Incidence of Campylobacter concisus and C. ureolyticus in traveler’s diarrhea cases and asymptomatic controls in Nepal and Thailand
Abstract Background Campylobacter concisus and C. ureolyticus have emerged in recent years as being associated with acute and prolonged gastroenteritis and implicated in the development of inflammatory bowel diseases. However, there are limited data on the prevalence of these microorganisms in South...
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| Format: | Article |
| Language: | English |
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BMC
2017-08-01
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| Series: | Gut Pathogens |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13099-017-0197-6 |
| _version_ | 1828338758612680704 |
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| author | Oralak Serichantalergs Sirigade Ruekit Prativa Pandey Sinn Anuras Carl Mason Ladaporn Bodhidatta Brett Swierczewski |
| author_facet | Oralak Serichantalergs Sirigade Ruekit Prativa Pandey Sinn Anuras Carl Mason Ladaporn Bodhidatta Brett Swierczewski |
| author_sort | Oralak Serichantalergs |
| collection | DOAJ |
| description | Abstract Background Campylobacter concisus and C. ureolyticus have emerged in recent years as being associated with acute and prolonged gastroenteritis and implicated in the development of inflammatory bowel diseases. However, there are limited data on the prevalence of these microorganisms in Southeast Asia. In this study, 214 pathogen-negative stool samples after laboratory examination for common enteric pathogens to include C. jejuni and C. coli by culture from two case–control traveler’s diarrhea (TD) studies conducted in Thailand (cases = 26; controls = 30) and Nepal (cases = 83; controls = 75) respectively were assayed by PCR for the detection of Campylobacter 16S rRNA and two specific heat shock protein genes specific for C. concisus (cpn60) and C. ureolyticus (Hsp60) respectively. Results Campylobacter 16S rRNA was detected in 28.5% (61/214) of the pathogen-negative TD stool samples (CIWEC Travel Medicine Clinic, Kathmandu, Nepal: cases = 36, control = 14; Bamrungrad International Hospital, Bangkok, Thailand: cases = 9, controls = 2). C. consisus was identified significantly more often in TD cases in Nepal (28.9%; 24/83) as compared to controls (4%; 3/75) (OR = 9.76; 95% CI 2.80–34.02; P = 0.0003) while C. consisus was detected in only two cases (2/26; 7.7%) and none of the controls stool samples from Thailand. C. ureolyticus was detected in four cases (4.8%; 4/83) and four controls (5.3%; 4/75) and in one case (3.8%; 1/26) and one control (3.1%; 1/30) from Nepal and Thailand respectively. C. jejuni and C. coli were isolated in 18.3 and 3.4% of the cases and in 4.0 and 1.4% of the controls in stool samples from both Thailand and Nepal respectively while C. concisus nor C. ureolyticus were not tested for in these samples. Conclusion These findings suggest that C. concisus potentially is a pathogen associated with TD in Nepal. To our knowledge, this is the first report of C. concisus and C. ureolyticus detected from traveler’s diarrhea cases from travelers to Nepal and Thailand. |
| first_indexed | 2024-04-13T22:33:25Z |
| format | Article |
| id | doaj.art-63dbf2a97b9441e0ae57313722f401ff |
| institution | Directory Open Access Journal |
| issn | 1757-4749 |
| language | English |
| last_indexed | 2024-04-13T22:33:25Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
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| series | Gut Pathogens |
