Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L.
Jatropha curcas L. belongs to Euphorbiaceae family, and it synthesizes flavonoid and diterpene compounds that have showed antioxidant, anti-inflammatory, anticancer, antiviral, antimicrobial, antifungal and insecticide activity. Seeds of this plant accumulate phorbol esters, which are tigliane type...
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PeerJ Inc.
2020-11-01
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| Online Access: | https://peerj.com/articles/10172.pdf |
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| author | Gerardo Leyva-Padrón Pablo Emilio Vanegas-Espinoza Silvia Evangelista-Lozano Alma Angélica Del Villar-Martínez Crescencio Bazaldúa |
| author_facet | Gerardo Leyva-Padrón Pablo Emilio Vanegas-Espinoza Silvia Evangelista-Lozano Alma Angélica Del Villar-Martínez Crescencio Bazaldúa |
| author_sort | Gerardo Leyva-Padrón |
| collection | DOAJ |
| description | Jatropha curcas L. belongs to Euphorbiaceae family, and it synthesizes flavonoid and diterpene compounds that have showed antioxidant, anti-inflammatory, anticancer, antiviral, antimicrobial, antifungal and insecticide activity. Seeds of this plant accumulate phorbol esters, which are tigliane type diterpenes, reported as toxic and, depending on its concentration, toxic and non-toxic varieties has been identified. The aim of this work was to characterize the chemical profile of the extracts from seeds, leaves and callus of both varieties (toxic and non-toxic) of Jatropha curcas, to verify the presence of important compounds in dedifferentiated cells and consider the possibility of using these cultures for the massive production of metabolites. Callus induction was obtained using NAA (1.5 mg L−1) and BAP (1.5 mg L−1) after 21 d for both varieties. Thin layer chromatography analysis showed differences in compounds accumulation in callus from non-toxic variety throughout the time of culture, diterpenes showed an increase along the time, in contrast with flavonoids which decreased. Based on the results obtained through microQTOF-QII spectrometer it is suggested a higher accumulation of phorbol esters, derived from 12-deoxy-16-hydroxy-phorbol (m/z 365 [M+H]+), in callus of 38 d than those of 14 d culture, from both varieties. Unlike flavonoids accumulation, the MS chromatograms analysis allowed to suggest lower accumulation of flavonoids as the culture time progresses, in callus from both varieties. The presence of six glycosylated flavonoids is also suggested in leaf and callus extracts derived from both varieties (toxic and non-toxic), including: apigenin 6-C-α-L-arabinopyranosyl-8-C-β-D-xylopyranoside (m/z 535 [M+H]+), apigenin 4′-O-rhamnoside (m/z 417 [M+H]+), vitexin (m/z 433 [M+H]+), vitexin 4′-O-glucoside-2″-O-rhamnoside (m/z 741 [M+H]+), vicenin-2 (m/z 595 [M+H]+), and vicenin-2,6″-O-glucoside (m/z 757 [M+H]+). |
| first_indexed | 2024-03-09T07:21:53Z |
| format | Article |
| id | doaj.art-63c3bbf6dd894549afbd59a7cb51268d |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:21:53Z |
| publishDate | 2020-11-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-63c3bbf6dd894549afbd59a7cb51268d2023-12-03T07:15:20ZengPeerJ Inc.PeerJ2167-83592020-11-018e1017210.7717/peerj.10172Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L.Gerardo Leyva-Padrón0Pablo Emilio Vanegas-Espinoza1Silvia Evangelista-Lozano2Alma Angélica Del Villar-Martínez3Crescencio Bazaldúa4Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Yautepec, Morelos, MéxicoCentro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Yautepec, Morelos, MéxicoCentro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Yautepec, Morelos, MéxicoCentro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Yautepec, Morelos, MéxicoCentro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Yautepec, Morelos, MéxicoJatropha curcas L. belongs to Euphorbiaceae family, and it synthesizes flavonoid and diterpene compounds that have showed antioxidant, anti-inflammatory, anticancer, antiviral, antimicrobial, antifungal and insecticide activity. Seeds of this plant accumulate phorbol esters, which are tigliane type diterpenes, reported as toxic and, depending on its concentration, toxic and non-toxic varieties has been identified. The aim of this work was to characterize the chemical profile of the extracts from seeds, leaves and callus of both varieties (toxic and non-toxic) of Jatropha curcas, to verify the presence of important compounds in dedifferentiated cells and consider the possibility of using these cultures for the massive production of metabolites. Callus induction was obtained using NAA (1.5 mg L−1) and BAP (1.5 mg L−1) after 21 d for both varieties. Thin layer chromatography analysis showed differences in compounds accumulation in callus from non-toxic variety throughout the time of culture, diterpenes showed an increase along the time, in contrast with flavonoids which decreased. Based on the results obtained through microQTOF-QII spectrometer it is suggested a higher accumulation of phorbol esters, derived from 12-deoxy-16-hydroxy-phorbol (m/z 365 [M+H]+), in callus of 38 d than those of 14 d culture, from both varieties. Unlike flavonoids accumulation, the MS chromatograms analysis allowed to suggest lower accumulation of flavonoids as the culture time progresses, in callus from both varieties. The presence of six glycosylated flavonoids is also suggested in leaf and callus extracts derived from both varieties (toxic and non-toxic), including: apigenin 6-C-α-L-arabinopyranosyl-8-C-β-D-xylopyranoside (m/z 535 [M+H]+), apigenin 4′-O-rhamnoside (m/z 417 [M+H]+), vitexin (m/z 433 [M+H]+), vitexin 4′-O-glucoside-2″-O-rhamnoside (m/z 741 [M+H]+), vicenin-2 (m/z 595 [M+H]+), and vicenin-2,6″-O-glucoside (m/z 757 [M+H]+).https://peerj.com/articles/10172.pdfJatropha curcasmicrOTOF Q-IICallusPhorbol estersGlycosilated flavonoids |
| spellingShingle | Gerardo Leyva-Padrón Pablo Emilio Vanegas-Espinoza Silvia Evangelista-Lozano Alma Angélica Del Villar-Martínez Crescencio Bazaldúa Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L. PeerJ Jatropha curcas micrOTOF Q-II Callus Phorbol esters Glycosilated flavonoids |
| title | Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L. |
| title_full | Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L. |
| title_fullStr | Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L. |
| title_full_unstemmed | Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L. |
| title_short | Chemical analysis of callus extracts from toxic and non-toxic varieties of Jatropha curcas L. |
| title_sort | chemical analysis of callus extracts from toxic and non toxic varieties of jatropha curcas l |
| topic | Jatropha curcas micrOTOF Q-II Callus Phorbol esters Glycosilated flavonoids |
| url | https://peerj.com/articles/10172.pdf |
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