Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken Embryos
The present study was conducted to investigate the effects of colloidal nanoparticles of silver (Nano-Ag) on the expression of myogenesis-related genes in chicken embryos. The investigated genes included the members of the myogenic regulatory factors family (<i>MRFs</i>) and myocyte enha...
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MDPI AG
2021-04-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/5/629 |
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| author | Walaa A. Husseiny Abeer A. I. Hassanin Adel A. S. El Nabtiti Karim Khalil Ahmed Elaswad |
| author_facet | Walaa A. Husseiny Abeer A. I. Hassanin Adel A. S. El Nabtiti Karim Khalil Ahmed Elaswad |
| author_sort | Walaa A. Husseiny |
| collection | DOAJ |
| description | The present study was conducted to investigate the effects of colloidal nanoparticles of silver (Nano-Ag) on the expression of myogenesis-related genes in chicken embryos. The investigated genes included the members of the myogenic regulatory factors family (<i>MRFs</i>) and myocyte enhancer factor 2A (<i>MEF2A</i>) genes. A total of 200 fertilized broiler eggs (Indian River) were randomly distributed into four groups; non-injected control, injected control with placebo, treatment I in ovo injected with 20 ppm Nano-Ag, and treatment II in ovo injected with 40 ppm Nano-Ag. The eggs were then incubated for 21 days at the optimum temperature and humidity conditions. Breast muscle tissues were collected at the 5th, 8th, and 18th days of the incubation period. The mRNA expression of myogenic determination factor 1 (<i>MYOD1</i>), myogenic factor 5 (<i>MYF5</i>), myogenic factor 6 (<i>MYF6</i>), myogenin (<i>MYOG</i>), and <i>MEF2A</i> was measured at the three sampling points using real-time quantitative PCR, while MYOD1 protein expression was evaluated on day 18 using western blot. Breast muscle tissues were histologically examined on day 18 to detect the changes at the cellular level. Our results indicate that myogenesis was enhanced with the low concentration (20 ppm) of Nano-Ag due to the higher expression of <i>MYOD1</i>, <i>MYF5</i>, and <i>MYF6</i> at the transcriptional level and <i>MYOD1</i> at the translational level. Moreover, histological analysis revealed the presence of hyperplasia (31.4% more muscle fibers) in treatment I (injected with 20 ppm). Our findings indicate that in ovo injection of 20 ppm Nano-Ag enhances the development of muscles in chicken embryos compared with the 40-ppm dosage and provide crucial information for the use of silver nanoparticles in poultry production. |
| first_indexed | 2024-03-10T12:03:46Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T12:03:46Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-63dc4da9b88348718c51110a2a2890b02023-11-21T16:43:56ZengMDPI AGGenes2073-44252021-04-0112562910.3390/genes12050629Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken EmbryosWalaa A. Husseiny0Abeer A. I. Hassanin1Adel A. S. El Nabtiti2Karim Khalil3Ahmed Elaswad4Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, EgyptAnatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, EgyptDepartment of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, EgyptThe present study was conducted to investigate the effects of colloidal nanoparticles of silver (Nano-Ag) on the expression of myogenesis-related genes in chicken embryos. The investigated genes included the members of the myogenic regulatory factors family (<i>MRFs</i>) and myocyte enhancer factor 2A (<i>MEF2A</i>) genes. A total of 200 fertilized broiler eggs (Indian River) were randomly distributed into four groups; non-injected control, injected control with placebo, treatment I in ovo injected with 20 ppm Nano-Ag, and treatment II in ovo injected with 40 ppm Nano-Ag. The eggs were then incubated for 21 days at the optimum temperature and humidity conditions. Breast muscle tissues were collected at the 5th, 8th, and 18th days of the incubation period. The mRNA expression of myogenic determination factor 1 (<i>MYOD1</i>), myogenic factor 5 (<i>MYF5</i>), myogenic factor 6 (<i>MYF6</i>), myogenin (<i>MYOG</i>), and <i>MEF2A</i> was measured at the three sampling points using real-time quantitative PCR, while MYOD1 protein expression was evaluated on day 18 using western blot. Breast muscle tissues were histologically examined on day 18 to detect the changes at the cellular level. Our results indicate that myogenesis was enhanced with the low concentration (20 ppm) of Nano-Ag due to the higher expression of <i>MYOD1</i>, <i>MYF5</i>, and <i>MYF6</i> at the transcriptional level and <i>MYOD1</i> at the translational level. Moreover, histological analysis revealed the presence of hyperplasia (31.4% more muscle fibers) in treatment I (injected with 20 ppm). Our findings indicate that in ovo injection of 20 ppm Nano-Ag enhances the development of muscles in chicken embryos compared with the 40-ppm dosage and provide crucial information for the use of silver nanoparticles in poultry production.https://www.mdpi.com/2073-4425/12/5/629myogenesis<i>MRFs</i>embryogenesisreal-time PCRnanoparticlesbroiler |
| spellingShingle | Walaa A. Husseiny Abeer A. I. Hassanin Adel A. S. El Nabtiti Karim Khalil Ahmed Elaswad Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken Embryos Genes myogenesis <i>MRFs</i> embryogenesis real-time PCR nanoparticles broiler |
| title | Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken Embryos |
| title_full | Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken Embryos |
| title_fullStr | Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken Embryos |
| title_full_unstemmed | Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken Embryos |
| title_short | Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken Embryos |
| title_sort | silver nanoparticles as modulators of myogenesis related gene expression in chicken embryos |
| topic | myogenesis <i>MRFs</i> embryogenesis real-time PCR nanoparticles broiler |
| url | https://www.mdpi.com/2073-4425/12/5/629 |
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