| spelling | doaj.art-63dbf2a97b9441e0ae57313722f401ff2022-12-22T02:26:51ZengBMCGut Pathogens1757-47492017-08-01911710.1186/s13099-017-0197-6Incidence of Campylobacter concisus and C. ureolyticus in traveler’s diarrhea cases and asymptomatic controls in Nepal and ThailandOralak Serichantalergs0Sirigade Ruekit1Prativa Pandey2Sinn Anuras3Carl Mason4Ladaporn Bodhidatta5Brett Swierczewski6Department of Enteric Diseases, Armed Forces Research Institute of Medical SciencesDepartment of Enteric Diseases, Armed Forces Research Institute of Medical SciencesCIWEC Clinic Travel Medicine CenterBumrungrad International HospitalDepartment of Enteric Diseases, Armed Forces Research Institute of Medical SciencesDepartment of Enteric Diseases, Armed Forces Research Institute of Medical SciencesDepartment of Enteric Diseases, Armed Forces Research Institute of Medical SciencesAbstract Background Campylobacter concisus and C. ureolyticus have emerged in recent years as being associated with acute and prolonged gastroenteritis and implicated in the development of inflammatory bowel diseases. However, there are limited data on the prevalence of these microorganisms in Southeast Asia. In this study, 214 pathogen-negative stool samples after laboratory examination for common enteric pathogens to include C. jejuni and C. coli by culture from two case–control traveler’s diarrhea (TD) studies conducted in Thailand (cases = 26; controls = 30) and Nepal (cases = 83; controls = 75) respectively were assayed by PCR for the detection of Campylobacter 16S rRNA and two specific heat shock protein genes specific for C. concisus (cpn60) and C. ureolyticus (Hsp60) respectively. Results Campylobacter 16S rRNA was detected in 28.5% (61/214) of the pathogen-negative TD stool samples (CIWEC Travel Medicine Clinic, Kathmandu, Nepal: cases = 36, control = 14; Bamrungrad International Hospital, Bangkok, Thailand: cases = 9, controls = 2). C. consisus was identified significantly more often in TD cases in Nepal (28.9%; 24/83) as compared to controls (4%; 3/75) (OR = 9.76; 95% CI 2.80–34.02; P = 0.0003) while C. consisus was detected in only two cases (2/26; 7.7%) and none of the controls stool samples from Thailand. C. ureolyticus was detected in four cases (4.8%; 4/83) and four controls (5.3%; 4/75) and in one case (3.8%; 1/26) and one control (3.1%; 1/30) from Nepal and Thailand respectively. C. jejuni and C. coli were isolated in 18.3 and 3.4% of the cases and in 4.0 and 1.4% of the controls in stool samples from both Thailand and Nepal respectively while C. concisus nor C. ureolyticus were not tested for in these samples. Conclusion These findings suggest that C. concisus potentially is a pathogen associated with TD in Nepal. To our knowledge, this is the first report of C. concisus and C. ureolyticus detected from traveler’s diarrhea cases from travelers to Nepal and Thailand.http://link.springer.com/article/10.1186/s13099-017-0197-6CampylobacterTravelersDiarrheaNepalThailand |
| spellingShingle | Oralak Serichantalergs Sirigade Ruekit Prativa Pandey Sinn Anuras Carl Mason Ladaporn Bodhidatta Brett Swierczewski Incidence of Campylobacter concisus and C. ureolyticus in traveler’s diarrhea cases and asymptomatic controls in Nepal and Thailand Gut Pathogens Campylobacter Travelers Diarrhea Nepal Thailand |
| title | Incidence of Campylobacter concisus and C. ureolyticus in traveler’s diarrhea cases and asymptomatic controls in Nepal and Thailand |
| title_full | Incidence of Campylobacter concisus and C. ureolyticus in traveler’s diarrhea cases and asymptomatic controls in Nepal and Thailand |
| title_fullStr | Incidence of Campylobacter concisus and C. ureolyticus in traveler’s diarrhea cases and asymptomatic controls in Nepal and Thailand |
| title_full_unstemmed | Incidence of Campylobacter concisus and C. ureolyticus in traveler’s diarrhea cases and asymptomatic controls in Nepal and Thailand |
| title_short | Incidence of Campylobacter concisus and C. ureolyticus in traveler’s diarrhea cases and asymptomatic controls in Nepal and Thailand |
| title_sort | incidence of campylobacter concisus and c ureolyticus in traveler s diarrhea cases and asymptomatic controls in nepal and thailand |
| topic | Campylobacter Travelers Diarrhea Nepal Thailand |
| url | http://link.springer.com/article/10.1186/s13099-017-0197-6 |
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