Genomes and virulence factors of novel bacterial pathogens causing bleaching disease in the marine red alga Delisea pulchra.
Nautella sp. R11, a member of the marine Roseobacter clade, causes a bleaching disease in the temperate-marine red macroalga, Delisea pulchra. To begin to elucidate the molecular mechanisms underpinning the ability of Nautella sp. R11 to colonize, invade and induce bleaching of D. pulchra, we sequen...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3230580?pdf=render |
| _version_ | 1811327551875842048 |
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| author | Neil Fernandes Rebecca J Case Sharon R Longford Mohammad R Seyedsayamdost Peter D Steinberg Staffan Kjelleberg Torsten Thomas |
| author_facet | Neil Fernandes Rebecca J Case Sharon R Longford Mohammad R Seyedsayamdost Peter D Steinberg Staffan Kjelleberg Torsten Thomas |
| author_sort | Neil Fernandes |
| collection | DOAJ |
| description | Nautella sp. R11, a member of the marine Roseobacter clade, causes a bleaching disease in the temperate-marine red macroalga, Delisea pulchra. To begin to elucidate the molecular mechanisms underpinning the ability of Nautella sp. R11 to colonize, invade and induce bleaching of D. pulchra, we sequenced and analyzed its genome. The genome encodes several factors such as adhesion mechanisms, systems for the transport of algal metabolites, enzymes that confer resistance to oxidative stress, cytolysins, and global regulatory mechanisms that may allow for the switch of Nautella sp. R11 to a pathogenic lifestyle. Many virulence effectors common in phytopathogenic bacteria are also found in the R11 genome, such as the plant hormone indole acetic acid, cellulose fibrils, succinoglycan and nodulation protein L. Comparative genomics with non-pathogenic Roseobacter strains and a newly identified pathogen, Phaeobacter sp. LSS9, revealed a patchy distribution of putative virulence factors in all genomes, but also led to the identification of a quorum sensing (QS) dependent transcriptional regulator that was unique to pathogenic Roseobacter strains. This observation supports the model that a combination of virulence factors and QS-dependent regulatory mechanisms enables indigenous members of the host alga's epiphytic microbial community to switch to a pathogenic lifestyle, especially under environmental conditions when innate host defence mechanisms are compromised. |
| first_indexed | 2024-04-13T15:08:49Z |
| format | Article |
| id | doaj.art-63e6f96d51b541dfb7d2fec5fb75ba96 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T15:08:49Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-63e6f96d51b541dfb7d2fec5fb75ba962022-12-22T02:42:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01612e2738710.1371/journal.pone.0027387Genomes and virulence factors of novel bacterial pathogens causing bleaching disease in the marine red alga Delisea pulchra.Neil FernandesRebecca J CaseSharon R LongfordMohammad R SeyedsayamdostPeter D SteinbergStaffan KjellebergTorsten ThomasNautella sp. R11, a member of the marine Roseobacter clade, causes a bleaching disease in the temperate-marine red macroalga, Delisea pulchra. To begin to elucidate the molecular mechanisms underpinning the ability of Nautella sp. R11 to colonize, invade and induce bleaching of D. pulchra, we sequenced and analyzed its genome. The genome encodes several factors such as adhesion mechanisms, systems for the transport of algal metabolites, enzymes that confer resistance to oxidative stress, cytolysins, and global regulatory mechanisms that may allow for the switch of Nautella sp. R11 to a pathogenic lifestyle. Many virulence effectors common in phytopathogenic bacteria are also found in the R11 genome, such as the plant hormone indole acetic acid, cellulose fibrils, succinoglycan and nodulation protein L. Comparative genomics with non-pathogenic Roseobacter strains and a newly identified pathogen, Phaeobacter sp. LSS9, revealed a patchy distribution of putative virulence factors in all genomes, but also led to the identification of a quorum sensing (QS) dependent transcriptional regulator that was unique to pathogenic Roseobacter strains. This observation supports the model that a combination of virulence factors and QS-dependent regulatory mechanisms enables indigenous members of the host alga's epiphytic microbial community to switch to a pathogenic lifestyle, especially under environmental conditions when innate host defence mechanisms are compromised.http://europepmc.org/articles/PMC3230580?pdf=render |
| spellingShingle | Neil Fernandes Rebecca J Case Sharon R Longford Mohammad R Seyedsayamdost Peter D Steinberg Staffan Kjelleberg Torsten Thomas Genomes and virulence factors of novel bacterial pathogens causing bleaching disease in the marine red alga Delisea pulchra. PLoS ONE |
| title | Genomes and virulence factors of novel bacterial pathogens causing bleaching disease in the marine red alga Delisea pulchra. |
| title_full | Genomes and virulence factors of novel bacterial pathogens causing bleaching disease in the marine red alga Delisea pulchra. |
| title_fullStr | Genomes and virulence factors of novel bacterial pathogens causing bleaching disease in the marine red alga Delisea pulchra. |
| title_full_unstemmed | Genomes and virulence factors of novel bacterial pathogens causing bleaching disease in the marine red alga Delisea pulchra. |
| title_short | Genomes and virulence factors of novel bacterial pathogens causing bleaching disease in the marine red alga Delisea pulchra. |
| title_sort | genomes and virulence factors of novel bacterial pathogens causing bleaching disease in the marine red alga delisea pulchra |
| url | http://europepmc.org/articles/PMC3230580?pdf=render |
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