Bioassay-Guided Fractionation of <i>Erythrostemon yucatanensis</i> (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus
<i>Erythrostemon yucatanensis</i> (Greenm.) Gagnon & GP Lewis is a legume tree native to and widely distributed in southeast Mexico, where its branches are used in traditional medicine. An in vitro evaluation of the antiviral activity of extracts and fractions from the leaves, stem b...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/17/5494 |
| _version_ | 1797494178136981504 |
|---|---|
| author | Tania Ortiz-López Rocío Borges-Argáez Guadalupe Ayora-Talavera Ernesto Canto-Ramírez Lisseth Cetina-Montejo Ángel May-May Fabiola Escalante-Erosa Mirbella Cáceres-Farfán |
| author_facet | Tania Ortiz-López Rocío Borges-Argáez Guadalupe Ayora-Talavera Ernesto Canto-Ramírez Lisseth Cetina-Montejo Ángel May-May Fabiola Escalante-Erosa Mirbella Cáceres-Farfán |
| author_sort | Tania Ortiz-López |
| collection | DOAJ |
| description | <i>Erythrostemon yucatanensis</i> (Greenm.) Gagnon & GP Lewis is a legume tree native to and widely distributed in southeast Mexico, where its branches are used in traditional medicine. An in vitro evaluation of the antiviral activity of extracts and fractions from the leaves, stem bark and roots against two strains of the AH1N1 influenza virus was performed, leading to the identification of bioactive compounds in this medicinal plant. In a cytopathic effect reduction assay, the fractions from the leaves and stem bark were the active elements at the co-treatment level. These were further fractionated based on their hemagglutination inhibition activity. The analysis of spectroscopy data identified a combination of phytosterols (β-sitosterol, stigmasterol and campesterol) in the stem bark active fraction as the main anti-hemagglutinin binding components, while 5-hydroxy-2(2-hydroxy-3,4,5-trimethoxyphenyl)-7-metoxi-4H(chromen-4-ona), which was isolated from the leaf extracts, showed a weak inhibition of viral hemagglutinin. Time of addition experiments demonstrated that the mixture of sterols had a direct effect on viral particle infectivity at the co-treatment level (IC50 = 3.125 µg/mL). This effect was also observed in the virus plaque formation inhibition assay, where the mixture showed 90% inhibition in the first 20 min of co-treatment at the same concentration. Additionally, it was found using qRT-PCR that the NP copy number was reduced by 92.85% after 60 min of co-treatment. These results are the first report of components with anti-hemagglutinin binding activity in the genus <i>Erythrostemon</i> sp. |
| first_indexed | 2024-03-10T01:30:37Z |
| format | Article |
| id | doaj.art-63ddfff4ea6a481291f6e5de8fb799e6 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T01:30:37Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-63ddfff4ea6a481291f6e5de8fb799e62023-11-23T13:42:58ZengMDPI AGMolecules1420-30492022-08-012717549410.3390/molecules27175494Bioassay-Guided Fractionation of <i>Erythrostemon yucatanensis</i> (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 VirusTania Ortiz-López0Rocío Borges-Argáez1Guadalupe Ayora-Talavera2Ernesto Canto-Ramírez3Lisseth Cetina-Montejo4Ángel May-May5Fabiola Escalante-Erosa6Mirbella Cáceres-Farfán7Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Chuburná de Hidalgo, Mérida 97205, MexicoUnidad de Biotecnología, Centro de Investigación Científica de Yucatán, Chuburná de Hidalgo, Mérida 97205, MexicoDepartamento de Virología, Centro de Investigaciones Regionales, Universidad Autónoma de Yucatán, Paseo de Las Fuentes, Mérida 97225, MexicoIndependent Researchers, Mérida, Yucatán 97000, MexicoIndependent Researchers, Mérida, Yucatán 97000, MexicoIndependent Researchers, Mérida, Yucatán 97000, MexicoUnidad de Biotecnología, Centro de Investigación Científica de Yucatán, Chuburná de Hidalgo, Mérida 97205, MexicoUnidad de Biotecnología, Centro de Investigación Científica de Yucatán, Chuburná de Hidalgo, Mérida 97205, Mexico<i>Erythrostemon yucatanensis</i> (Greenm.) Gagnon & GP Lewis is a legume tree native to and widely distributed in southeast Mexico, where its branches are used in traditional medicine. An in vitro evaluation of the antiviral activity of extracts and fractions from the leaves, stem bark and roots against two strains