Injectable biocomposites for bone healing in rabbit femoral condyle defects.
A novel biomimetic bone scaffold was successfully prepared in this study, which was composed of calcium sulfate hemihydrate (CSH), collagen and nano-hydroxyapatite (nHAC). CSH/nHAC was prepared and observed with scanning electron microscope and rhBMP-2 was introduced into CSH/nHAC. The released prot...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3797737?pdf=render |
| _version_ | 1818926799400206336 |
|---|---|
| author | Jianheng Liu Kezheng Mao Zhengsheng Liu Xiumei Wang Fuzhai Cui Wenguang Guo Keya Mao Shuying Yang |
| author_facet | Jianheng Liu Kezheng Mao Zhengsheng Liu Xiumei Wang Fuzhai Cui Wenguang Guo Keya Mao Shuying Yang |
| author_sort | Jianheng Liu |
| collection | DOAJ |
| description | A novel biomimetic bone scaffold was successfully prepared in this study, which was composed of calcium sulfate hemihydrate (CSH), collagen and nano-hydroxyapatite (nHAC). CSH/nHAC was prepared and observed with scanning electron microscope and rhBMP-2 was introduced into CSH/nHAC. The released protein content from the scaffold was detected using high performance liquid chromatography at predetermined time interval. In vivo bone formation capacity was investigated by means of implanting the scaffolds with rhBMP-2 or without rhBMP-2 respectively into a critical size defect model in the femoral condyle of rabbit. The releasing character of rhBMP-2 was that an initial burst release (37.5%) was observed in the first day, followed by a sustained release and reached 100% at the end of day 20. The CSH/nHAC showed a gradual decrease in degradation with the content of nHAC increase. The results of X-rays, Micro CT and histological observation indicated that more new bone was formed in rhBMP-2 group. The results implied that this new injectable bone scaffold should be very promising for bone repair and has a great potential in bone tissue engineering. |
| first_indexed | 2024-12-20T03:02:51Z |
| format | Article |
| id | doaj.art-63e27a0c64574c6db14f73ccdd4b894f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T03:02:51Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-63e27a0c64574c6db14f73ccdd4b894f2022-12-21T19:55:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7566810.1371/journal.pone.0075668Injectable biocomposites for bone healing in rabbit femoral condyle defects.Jianheng LiuKezheng MaoZhengsheng LiuXiumei WangFuzhai CuiWenguang GuoKeya MaoShuying YangA novel biomimetic bone scaffold was successfully prepared in this study, which was composed of calcium sulfate hemihydrate (CSH), collagen and nano-hydroxyapatite (nHAC). CSH/nHAC was prepared and observed with scanning electron microscope and rhBMP-2 was introduced into CSH/nHAC. The released protein content from the scaffold was detected using high performance liquid chromatography at predetermined time interval. In vivo bone formation capacity was investigated by means of implanting the scaffolds with rhBMP-2 or without rhBMP-2 respectively into a critical size defect model in the femoral condyle of rabbit. The releasing character of rhBMP-2 was that an initial burst release (37.5%) was observed in the first day, followed by a sustained release and reached 100% at the end of day 20. The CSH/nHAC showed a gradual decrease in degradation with the content of nHAC increase. The results of X-rays, Micro CT and histological observation indicated that more new bone was formed in rhBMP-2 group. The results implied that this new injectable bone scaffold should be very promising for bone repair and has a great potential in bone tissue engineering.http://europepmc.org/articles/PMC3797737?pdf=render |
| spellingShingle | Jianheng Liu Kezheng Mao Zhengsheng Liu Xiumei Wang Fuzhai Cui Wenguang Guo Keya Mao Shuying Yang Injectable biocomposites for bone healing in rabbit femoral condyle defects. PLoS ONE |
| title | Injectable biocomposites for bone healing in rabbit femoral condyle defects. |
| title_full | Injectable biocomposites for bone healing in rabbit femoral condyle defects. |
| title_fullStr | Injectable biocomposites for bone healing in rabbit femoral condyle defects. |
| title_full_unstemmed | Injectable biocomposites for bone healing in rabbit femoral condyle defects. |
| title_short | Injectable biocomposites for bone healing in rabbit femoral condyle defects. |
| title_sort | injectable biocomposites for bone healing in rabbit femoral condyle defects |
| url | http://europepmc.org/articles/PMC3797737?pdf=render |
| work_keys_str_mv | AT jianhengliu injectablebiocompositesforbonehealinginrabbitfemoralcondyledefects AT kezhengmao injectablebiocompositesforbonehealinginrabbitfemoralcondyledefects AT zhengshengliu injectablebiocompositesforbonehealinginrabbitfemoralcondyledefects AT xiumeiwang injectablebiocompositesforbonehealinginrabbitfemoralcondyledefects AT fuzhaicui injectablebiocompositesforbonehealinginrabbitfemoralcondyledefects AT wenguangguo injectablebiocompositesforbonehealinginrabbitfemoralcondyledefects AT keyamao injectablebiocompositesforbonehealinginrabbitfemoralcondyledefects AT shuyingyang injectablebiocompositesforbonehealinginrabbitfemoralcondyledefects |