Long Non-Coding RNA TMPO-AS1 Promotes Cell Migration and Invasion by Sponging miR-140-5p and Inducing SOX4-Mediated EMT in Gastric Cancer
Yonghong Sun, Chunyao Han Department of General Surgery, Second Hospital of Shanxi Medical University, Taiyuan City, Shanxi Province 030001, People’s Republic of ChinaCorrespondence: Yonghong SunDepartment of General Surgery, Second Hospital of Shanxi Medical University, No. 382 Wuyi Road,...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2020-02-01
|
| Series: | Cancer Management and Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/long-non-coding-rna-tmpo-as1-promotes-cell-migration-and-invasion-by-s-peer-reviewed-article-CMAR |
| _version_ | 1819210047969820672 |
|---|---|
| author | Sun Y Han C |
| author_facet | Sun Y Han C |
| author_sort | Sun Y |
| collection | DOAJ |
| description | Yonghong Sun, Chunyao Han Department of General Surgery, Second Hospital of Shanxi Medical University, Taiyuan City, Shanxi Province 030001, People’s Republic of ChinaCorrespondence: Yonghong SunDepartment of General Surgery, Second Hospital of Shanxi Medical University, No. 382 Wuyi Road, Taiyuan City 030001, Shanxi Province, People’s Republic of ChinaEmail sunyonghlaxsy@163.comBackground: Mounting evidence show that long non-coding RNAs (lncRNAs) play critical roles in the progression of various human cancers, including gastric cancer (GC), a common gastrointestinal tumor. In this study, the biological functions of lncRNA TMPO-AS1 in GC were studied.Methods: TMPO-AS1 and miR-140-5p expression levels were detected in GC tissues and cell lines by RT-qPCR analysis. Knockdown or overexpression of TMPO-AS1 was conducted to evaluate the effects of TMPO-AS1 on the malignant behaviors of GC cells. Bioinformatic prediction and dual-luciferase reporter assay were performed to investigate the direct interaction between TMPO-AS1 and miR-140-5p in GC.Results: We observed that TMPO-AS1 was up-regulated in GC tissues, and high TMPO-AS1 expression in GC patients was closely correlated with aggressive clinicopathologic characteristics and poor overall survival. Functionally, gain- and loss-of-function studies showed that TMPO-AS1 overexpression enhanced the proliferation, migration, invasion and EMT of GC cells in vitro, whereas knockdown of TMPO-AS1 inhibited these malignant traits. Importantly, we demonstrated that TMPO-AS1 could function as a competing endogenous RNA (ceRNA) by sponging miR-140-5p in GC cells, thereby diminishing the inhibition on SOX4, an EMT regulator.Conclusion: Our findings indicated that TMPO-AS1 promotes GC progression partly by regulating miR-140-5p/SOX4 axis, and may serve as a novel therapeutic target for GC.Keywords: gastric cancer, long non-coding RNA TMPO-AS1, miR-140-5p, SOX4, EMT |
| first_indexed | 2024-12-23T06:04:58Z |
| format | Article |
| id | doaj.art-63c87f1742ab4da8b0c661bd9d547a12 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-23T06:04:58Z |
| publishDate | 2020-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-63c87f1742ab4da8b0c661bd9d547a122022-12-21T17:57:35ZengDove Medical PressCancer Management and Research1179-13222020-02-01Volume 121261126851981Long Non-Coding RNA TMPO-AS1 Promotes Cell Migration and Invasion by Sponging miR-140-5p and Inducing SOX4-Mediated EMT in Gastric CancerSun YHan CYonghong Sun, Chunyao Han Department of General Surgery, Second Hospital of Shanxi Medical University, Taiyuan City, Shanxi Province 030001, People’s Republic of ChinaCorrespondence: Yonghong SunDepartment of General Surgery, Second