Regulation of the formyl peptide receptor 1 (FPR1) gene in primary human macrophages.
The formyl peptide receptor 1 (FPR1) is mainly expressed by mammalian phagocytic leukocytes and plays a role in chemotaxis, killing of microorganisms through phagocytosis, and the generation of reactive oxygen species. A large number of ligands have been identified triggering FPR1 including formylat...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3503994?pdf=render |
| _version_ | 1818021198273970176 |
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| author | Claudio Gemperle Mattia Schmid Magdalena Herova Jacqueline Marti-Jaun Sophia J A Wuest Christa Loretz Martin Hersberger |
| author_facet | Claudio Gemperle Mattia Schmid Magdalena Herova Jacqueline Marti-Jaun Sophia J A Wuest Christa Loretz Martin Hersberger |
| author_sort | Claudio Gemperle |
| collection | DOAJ |
| description | The formyl peptide receptor 1 (FPR1) is mainly expressed by mammalian phagocytic leukocytes and plays a role in chemotaxis, killing of microorganisms through phagocytosis, and the generation of reactive oxygen species. A large number of ligands have been identified triggering FPR1 including formylated and non-formylated peptides of microbial and endogenous origin. While the expression of FPR1 in neutrophils has been investigated intensively, knowledge on the regulation of FPR1 expression in polarized macrophages is lacking. In this study we show that primary human neutrophils, monocytes and resting macrophages do express the receptor on their cell surface. Polarization of macrophages with IFNγ, LPS and with the TLR8 ligand 3M-002 further increases FPR1 mRNA levels but does not consistently increase protein expression or chemotaxis towards the FPR1 ligand fMLF. In contrast, polarization of primary human macrophages with IL-4 and IL-13 leading to the alternative activated macrophages, reduces FPR1 cell surface expression and abolishes chemotaxis towards fMLF. These results show that M2 macrophages will not react to triggering of FPR1, limiting the role for FPR1 to chemotaxis and superoxide production of resting and pro-inflammatory M1 macrophages. |
| first_indexed | 2024-04-14T08:14:59Z |
| format | Article |
| id | doaj.art-63d1e33156444e07878039caab87000a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T08:14:59Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-63d1e33156444e07878039caab87000a2022-12-22T02:04:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01711e5019510.1371/journal.pone.0050195Regulation of the formyl peptide receptor 1 (FPR1) gene in primary human macrophages.Claudio GemperleMattia SchmidMagdalena HerovaJacqueline Marti-JaunSophia J A WuestChrista LoretzMartin HersbergerThe formyl peptide receptor 1 (FPR1) is mainly expressed by mammalian phagocytic leukocytes and plays a role in chemotaxis, killing of microorganisms through phagocytosis, and the generation of reactive oxygen species. A large number of ligands have been identified triggering FPR1 including formylated and non-formylated peptides of microbial and endogenous origin. While the expression of FPR1 in neutrophils has been investigated intensively, knowledge on the regulation of FPR1 expression in polarized macrophages is lacking. In this study we show that primary human neutrophils, monocytes and resting macrophages do express the receptor on their cell surface. Polarization of macrophages with IFNγ, LPS and with the TLR8 ligand 3M-002 further increases FPR1 mRNA levels but does not consistently increase protein expression or chemotaxis towards the FPR1 ligand fMLF. In contrast, polarization of primary human macrophages with IL-4 and IL-13 leading to the alternative activated macrophages, reduces FPR1 cell surface expression and abolishes chemotaxis towards fMLF. These results show that M2 macrophages will not react to triggering of FPR1, limiting the role for FPR1 to chemotaxis and superoxide production of resting and pro-inflammatory M1 macrophages.http://europepmc.org/articles/PMC3503994?pdf=render |
| spellingShingle | Claudio Gemperle Mattia Schmid Magdalena Herova Jacqueline Marti-Jaun Sophia J A Wuest Christa Loretz Martin Hersberger Regulation of the formyl peptide receptor 1 (FPR1) gene in primary human macrophages. PLoS ONE |
| title | Regulation of the formyl peptide receptor 1 (FPR1) gene in primary human macrophages. |
| title_full | Regulation of the formyl peptide receptor 1 (FPR1) gene in primary human macrophages. |
| title_fullStr | Regulation of the formyl peptide receptor 1 (FPR1) gene in primary human macrophages. |
| title_full_unstemmed | Regulation of the formyl peptide receptor 1 (FPR1) gene in primary human macrophages. |
| title_short | Regulation of the formyl peptide receptor 1 (FPR1) gene in primary human macrophages. |
| title_sort | regulation of the formyl peptide receptor 1 fpr1 gene in primary human macrophages |
| url | http://europepmc.org/articles/PMC3503994?pdf=render |
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