Signalling-dependent adverse health effects of carbon nanoparticles are prevented by the compatible solute mannosylglycerate (firoin) in vitro and in vivo.
The inhalation of combustion-derived nanoparticles leads to adverse health effects in the airways. In this context the induction of membrane-coupled signalling is considered as causative for changes in tissue homeostasis and pro-inflammatory reactions. The identification of these molecular cell reac...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4240547?pdf=render |
| _version_ | 1818327532186894336 |
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| author | Andrea Autengruber Ulrich Sydlik Matthias Kroker Tamara Hornstein Niloofar Ale-Agha Daniel Stöckmann Andreas Bilstein Catrin Albrecht Adnana Paunel-Görgülü Christoph V Suschek Jean Krutmann Klaus Unfried |
| author_facet | Andrea Autengruber Ulrich Sydlik Matthias Kroker Tamara Hornstein Niloofar Ale-Agha Daniel Stöckmann Andreas Bilstein Catrin Albrecht Adnana Paunel-Görgülü Christoph V Suschek Jean Krutmann Klaus Unfried |
| author_sort | Andrea Autengruber |
| collection | DOAJ |
| description | The inhalation of combustion-derived nanoparticles leads to adverse health effects in the airways. In this context the induction of membrane-coupled signalling is considered as causative for changes in tissue homeostasis and pro-inflammatory reactions. The identification of these molecular cell reactions allowed to seek for strategies which interfere with these adverse effects. In the current study, we investigated the structurally different compatible solutes mannosylglycerate (firoin) from thermophilic bacteria and ectoine from halophilic bacteria for their capability to reduce signalling pathways triggered by carbon nanoparticles in target cells in the lung. The pre-treatment of lung epithelial cells with both substances decreased the particle-specific activation of mitogen-activated protein kinases and also the endpoints proliferation and apoptosis. Firoin applied into the lungs of animals, like ectoine, led to a significant reduction of the neutrophilic lung inflammation induced by particle exposure. The pro-inflammatory effect of carbon nanoparticles on human neutrophil granulocytes ex vivo was significantly reduced by both substances via the reduction of the anti-apoptotic membrane-dependent signalling. The data of this study together with earlier studies demonstrate that two structurally non-related compatible solutes are able to prevent pathogenic reactions of the airways to carbon nanoparticles by interfering with signalling events. The findings highlight the preventive or therapeutic potential of compatible solutes for adverse health effects caused by particle exposure of the airways. |
| first_indexed | 2024-12-13T12:17:46Z |
| format | Article |
| id | doaj.art-63c96b06ae024f998aac3624886e993f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T12:17:46Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-63c96b06ae024f998aac3624886e993f2022-12-21T23:46:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11148510.1371/journal.pone.0111485Signalling-dependent adverse health effects of carbon nanoparticles are prevented by the compatible solute mannosylglycerate (firoin) in vitro and in vivo.Andrea AutengruberUlrich SydlikMatthias KrokerTamara HornsteinNiloofar Ale-AghaDaniel StöckmannAndreas BilsteinCatrin AlbrechtAdnana Paunel-GörgülüChristoph V SuschekJean KrutmannKlaus UnfriedThe inhalation of combustion-derived nanoparticles leads to adverse health effects in the airways. In this context the induction of membrane-coupled signalling is considered as causative for changes in tissue homeostasis and pro-inflammatory reactions. The identification of these molecular cell reactions allowed to seek for strategies which interfere with these adverse effects. In the current study, we investigated the structurally different compatible solutes mannosylglycerate (firoin) from thermophilic bacteria and ectoine from halophilic bacteria for their capability to reduce signalling pathways triggered by carbon nanoparticles in target cells in the lung. The pre-treatment of lung epithelial cells with both substances decreased the particle-specific activation of mitogen-activated protein kinases and also the endpoints proliferation and apoptosis. Firoin applied into the lungs of animals, like ectoine, led to a significant reduction of the neutrophilic lung inflammation induced by particle exposure. The pro-inflammatory effect of carbon nanoparticles on human neutrophil granulocytes ex vivo was significantly reduced by both substances via the reduction of the anti-apoptotic membrane-dependent signalling. The data of this study together with earlier studies demonstrate that two structurally non-related compatible solutes are able to prevent pathogenic reactions of the airways to carbon nanoparticles by interfering with signalling events. The findings highlight the preventive or therapeutic potential of compatible solutes for adverse health effects caused by particle exposure of the airways.http://europepmc.org/articles/PMC4240547?pdf=render |
| spellingShingle | Andrea Autengruber Ulrich Sydlik Matthias Kroker Tamara Hornstein Niloofar Ale-Agha Daniel Stöckmann Andreas Bilstein Catrin Albrecht Adnana Paunel-Görgülü Christoph V Suschek Jean Krutmann Klaus Unfried Signalling-dependent adverse health effects of carbon nanoparticles are prevented by the compatible solute mannosylglycerate (firoin) in vitro and in vivo. PLoS ONE |
| title | Signalling-dependent adverse health effects of carbon nanoparticles are prevented by the compatible solute mannosylglycerate (firoin) in vitro and in vivo. |
| title_full | Signalling-dependent adverse health effects of carbon nanoparticles are prevented by the compatible solute mannosylglycerate (firoin) in vitro and in vivo. |
| title_fullStr | Signalling-dependent adverse health effects of carbon nanoparticles are prevented by the compatible solute mannosylglycerate (firoin) in vitro and in vivo. |
| title_full_unstemmed | Signalling-dependent adverse health effects of carbon nanoparticles are prevented by the compatible solute mannosylglycerate (firoin) in vitro and in vivo. |
| title_short | Signalling-dependent adverse health effects of carbon nanoparticles are prevented by the compatible solute mannosylglycerate (firoin) in vitro and in vivo. |
| title_sort | signalling dependent adverse health effects of carbon nanoparticles are prevented by the compatible solute mannosylglycerate firoin in vitro and in vivo |
| url | http://europepmc.org/articles/PMC4240547?pdf=render |
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