Phylogenomic analyses and comparative genomics of Pseudomonas syringae associated with almond (Prunus dulcis) in California.
We sequenced and comprehensively analysed the genomic architecture of 98 fluorescent pseudomonads isolated from different symptomatic and asymptomatic tissues of almond and a few other Prunus spp. Phylogenomic analyses, genome mining, field pathogenicity tests, and in vitro ice nucleation and antibi...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0297867&type=printable |
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| author | Tawanda E Maguvu Rosa J Frias Alejandro I Hernandez-Rosas Brent A Holtz Franz J A Niederholzer Roger A Duncan Mohammad A Yaghmour Catherine M Culumber Phoebe E Gordon Flavia C F Vieira Philippe E Rolshausen James E Adaskaveg Lindsey P Burbank Steven E Lindow Florent P Trouillas |
| author_facet | Tawanda E Maguvu Rosa J Frias Alejandro I Hernandez-Rosas Brent A Holtz Franz J A Niederholzer Roger A Duncan Mohammad A Yaghmour Catherine M Culumber Phoebe E Gordon Flavia C F Vieira Philippe E Rolshausen James E Adaskaveg Lindsey P Burbank Steven E Lindow Florent P Trouillas |
| author_sort | Tawanda E Maguvu |
| collection | DOAJ |
| description | We sequenced and comprehensively analysed the genomic architecture of 98 fluorescent pseudomonads isolated from different symptomatic and asymptomatic tissues of almond and a few other Prunus spp. Phylogenomic analyses, genome mining, field pathogenicity tests, and in vitro ice nucleation and antibiotic sensitivity tests were integrated to improve knowledge of the biology and management of bacterial blast and bacterial canker of almond. We identified Pseudomonas syringae pv. syringae, P. cerasi, and P. viridiflava as almond canker pathogens. P. syringae pv. syringae caused both canker and foliar (blast) symptoms. In contrast, P. cerasi and P. viridiflava only caused cankers, and P. viridiflava appeared to be a weak pathogen of almond. Isolates belonging to P. syringae pv. syringae were the most frequently isolated among the pathogenic species/pathovars, composing 75% of all pathogenic isolates. P. cerasi and P. viridiflava isolates composed 8.3 and 16.7% of the pathogenic isolates, respectively. Laboratory leaf infiltration bioassays produced results distinct from experiments in the field with both P. cerasi and P. syringae pv. syringae, causing significant necrosis and browning of detached leaves, whereas P. viridiflava conferred moderate effects. Genome mining revealed the absence of key epiphytic fitness-related genes in P. cerasi and P. viridiflava genomic sequences, which could explain the contrasting field and laboratory bioassay results. P. syringae pv. syringae and P. cerasi isolates harboured the ice nucleation protein, which correlated with the ice nucleation phenotype. Results of sensitivity tests to copper and kasugamycin showed a strong linkage to putative resistance genes. Isolates harbouring the ctpV gene showed resistance to copper up to 600 μg/ml. In contrast, isolates without the ctpV gene could not grow on nutrient agar amended with 200 μg/ml copper, suggesting ctpV can be used to phenotype copper resistance. All isolates were sensitive to kasugamycin at the label-recommended rate of 100μg/ml. |
| first_indexed | 2024-04-24T07:57:07Z |
| format | Article |
| id | doaj.art-63e22afe149f4754a5848cc8ed6a80fb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-24T07:57:07Z |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-63e22afe149f4754a5848cc8ed6a80fb2024-04-18T05:31:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01194e029786710.1371/journal.pone.0297867Phylogenomic analyses and comparative genomics of Pseudomonas syringae associated with almond (Prunus dulcis) in California.Tawanda E MaguvuRosa J FriasAlejandro I Hernandez-RosasBrent A HoltzFranz J A NiederholzerRoger A DuncanMohammad A YaghmourCatherine M CulumberPhoebe E GordonFlavia C F VieiraPhilippe E RolshausenJames E AdaskavegLindsey P BurbankSteven E LindowFlorent P TrouillasWe sequenced and comprehensively analysed the genomic architecture of 98 fluorescent pseudomonads isolated from different symptomatic and asymptomatic tissues of almond and a few other Prunus spp. Phylogenomic analyses, genome mining, field pathogenicity tests, and in vitro ice nucleation and antibiotic sensitivity tests were integrated to improve knowledge of the biology and management of bacterial blast and bacterial canker of almond. We identified Pseudomonas syringae pv. syringae, P. cerasi, and P. viridiflava as almond canker pathogens. P. syringae pv. syringae caused both canker and foliar (blast) symptoms. In contrast, P. cerasi and P. viridiflava only caused cankers, and P. viridiflava appeared to be a weak pathogen of almond. Isolates belonging to P. syringae pv. syringae were the most frequently isolated among the pathogenic species/pathovars, composing 75% of all pathogenic isolates. P. cerasi and P. viridiflava isolates composed 8.3 and 16.7% of the pathogenic isolates, respectively. Laboratory leaf infiltration bioassays produced results distinct from experiments in the field with both P. cerasi and P. syringae pv. syringae, causing significant necrosis and browning of detached leaves, whereas P. viridiflava conferred moderate effects. Genome mining revealed the absence of key epiphytic fitness-related genes in P. cerasi and P. viridiflava genomic sequences, which could explain the contrasting field and laboratory bioassay results. P. syringae pv. syringae and P. cerasi isolates harboured the ice nucleation protein, which correlated with the ice nucleation phenotype. Results of sensitivity tests to copper and kasugamycin showed a strong linkage to putative resistance genes. Isolates harbouring the ctpV gene showed resistance to copper up to 600 μg/ml. In contrast, isolates without the ctpV gene could not grow on nutrient agar amended with 200 μg/ml copper, suggesting ctpV can be used to phenotype copper resistance. All isolates were sensitive to kasugamycin at the label-recommended rate of 100μg/ml.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0297867&type=printable |
| spellingShingle | Tawanda E Maguvu Rosa J Frias Alejandro I Hernandez-Rosas Brent A Holtz Franz J A Niederholzer Roger A Duncan Mohammad A Yaghmour Catherine M Culumber Phoebe E Gordon Flavia C F Vieira Philippe E Rolshausen James E Adaskaveg Lindsey P Burbank Steven E Lindow Florent P Trouillas Phylogenomic analyses and comparative genomics of Pseudomonas syringae associated with almond (Prunus dulcis) in California. PLoS ONE |
| title | Phylogenomic analyses and comparative genomics of Pseudomonas syringae associated with almond (Prunus dulcis) in California. |
| title_full | Phylogenomic analyses and comparative genomics of Pseudomonas syringae associated with almond (Prunus dulcis) in California. |
| title_fullStr | Phylogenomic analyses and comparative genomics of Pseudomonas syringae associated with almond (Prunus dulcis) in California. |
| title_full_unstemmed | Phylogenomic analyses and comparative genomics of Pseudomonas syringae associated with almond (Prunus dulcis) in California. |
| title_short | Phylogenomic analyses and comparative genomics of Pseudomonas syringae associated with almond (Prunus dulcis) in California. |
| title_sort | phylogenomic analyses and comparative genomics of pseudomonas syringae associated with almond prunus dulcis in california |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0297867&type=printable |
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