Hospital of Shanxi Medical University, No. 382 Wuyi Road, Taiyuan City 030001, Shanxi Province, People’s Republic of ChinaEmail sunyonghlaxsy@163.comBackground: Mounting evidence show that long non-coding RNAs (lncRNAs) play critical roles in the progression of various human cancers, including gastric cancer (GC), a common gastrointestinal tumor. In this study, the biological functions of lncRNA TMPO-AS1 in GC were studied.Methods: TMPO-AS1 and miR-140-5p expression levels were detected in GC tissues and cell lines by RT-qPCR analysis. Knockdown or overexpression of TMPO-AS1 was conducted to evaluate the effects of TMPO-AS1 on the malignant behaviors of GC cells. Bioinformatic prediction and dual-luciferase reporter assay were performed to investigate the direct interaction between TMPO-AS1 and miR-140-5p in GC.Results: We observed that TMPO-AS1 was up-regulated in GC tissues, and high TMPO-AS1 expression in GC patients was closely correlated with aggressive clinicopathologic characteristics and poor overall survival. Functionally, gain- and loss-of-function studies showed that TMPO-AS1 overexpression enhanced the proliferation, migration, invasion and EMT of GC cells in vitro, whereas knockdown of TMPO-AS1 inhibited these malignant traits. Importantly, we demonstrated that TMPO-AS1 could function as a competing endogenous RNA (ceRNA) by sponging miR-140-5p in GC cells, thereby diminishing the inhibition on SOX4, an EMT regulator.Conclusion: Our findings indicated that TMPO-AS1 promotes GC progression partly by regulating miR-140-5p/SOX4 axis, and may serve as a novel therapeutic target for GC.Keywords: gastric cancer, long non-coding RNA TMPO-AS1, miR-140-5p, SOX4, EMThttps://www.dovepress.com/long-non-coding-rna-tmpo-as1-promotes-cell-migration-and-invasion-by-s-peer-reviewed-article-CMARgastric cancerlong non-coding rna tmpo-as1mir-140-5psox4emt |
| spellingShingle | Sun Y Han C Long Non-Coding RNA TMPO-AS1 Promotes Cell Migration and Invasion by Sponging miR-140-5p and Inducing SOX4-Mediated EMT in Gastric Cancer Cancer Management and Research gastric cancer long non-coding rna tmpo-as1 mir-140-5p sox4 emt |
| title | Long Non-Coding RNA TMPO-AS1 Promotes Cell Migration and Invasion by Sponging miR-140-5p and Inducing SOX4-Mediated EMT in Gastric Cancer |
| title_full | Long Non-Coding RNA TMPO-AS1 Promotes Cell Migration and Invasion by Sponging miR-140-5p and Inducing SOX4-Mediated EMT in Gastric Cancer |
| title_fullStr | Long Non-Coding RNA TMPO-AS1 Promotes Cell Migration and Invasion by Sponging miR-140-5p and Inducing SOX4-Mediated EMT in Gastric Cancer |
| title_full_unstemmed | Long Non-Coding RNA TMPO-AS1 Promotes Cell Migration and Invasion by Sponging miR-140-5p and Inducing SOX4-Mediated EMT in Gastric Cancer |
| title_short | Long Non-Coding RNA TMPO-AS1 Promotes Cell Migration and Invasion by Sponging miR-140-5p and Inducing SOX4-Mediated EMT in Gastric Cancer |
| title_sort | long non coding rna tmpo as1 promotes cell migration and invasion by sponging mir 140 5p and inducing sox4 mediated emt in gastric cancer |
| topic | gastric cancer long non-coding rna tmpo-as1 mir-140-5p sox4 emt |
| url | https://www.dovepress.com/long-non-coding-rna-tmpo-as1-promotes-cell-migration-and-invasion-by-s-peer-reviewed-article-CMAR |
| work_keys_str_mv | AT suny longnoncodingrnatmpoas1promotescellmigrationandinvasionbyspongingmir1405pandinducingsox4mediatedemtingastriccancer AT hanc longnoncodingrnatmpoas1promotescellmigrationandinvasionbyspongingmir1405pandinducingsox4mediatedemtingastriccancer |