata University in Zlin, 760 01 Zlin, Czech RepublicCentre of Polymer Systems, Tomas Bata University in Zlin, 760 01 Zlin, Czech RepublicCentre of Polymer Systems, Tomas Bata University in Zlin, 760 01 Zlin, Czech RepublicCentre of Polymer Systems, Tomas Bata University in Zlin, 760 01 Zlin, Czech RepublicAn innovative multi-step phase separation process was used to prepare tissue culture for the polystyrene-based, hierarchically structured substrates, which mimicked in vivo microenvironment and architecture. Macro- (pore area from 3000 to 18,000 µm<sup>2</sup>; roughness (Ra) 7.2 ± 0.1 µm) and meso- (pore area from 50 to 300 µm<sup>2</sup>; Ra 1.1 ± 0.1 µm) structured substrates covered with micro-pores (area around 3 µm<sup>2</sup>) were prepared and characterised. Both types of substrate were suitable for human-induced pluripotent stem cell (hiPSC) cultivation and were found to be beneficial for the induction of cardiomyogenesis in hiPSC. This was confirmed both by the number of promoted proliferated cells and the expressions of specific markers (Nkx2.5, MYH6, MYL2, and MYL7). Moreover, the substrates amplified the fluorescence signal when Ca<sup>2+</sup> flow was monitored. This property, together with cytocompatibility, make this material especially suitable for in vitro studies of cell/material interactions within tissue-mimicking environments.https://www.mdpi.com/1422-0067/22/21/11943biomimeticsurfaceshuman-induced pluripotent stem cellsfluorescence signalcardiomyogenesis |
| spellingShingle | Kateřina Skopalová Katarzyna Anna Radaszkiewicz Markéta Kadlečková Jiří Pacherník Antonín Minařík Zdenka Capáková Věra Kašpárková Aleš Mráček Eliška Daďová Petr Humpolíček Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell International Journal of Molecular Sciences biomimetic surfaces human-induced pluripotent stem cells fluorescence signal cardiomyogenesis |
| title | Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell |
| title_full | Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell |
| title_fullStr | Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell |
| title_full_unstemmed | Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell |
| title_short | Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell |
| title_sort | hierarchically structured polystyrene based surfaces amplifying fluorescence signals cytocompatibility with human induced pluripotent stem cell |
| topic | biomimetic surfaces human-induced pluripotent stem cells fluorescence signal cardiomyogenesis |
| url | https://www.mdpi.com/1422-0067/22/21/11943 |
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