| Summary: | This research aimed to use kiwi waste as fuel to generate bioelectricity through microbial fuel cells. It was possible to generate an electrical current and voltage peaks of 3.807 ± 0.102 mA and 0.993 ± 0.061 V on day 11, showing an electrical conductivity of 189.82 ± 3.029 mS/cm and an optimum operating pH of 5.966 ± 0.121. The internal resistance of the cells was calculated using Ohm’s Law, resulting in a value of 14.957 ± 0.394 Ω, while the maximum power density was 212.68 ± 26.84 mW/m<sup>2</sup> at a current density of 4.506 A/cm<sup>2</sup>. Through the analysis of the FTIR spectra carried out on the substrate, a decrease in the characteristic organic peaks was observed due to their decomposition during the electricity-generation process. In addition, it was possible to molecularly identify the bacteria <i>Comamonas testosteroni, Sphingobacterium</i> sp., and <i>Stenotropho-monas maltophila</i> adhered to the anodized biofilm. Finally, the capacity of this residue to generate bioelectricity was demonstrated by lighting an LED bulb with a voltage of 2.85 V.
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