Gibberellins Promote Brassinosteroids Action and Both Increase Heterosis for Plant Height in Maize (Zea mays L.)
Brassinosteroids (BRs) and Gibberellins (GAs) are two classes of plant hormones affecting plant height (PHT). Thus, manipulation of BR and GA levels or signaling enables optimization of crop grain and biomass yields. We established backcross (BC) families, selected for increased PHT, in two elite ma...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01039/full |
| _version_ | 1818547542131998720 |
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| author | Songlin Hu Cuiling Wang Darlene L. Sanchez Alexander E. Lipka Peng Liu Yanhai Yin Michael Blanco Thomas Lübberstedt |
| author_facet | Songlin Hu Cuiling Wang Darlene L. Sanchez Alexander E. Lipka Peng Liu Yanhai Yin Michael Blanco Thomas Lübberstedt |
| author_sort | Songlin Hu |
| collection | DOAJ |
| description | Brassinosteroids (BRs) and Gibberellins (GAs) are two classes of plant hormones affecting plant height (PHT). Thus, manipulation of BR and GA levels or signaling enables optimization of crop grain and biomass yields. We established backcross (BC) families, selected for increased PHT, in two elite maize inbred backgrounds. Various exotic accessions used in the germplasm enhancement in maize project served as donors. BC1-derived doubled haploid lines in the same two elite maize inbred backgrounds established without selection for plant height were included for comparison. We conducted genome-wide association studies to explore the genetic control of PHT by BR and GA. In addition, we used BR and GA inhibitors to compare the relationship between PHT, BR, and GA in inbred lines and heterozygotes from a physiological and biological perspective. A total of 73 genomic loci were discovered to be associated with PHT, with seven co-localized with GA, and two co-localized with BR candidate genes. PHT determined in field trials was significantly correlated with seedling stage BR and GA inhibitor responses. However, this observation was only true for maize heterozygotes, not for inbred lines. Path analysis results suggest that heterozygosity increases GA levels, which in turn promote BR levels. Thus, at least part of heterosis for PHT in maize can be explained by increased GA and BR levels, and seedling stage hormone inhibitor response is promising to predict heterosis for PHT. |
| first_indexed | 2024-12-12T08:08:08Z |
| format | Article |
| id | doaj.art-63c32e17fb25445196c1a238bee41f59 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T08:08:08Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-63c32e17fb25445196c1a238bee41f592022-12-22T00:31:53ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-06-01810.3389/fpls.2017.01039271452Gibberellins Promote Brassinosteroids Action and Both Increase Heterosis for Plant Height in Maize (Zea mays L.)Songlin Hu0Cuiling Wang1Darlene L. Sanchez2Alexander E. Lipka3Peng Liu4Yanhai Yin5Michael Blanco6Thomas Lübberstedt7Department of Agronomy, Iowa State University, AmesIA, United StatesDepartment of Agronomy, Henan University of Science and TechnologyLuoyang, ChinaDepartment of Agronomy, Iowa State University, AmesIA, United StatesDepartment of Crop Sciences, University of Illinois at Urbana–Champaign, ChampaignIL, United StatesDepartment of Statistics, Iowa State University, AmesIA, United StatesDepartment of Genetics, Development and Cell biology, Iowa State University, AmesIA, United StatesPlant Introduction Research Unit, Department of Agronomy, United States Department of Agriculture – Agricultural Research Service, Iowa State University, AmesIA, United StatesDepartment of Agronomy, Iowa State University, AmesIA, United StatesBrassinosteroids (BRs) and Gibberellins (GAs) are two classes of plant hormones affecting plant height (PHT). Thus, manipulation of BR and GA levels or signaling enables optimization of crop grain and biomass yields. We established backcross (BC) families, selected for increased PHT, in two elite maize inbred backgrounds. Various exotic accessions used in the germplasm enhancement in maize project served as donors. BC1-derived doubled haploid lines in the same two elite maize inbred backgrounds established without selection for plant height were included for comparison. We conducted genome-wide association studies to explore the genetic control of PHT by BR and GA. In addition, we used BR and GA inhibitors to compare the relationship between PHT, BR, and GA in inbred lines and heterozygotes from a physiological and biological perspective. A total of 73 genomic loci were discovered to be associated with PHT, with seven co-localized with GA, and two co-localized with BR candidate genes. PHT determined in field trials was significantly correlated with seedling stage BR and GA inhibitor responses. However, this observation was only true for maize heterozygotes, not for inbred lines. Path analysis results suggest that heterozygosity increases GA levels, which in turn promote BR levels. Thus, at least part of heterosis for PHT in maize can be explained by increased GA and BR levels, and seedling stage hormone inhibitor response is promising to predict heterosis for PHT.http://journal.frontiersin.org/article/10.3389/fpls.2017.01039/fullbrassinosteroidgibberellinplant heightgenome-wide association studyheterosis |
| spellingShingle | Songlin Hu Cuiling Wang Darlene L. Sanchez Alexander E. Lipka Peng Liu Yanhai Yin Michael Blanco Thomas Lübberstedt Gibberellins Promote Brassinosteroids Action and Both Increase Heterosis for Plant Height in Maize (Zea mays L.) Frontiers in Plant Science brassinosteroid gibberellin plant height genome-wide association study heterosis |
| title | Gibberellins Promote Brassinosteroids Action and Both Increase Heterosis for Plant Height in Maize (Zea mays L.) |
| title_full | Gibberellins Promote Brassinosteroids Action and Both Increase Heterosis for Plant Height in Maize (Zea mays L.) |
| title_fullStr | Gibberellins Promote Brassinosteroids Action and Both Increase Heterosis for Plant Height in Maize (Zea mays L.) |
| title_full_unstemmed | Gibberellins Promote Brassinosteroids Action and Both Increase Heterosis for Plant Height in Maize (Zea mays L.) |
| title_short | Gibberellins Promote Brassinosteroids Action and Both Increase Heterosis for Plant Height in Maize (Zea mays L.) |
| title_sort | gibberellins promote brassinosteroids action and both increase heterosis for plant height in maize zea mays l |
| topic | brassinosteroid gibberellin plant height genome-wide association study heterosis |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01039/full |
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