In vitro prospective healthy and nutritional benefits of different Citrus monofloral honeys

Abstract We studied the total polyphenols, flavonoids, vitamin C, the antioxidant and anti-inflammatory activity of six Citrus monofloral honey, and the in vitro inhibitory effect against cholinesterases and tyrosinase. Finally, we assessed their effect against the biofilm of some pathogenic bacteri...

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Main Authors: Florinda Fratianni, Giuseppe Amato, Antonio d’Acierno, Maria Neve Ombra, Vincenzo De Feo, Raffaele Coppola, Filomena Nazzaro
Format: Article
Language:English
Published: Nature Portfolio 2023-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-27802-1
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author Florinda Fratianni
Giuseppe Amato
Antonio d’Acierno
Maria Neve Ombra
Vincenzo De Feo
Raffaele Coppola
Filomena Nazzaro
author_facet Florinda Fratianni
Giuseppe Amato
Antonio d’Acierno
Maria Neve Ombra
Vincenzo De Feo
Raffaele Coppola
Filomena Nazzaro
author_sort Florinda Fratianni
collection DOAJ
description Abstract We studied the total polyphenols, flavonoids, vitamin C, the antioxidant and anti-inflammatory activity of six Citrus monofloral honey, and the in vitro inhibitory effect against cholinesterases and tyrosinase. Finally, we assessed their effect against the biofilm of some pathogenic bacteria. Lime honey showed the best antioxidant activity and the highest content of polyphenols and vitamin C. Lemon and tangerine honey contained almost exclusively flavonoids. Lemon honey better preserved the bovine serum albumin against denaturation (IC50 = 48.47 mg). Honeys inhibited acetylcholinesterase, butyrylcholinesterase, and tyrosinase up to 12.04% (tangerine), 19.11% (bergamot), and 94.1% (lemon), respectively. Lime and clementine honey better inhibited the Listeria monocytogenes biofilm. Bergamot honey acted mainly against the Staphylococcus aureus and Acinetobacter baumannii biofilm; bergamot and tangerine honey inhibited the Pseudomonas aeruginosa biofilm particularly. Bergamot, clementine, and tangerine honey acted against Escherichia coli sessile cell metabolism. This Citrus honey exhibited in vitro prospective health benefits and is applicable for future in vivo studies.
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spelling doaj.art-63c1443947f24ae0a75c6b53b1ab6c902023-01-22T12:11:34ZengNature PortfolioScientific Reports2045-23222023-01-0113111010.1038/s41598-023-27802-1In vitro prospective healthy and nutritional benefits of different Citrus monofloral honeysFlorinda Fratianni0Giuseppe Amato1Antonio d’Acierno2Maria Neve Ombra3Vincenzo De Feo4Raffaele Coppola5Filomena Nazzaro6Institute of Food Sciences, CNR-ISAInstitute of Food Sciences, CNR-ISAInstitute of Food Sciences, CNR-ISAInstitute of Food Sciences, CNR-ISAInstitute of Food Sciences, CNR-ISAInstitute of Food Sciences, CNR-ISAInstitute of Food Sciences, CNR-ISAAbstract We studied the total polyphenols, flavonoids, vitamin C, the antioxidant and anti-inflammatory activity of six Citrus monofloral honey, and the in vitro inhibitory effect against cholinesterases and tyrosinase. Finally, we assessed their effect against the biofilm of some pathogenic bacteria. Lime honey showed the best antioxidant activity and the highest content of polyphenols and vitamin C. Lemon and tangerine honey contained almost exclusively flavonoids. Lemon honey better preserved the bovine serum albumin against denaturation (IC50 = 48.47 mg). Honeys inhibited acetylcholinesterase, butyrylcholinesterase, and tyrosinase up to 12.04% (tangerine), 19.11% (bergamot), and 94.1% (lemon), respectively. Lime and clementine honey better inhibited the Listeria monocytogenes biofilm. Bergamot honey acted mainly against the Staphylococcus aureus and Acinetobacter baumannii biofilm; bergamot and tangerine honey inhibited the Pseudomonas aeruginosa biofilm particularly. Bergamot, clementine, and tangerine honey acted against Escherichia coli sessile cell metabolism. This Citrus honey exhibited in vitro prospective health benefits and is applicable for future in vivo studies.https://doi.org/10.1038/s41598-023-27802-1
spellingShingle Florinda Fratianni
Giuseppe Amato
Antonio d’Acierno
Maria Neve Ombra
Vincenzo De Feo
Raffaele Coppola
Filomena Nazzaro
In vitro prospective healthy and nutritional benefits of different Citrus monofloral honeys
Scientific Reports
title In vitro prospective healthy and nutritional benefits of different Citrus monofloral honeys
title_full In vitro prospective healthy and nutritional benefits of different Citrus monofloral honeys
title_fullStr In vitro prospective healthy and nutritional benefits of different Citrus monofloral honeys
title_full_unstemmed In vitro prospective healthy and nutritional benefits of different Citrus monofloral honeys
title_short In vitro prospective healthy and nutritional benefits of different Citrus monofloral honeys
title_sort in vitro prospective healthy and nutritional benefits of different citrus monofloral honeys
url https://doi.org/10.1038/s41598-023-27802-1
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