Side population rather than CD133<sup>+ </sup>cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells
<p>Abstract</p> <p>Background</p> <p>Subpopulations of cancer cells with the capacity of generating solid tumors have been characterized. In various cancer types, including prostate cancer cells, a side population (SP) and CD133-expressing cells have been proposed as co...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-09-01
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| Series: | Molecular Cancer |
| Subjects: | |
| Online Access: | http://www.molecular-cancer.com/content/10/1/112 |
| _version_ | 1811249673437970432 |
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| author | Wolcott Karen Cannon Virginetta Wang Honghe Zhou Jianjun Song Hongbin Yates Clayton |
| author_facet | Wolcott Karen Cannon Virginetta Wang Honghe Zhou Jianjun Song Hongbin Yates Clayton |
| author_sort | Wolcott Karen |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Subpopulations of cancer cells with the capacity of generating solid tumors have been characterized. In various cancer types, including prostate cancer cells, a side population (SP) and CD133-expressing cells have been proposed as containing a population cancer cells with stem-like ability. Therefore the aim of this work was to determine, in prostate cancer cell lines, the frequency and tumorigenic potential of SP and CD133+ cells.</p> <p>Results</p> <p><it>In vitro </it>2D colony-forming assay and sphere-forming assay, Flow cytometry analysis and magnetic cell sorting were utilized to sort CD133+, CD133- and Side population (SP) cells. Our findings indicate that CD44 and integrin α-6 are uniformly expressed in the hTERT cell lines; however, CD133 is expressed only in a small population (< 0.1%). FACS-sorted CD133+ and CD133- cells exhibited similar tumorigenicity <it>in vitro </it>and <it>in vivo</it>. Additionally, for the hTERT cells, SP rather than CD133 expression showed an 8-fold enhanced tumorigenic potential. The data suggest that SP cells, rather than those with CD133 marker, contain the rare population of CSC capable of producing prostate tumors.</p> <p>Conclusion</p> <p>Collectively, our data suggest that although CD133 is expressed only in a small population of hTERT-immortalized prostate cancer cells, it is not likely to be associated with stem cells, as CD133- and CD133+ cells exhibited similar tumorigenicity. However, SP isolated cells, appear to be enriched with tumorigenic stem-like cells capable of generating palpable tumors.</p> |
| first_indexed | 2024-04-12T15:50:43Z |
| format | Article |
| id | doaj.art-63c3bb637a924145b0bc4d2c0bee5f20 |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-04-12T15:50:43Z |
| publishDate | 2011-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-63c3bb637a924145b0bc4d2c0bee5f202022-12-22T03:26:30ZengBMCMolecular Cancer1476-45982011-09-0110111210.1186/1476-4598-10-112Side population rather than CD133<sup>+ </sup>cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cellsWolcott KarenCannon VirginettaWang HongheZhou JianjunSong HongbinYates Clayton<p>Abstract</p> <p>Background</p> <p>Subpopulations of cancer cells with the capacity of generating solid tumors have been characterized. In various cancer types, including prostate cancer cells, a side population (SP) and CD133-expressing cells have been proposed as containing a population cancer cells with stem-like ability. Therefore the aim of this work was to determine, in prostate cancer cell lines, the frequency and tumorigenic potential of SP and CD133+ cells.</p> <p>Results</p> <p><it>In vitro </it>2D colony-forming assay and sphere-forming assay, Flow cytometry analysis and magnetic cell sorting were utilized to sort CD133+, CD133- and Side population (SP) cells. Our findings indicate that CD44 and integrin α-6 are uniformly expressed in the hTERT cell lines; however, CD133 is expressed only in a small population (< 0.1%). FACS-sorted CD133+ and CD133- cells exhibited similar tumorigenicity <it>in vitro </it>and <it>in vivo</it>. Additionally, for the hTERT cells, SP rather than CD133 expression showed an 8-fold enhanced tumorigenic potential. The data suggest that SP cells, rather than those with CD133 marker, contain the rare population of CSC capable of producing prostate tumors.</p> <p>Conclusion</p> <p>Collectively, our data suggest that although CD133 is expressed only in a small population of hTERT-immortalized prostate cancer cells, it is not likely to be associated with stem cells, as CD133- and CD133+ cells exhibited similar tumorigenicity. However, SP isolated cells, appear to be enriched with tumorigenic stem-like cells capable of generating palpable tumors.</p>http://www.molecular-cancer.com/content/10/1/112Cancer Stem CellsCD133Side population (SP)prostate cancer |
| spellingShingle | Wolcott Karen Cannon Virginetta Wang Honghe Zhou Jianjun Song Hongbin Yates Clayton Side population rather than CD133<sup>+ </sup>cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells Molecular Cancer Cancer Stem Cells CD133 Side population (SP) prostate cancer |
| title | Side population rather than CD133<sup>+ </sup>cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells |
| title_full | Side population rather than CD133<sup>+ </sup>cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells |
| title_fullStr | Side population rather than CD133<sup>+ </sup>cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells |
| title_full_unstemmed | Side population rather than CD133<sup>+ </sup>cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells |
| title_short | Side population rather than CD133<sup>+ </sup>cells distinguishes enriched tumorigenicity in hTERT-immortalized primary prostate cancer cells |
| title_sort | side population rather than cd133 sup sup cells distinguishes enriched tumorigenicity in htert immortalized primary prostate cancer cells |
| topic | Cancer Stem Cells CD133 Side population (SP) prostate cancer |
| url | http://www.molecular-cancer.com/content/10/1/112 |
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