Development of Bioimpedance Sensors and Measurement System for Biomedical In-Vitro Applications
Bioimpedance analysis is a label-free and easy approach to obtain information on cellular barrier integrity and cell viability more broadly. In this work, we introduce a small, low-cost, portable in vitro impedance measurement system for studies where a shadow-free exposure of the cells is a require...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2021-10-01
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| Series: | Current Directions in Biomedical Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/cdbme-2021-2126 |
| _version_ | 1818013474086715392 |
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| author | Sebastian Stadler B. Eng. Plischke Herbert Hanshans Christian |
| author_facet | Sebastian Stadler B. Eng. Plischke Herbert Hanshans Christian |
| author_sort | Sebastian Stadler B. Eng. |
| collection | DOAJ |
| description | Bioimpedance analysis is a label-free and easy approach to obtain information on cellular barrier integrity and cell viability more broadly. In this work, we introduce a small, low-cost, portable in vitro impedance measurement system for studies where a shadow-free exposure of the cells is a requirement. It can be controlled by a user-friendly web interface and can perform measurements automated and autonomously at short intervals. The system can be integrated into an existing IoT network for remote monitoring and indepth analyses. A single-board computer (SBC) serves as the central unit, to control, analyze, store and forward the measurement data from the single-chip impedance analyzer. Various materials and manufacturing methods were used to produce a purpose-built lid on top of a modified 24-well microtiter plate in a “do it yourself” fashion. Furthermore, three different sensor designs were developed utilizing anodic aluminum oxide (AAO) membranes and gold-plated electrodes. Preliminary tests with potassium chloride (KCl) showed first promising results. |
| first_indexed | 2024-04-14T06:33:34Z |
| format | Article |
| id | doaj.art-63d98ce0b01b472693e4a79f329a339c |
| institution | Directory Open Access Journal |
| issn | 2364-5504 |
| language | English |
| last_indexed | 2024-04-14T06:33:34Z |
| publishDate | 2021-10-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Current Directions in Biomedical Engineering |
| spelling | doaj.art-63d98ce0b01b472693e4a79f329a339c2022-12-22T02:07:32ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042021-10-017249649910.1515/cdbme-2021-2126Development of Bioimpedance Sensors and Measurement System for Biomedical In-Vitro ApplicationsSebastian Stadler B. Eng.0Plischke Herbert1Hanshans Christian2Hochschule München University of Applied Sciences,Munich, GermanyHochschule München University of Applied Sciences,Munich, GermanyHochschule München University of Applied Sciences, Lothstraße 34,Munich, GermanyBioimpedance analysis is a label-free and easy approach to obtain information on cellular barrier integrity and cell viability more broadly. In this work, we introduce a small, low-cost, portable in vitro impedance measurement system for studies where a shadow-free exposure of the cells is a requirement. It can be controlled by a user-friendly web interface and can perform measurements automated and autonomously at short intervals. The system can be integrated into an existing IoT network for remote monitoring and indepth analyses. A single-board computer (SBC) serves as the central unit, to control, analyze, store and forward the measurement data from the single-chip impedance analyzer. Various materials and manufacturing methods were used to produce a purpose-built lid on top of a modified 24-well microtiter plate in a “do it yourself” fashion. Furthermore, three different sensor designs were developed utilizing anodic aluminum oxide (AAO) membranes and gold-plated electrodes. Preliminary tests with potassium chloride (KCl) showed first promising results.https://doi.org/10.1515/cdbme-2021-2126biosensingimpedance analysislabel-freeaao membranesbarrier integrityin vitro monitoring systemlight exposurecell growthapoptosis |
| spellingShingle | Sebastian Stadler B. Eng. Plischke Herbert Hanshans Christian Development of Bioimpedance Sensors and Measurement System for Biomedical In-Vitro Applications Current Directions in Biomedical Engineering biosensing impedance analysis label-free aao membranes barrier integrity in vitro monitoring system light exposure cell growth apoptosis |
| title | Development of Bioimpedance Sensors and Measurement System for Biomedical In-Vitro Applications |
| title_full | Development of Bioimpedance Sensors and Measurement System for Biomedical In-Vitro Applications |
| title_fullStr | Development of Bioimpedance Sensors and Measurement System for Biomedical In-Vitro Applications |
| title_full_unstemmed | Development of Bioimpedance Sensors and Measurement System for Biomedical In-Vitro Applications |
| title_short | Development of Bioimpedance Sensors and Measurement System for Biomedical In-Vitro Applications |
| title_sort | development of bioimpedance sensors and measurement system for biomedical in vitro applications |
| topic | biosensing impedance analysis label-free aao membranes barrier integrity in vitro monitoring system light exposure cell growth apoptosis |
| url | https://doi.org/10.1515/cdbme-2021-2126 |
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