of the AH1N1 influenza virus was performed, leading to the identification of bioactive compounds in this medicinal plant. In a cytopathic effect reduction assay, the fractions from the leaves and stem bark were the active elements at the co-treatment level. These were further fractionated based on their hemagglutination inhibition activity. The analysis of spectroscopy data identified a combination of phytosterols (β-sitosterol, stigmasterol and campesterol) in the stem bark active fraction as the main anti-hemagglutinin binding components, while 5-hydroxy-2(2-hydroxy-3,4,5-trimethoxyphenyl)-7-metoxi-4H(chromen-4-ona), which was isolated from the leaf extracts, showed a weak inhibition of viral hemagglutinin. Time of addition experiments demonstrated that the mixture of sterols had a direct effect on viral particle infectivity at the co-treatment level (IC50 = 3.125 µg/mL). This effect was also observed in the virus plaque formation inhibition assay, where the mixture showed 90% inhibition in the first 20 min of co-treatment at the same concentration. Additionally, it was found using qRT-PCR that the NP copy number was reduced by 92.85% after 60 min of co-treatment. These results are the first report of components with anti-hemagglutinin binding activity in the genus <i>Erythrostemon</i> sp.https://www.mdpi.com/1420-3049/27/17/5494<i>Erythrostemon yucatanensis</i>influenza A H1N1 virusanti-hemagglutininsterolsflavone |
| spellingShingle | Tania Ortiz-López Rocío Borges-Argáez Guadalupe Ayora-Talavera Ernesto Canto-Ramírez Lisseth Cetina-Montejo Ángel May-May Fabiola Escalante-Erosa Mirbella Cáceres-Farfán Bioassay-Guided Fractionation of <i>Erythrostemon yucatanensis</i> (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus Molecules <i>Erythrostemon yucatanensis</i> influenza A H1N1 virus anti-hemagglutinin sterols flavone |
| title | Bioassay-Guided Fractionation of <i>Erythrostemon yucatanensis</i> (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus |
| title_full | Bioassay-Guided Fractionation of <i>Erythrostemon yucatanensis</i> (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus |
| title_fullStr | Bioassay-Guided Fractionation of <i>Erythrostemon yucatanensis</i> (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus |
| title_full_unstemmed | Bioassay-Guided Fractionation of <i>Erythrostemon yucatanensis</i> (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus |
| title_short | Bioassay-Guided Fractionation of <i>Erythrostemon yucatanensis</i> (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus |
| title_sort | bioassay guided fractionation of i erythrostemon yucatanensis i greenm gagnon gp lewis components with anti hemagglutinin binding activity against influenza a h1n1 virus |
| topic | <i>Erythrostemon yucatanensis</i> influenza A H1N1 virus anti-hemagglutinin sterols flavone |
| url | https://www.mdpi.com/1420-3049/27/17/5494 |
| work_keys_str_mv | AT taniaortizlopez bioassayguidedfractionationofierythrostemonyucatanensisigreenmgagnongplewiscomponentswithantihemagglutininbindingactivityagainstinfluenzaah1n1virus AT rocioborgesargaez bioassayguidedfractionationofierythrostemonyucatanensisigreenmgagnongplewiscomponentswithantihemagglutininbindingactivityagainstinfluenzaah1n1virus AT guadalupeayoratalavera bioassayguidedfractionationofierythrostemonyucatanensisigreenmgagnongplewiscomponentswithantihemagglutininbindingactivityagainstinfluenzaah1n1virus AT ernestocantoramirez bioassayguidedfractionationofierythrostemonyucatanensisigreenmgagnongplewiscomponentswithantihemagglutininbindingactivityagainstinfluenzaah1n1virus AT lissethcetinamontejo bioassayguidedfractionationofierythrostemonyucatanensisigreenmgagnongplewiscomponentswithantihemagglutininbindingactivityagainstinfluenzaah1n1virus AT angelmaymay bioassayguidedfractionationofierythrostemonyucatanensisigreenmgagnongplewiscomponentswithantihemagglutininbindingactivityagainstinfluenzaah1n1virus AT fabiolaescalanteerosa bioassayguidedfractionationofierythrostemonyucatanensisigreenmgagnongplewiscomponentswithantihemagglutininbindingactivityagainstinfluenzaah1n1virus AT mirbellacaceresfarfan bioassayguidedfractionationofierythrostemonyucatanensisigreenmgagnongplewiscomponentswithantihemagglutininbindingactivityagainstinfluenzaah1n1virus |