Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo
Rewiring neural circuits by the formation and elimination of synapses is thought to be a key cellular mechanism of learning and memory in the mammalian brain. Dendritic spines are the postsynaptic structural component of excitatory synapses, and their experience-dependent plasticity has been extensi...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-06-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/34700 |
| _version_ | 1811236879924723712 |
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| author | Thomas Pfeiffer Stefanie Poll Stephane Bancelin Julie Angibaud VVG Krishna Inavalli Kevin Keppler Manuel Mittag Martin Fuhrmann U Valentin Nägerl |
| author_facet | Thomas Pfeiffer Stefanie Poll Stephane Bancelin Julie Angibaud VVG Krishna Inavalli Kevin Keppler Manuel Mittag Martin Fuhrmann U Valentin Nägerl |
| author_sort | Thomas Pfeiffer |
| collection | DOAJ |
| description | Rewiring neural circuits by the formation and elimination of synapses is thought to be a key cellular mechanism of learning and memory in the mammalian brain. Dendritic spines are the postsynaptic structural component of excitatory synapses, and their experience-dependent plasticity has been extensively studied in mouse superficial cortex using two-photon microscopy in vivo. By contrast, very little is known about spine plasticity in the hippocampus, which is the archetypical memory center of the brain, mostly because it is difficult to visualize dendritic spines in this deeply embedded structure with sufficient spatial resolution. We developed chronic 2P-STED microscopy in mouse hippocampus, using a ‘hippocampal window’ based on resection of cortical tissue and a long working distance objective for optical access. We observed a two-fold higher spine density than previous studies and measured a spine turnover of ~40% within 4 days, which depended on spine size. We thus provide direct evidence for a high level of structural rewiring of synaptic circuits and new insights into the structure-dynamics relationship of hippocampal spines. Having established chronic super-resolution microscopy in the hippocampus in vivo, our study enables longitudinal and correlative analyses of nanoscale neuroanatomical structures with genetic, molecular and behavioral experiments. |
| first_indexed | 2024-04-12T12:15:44Z |
| format | Article |
| id | doaj.art-63c2386c7d1245d9af3b2f5e1f6a4d22 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:15:44Z |
| publishDate | 2018-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-63c2386c7d1245d9af3b2f5e1f6a4d222022-12-22T03:33:26ZengeLife Sciences Publications LtdeLife2050-084X2018-06-01710.7554/eLife.34700Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivoThomas Pfeiffer0https://orcid.org/0000-0002-0571-4139Stefanie Poll1https://orcid.org/0000-0001-5301-2791Stephane Bancelin2https://orcid.org/0000-0001-6328-0423Julie Angibaud3VVG Krishna Inavalli4Kevin Keppler5Manuel Mittag6Martin Fuhrmann7https://orcid.org/0000-0001-7672-2913U Valentin Nägerl8https://orcid.org/0000-0001-6831-9008Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, Bordeaux, France; Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, FranceNeuroimmunology and Imaging Group, German Center for Neurodegenerative Diseases, Bonn, GermanyInterdisciplinary Institute for Neuroscience, CNRS UMR 5297, Bordeaux, France; Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, FranceInterdisciplinary Institute for Neuroscience, CNRS UMR 5297, Bordeaux, France; Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, FranceInterdisciplinary Institute for Neuroscience, CNRS UMR 5297, Bordeaux, France; Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, FranceLight Microscope Facility, German Center for Neurodegenerative Diseases, Bonn, GermanyNeuroimmunology and Imaging Group, German Center for Neurodegenerative Diseases, Bonn, GermanyNeuroimmunology and Imaging Group, German Center for Neurodegenerative Diseases, Bonn, GermanyInterdisciplinary Institute for Neuroscience, CNRS UMR 5297, Bordeaux, France; Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, FranceRewiring neural circuits by the formation and elimination of synapses is thought to be a key cellular mechanism of learning and memory in the mammalian brain. Dendritic spines are the postsynaptic structural component of excitatory synapses, and their experience-dependent plasticity has been extensively studied in mouse superficial cortex using two-photon microscopy in vivo. By contrast, very little is known about spine plasticity in the hippocampus, which is the archetypical memory center of the brain, mostly because it is difficult to visualize dendritic spines in this deeply embedded structure with sufficient spatial resolution. We developed chronic 2P-STED microscopy in mouse hippocampus, using a ‘hippocampal window’ based on resection of cortical tissue and a long working distance objective for optical access. We observed a two-fold higher spine density than previous studies and measured a spine turnover of ~40% within 4 days, which depended on spine size. We thus provide direct evidence for a high level of structural rewiring of synaptic circuits and new insights into the structure-dynamics relationship of hippocampal spines. Having established chronic super-resolution microscopy in the hippocampus in vivo, our study enables longitudinal and correlative analyses of nanoscale neuroanatomical structures with genetic, molecular and behavioral experiments.https://elifesciences.org/articles/34700hippocampussuper-resolution microscopyspine plasticityin vivo imagingsynapseslearning and memory |
| spellingShingle | Thomas Pfeiffer Stefanie Poll Stephane Bancelin Julie Angibaud VVG Krishna Inavalli Kevin Keppler Manuel Mittag Martin Fuhrmann U Valentin Nägerl Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo eLife hippocampus super-resolution microscopy spine plasticity in vivo imaging synapses learning and memory |
| title | Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo |
| title_full | Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo |
| title_fullStr | Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo |
| title_full_unstemmed | Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo |
| title_short | Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo |
| title_sort | chronic 2p sted imaging reveals high turnover of dendritic spines in the hippocampus in vivo |
| topic | hippocampus super-resolution microscopy spine plasticity in vivo imaging synapses learning and memory |
| url | https://elifesciences.org/articles/34700 |
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