Cell-free miRNAs may indicate diagnosis and docetaxel sensitivity of tumor cells in malignant effusions
<p>Abstract</p> <p>Background</p> <p>Circulating cell-free microRNAs have been identified as potential cancer biomarkers. However, the existence and the potential application of cell-free miRNAs in effusion samples are still uncertain. In order to explore the potential...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-10-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/10/591 |
| _version_ | 1818485707968086016 |
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| author | Ding Yitao Yu Lixia Wei Jia Wang Tingting Qian Xiaoping Wang Lifeng Chen Xi Xie Li Zhang Chenyu Liu Baorui |
| author_facet | Ding Yitao Yu Lixia Wei Jia Wang Tingting Qian Xiaoping Wang Lifeng Chen Xi Xie Li Zhang Chenyu Liu Baorui |
| author_sort | Ding Yitao |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Circulating cell-free microRNAs have been identified as potential cancer biomarkers. However, the existence and the potential application of cell-free miRNAs in effusion samples are still uncertain. In order to explore the potential role of cell-free miRNA in malignant effusions, we selected 22 miRNAs differentially expressed in the serum of lung cancer patients and studied their expression levels in body cavity effusion samples.</p> <p>Methods</p> <p>We measured the expression of 22 miRNAs using qRT-PCR in two samples, which were pooled with 18 malignant and 12 benign effusions, respectively. After discarding 9 lowly expressed miRNAs, a panel of 13 miRNAs were measured in 29 samples (benign n = 11, malignant n = 18). We also carried out a WST-8 test to evaluate the docetaxel sensitivity of tumor cells directly isolated from 15 malignant effusions.</p> <p>Results</p> <p>We compared the miRNA expression levels between benign and malignant effusions using a Mann-Whitney U test and found miR-24, miR-26a and miR-30d were expressed differently between the two groups (<it>P </it>= 0.006, 0.021 and 0.011, respectively). Cells isolated from effusions rich in cell-free miR-152 were more sensitive to docetaxel (r = 0.60, <it>P </it>= 0.016).</p> <p>Conclusions</p> <p>Collectively, our study demonstrated that cell-free miRNAs in the supernatant of effusions may aid in the diagnosis of malignancy and predict chemosensitivity to docetaxel.</p> |
| first_indexed | 2024-12-10T16:12:32Z |
| format | Article |
| id | doaj.art-63c33466dcc84a03adace126d4853b5c |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-10T16:12:32Z |
| publishDate | 2010-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-63c33466dcc84a03adace126d4853b5c2022-12-22T01:42:04ZengBMCBMC Cancer1471-24072010-10-0110159110.1186/1471-2407-10-591Cell-free miRNAs may indicate diagnosis and docetaxel sensitivity of tumor cells in malignant effusionsDing YitaoYu LixiaWei JiaWang TingtingQian XiaopingWang LifengChen XiXie LiZhang ChenyuLiu Baorui<p>Abstract</p> <p>Background</p> <p>Circulating cell-free microRNAs have been identified as potential cancer biomarkers. However, the existence and the potential application of cell-free miRNAs in effusion samples are still uncertain. In order to explore the potential role of cell-free miRNA in malignant effusions, we selected 22 miRNAs differentially expressed in the serum of lung cancer patients and studied their expression levels in body cavity effusion samples.</p> <p>Methods</p> <p>We measured the expression of 22 miRNAs using qRT-PCR in two samples, which were pooled with 18 malignant and 12 benign effusions, respectively. After discarding 9 lowly expressed miRNAs, a panel of 13 miRNAs were measured in 29 samples (benign n = 11, malignant n = 18). We also carried out a WST-8 test to evaluate the docetaxel sensitivity of tumor cells directly isolated from 15 malignant effusions.</p> <p>Results</p> <p>We compared the miRNA expression levels between benign and malignant effusions using a Mann-Whitney U test and found miR-24, miR-26a and miR-30d were expressed differently between the two groups (<it>P </it>= 0.006, 0.021 and 0.011, respectively). Cells isolated from effusions rich in cell-free miR-152 were more sensitive to docetaxel (r = 0.60, <it>P </it>= 0.016).</p> <p>Conclusions</p> <p>Collectively, our study demonstrated that cell-free miRNAs in the supernatant of effusions may aid in the diagnosis of malignancy and predict chemosensitivity to docetaxel.</p>http://www.biomedcentral.com/1471-2407/10/591 |
| spellingShingle | Ding Yitao Yu Lixia Wei Jia Wang Tingting Qian Xiaoping Wang Lifeng Chen Xi Xie Li Zhang Chenyu Liu Baorui Cell-free miRNAs may indicate diagnosis and docetaxel sensitivity of tumor cells in malignant effusions BMC Cancer |
| title | Cell-free miRNAs may indicate diagnosis and docetaxel sensitivity of tumor cells in malignant effusions |
| title_full | Cell-free miRNAs may indicate diagnosis and docetaxel sensitivity of tumor cells in malignant effusions |
| title_fullStr | Cell-free miRNAs may indicate diagnosis and docetaxel sensitivity of tumor cells in malignant effusions |
| title_full_unstemmed | Cell-free miRNAs may indicate diagnosis and docetaxel sensitivity of tumor cells in malignant effusions |
| title_short | Cell-free miRNAs may indicate diagnosis and docetaxel sensitivity of tumor cells in malignant effusions |
| title_sort | cell free mirnas may indicate diagnosis and docetaxel sensitivity of tumor cells in malignant effusions |
| url | http://www.biomedcentral.com/1471-2407/10/591 |
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