Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1
The aim of this study was to investigate the effect of Bifidobacterium lactis Probio-M8 on glucolipid metabolism and gut microbiota (GM) composition in type 2 diabetes mellitus (T2DM) mice. The glucolipid metabolic profiles were analyzed. The 16S rRNA gene sequencing was employed to investigate GM....
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2022-12-01
|
| Series: | Open Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/med-2022-0576 |
| _version_ | 1797947750363430912 |
|---|---|
| author | Chen Ye Zhao Yaxin Shen Xin Zhao Feiyan Qi Jinxin Zhong Zhi Li Dongmei |
| author_facet | Chen Ye Zhao Yaxin Shen Xin Zhao Feiyan Qi Jinxin Zhong Zhi Li Dongmei |
| author_sort | Chen Ye |
| collection | DOAJ |
| description | The aim of this study was to investigate the effect of Bifidobacterium lactis Probio-M8 on glucolipid metabolism and gut microbiota (GM) composition in type 2 diabetes mellitus (T2DM) mice. The glucolipid metabolic profiles were analyzed. The 16S rRNA gene sequencing was employed to investigate GM. The levels of farnesyl X receptor (FXR) and cytochrome p450 7A1 (CYP7A1) were detected by quantitative polymerase chain reaction and western blot assays. The total bile acids (TBAs), ceramide (CE), glucagon-like peptide-1 (GLP-1), and fibroblast growth factor (FGF)-15 were also detected. The morphological features of liver and pancreas were also analyzed. Compared with the model group, Probio-M8 restored body weight, food intake and water intake, as well as improved hyperglycemia symptoms, serum glucolipid parameters, and the composition of intestinal microbes in T2DM diabetic mice. Moreover, the reduced level of FXR and the increased level of CYP7A1 in T2DM mice were reversed by Probio-M8 treatment. The increased levels of TBA and CE and the reduced levels of GLP-1 and FGF-15 in T2DM mice were altered after Probio-M8 stimulation. Besides, the altered morphology of liver and ileum in T2DM mice was alleviated by Probio-M8 treatment. Taken together, we suggested that the symptoms of T2DM could be ameliorated by Probio-M8 in T2DM mice. |
| first_indexed | 2024-04-10T21:32:38Z |
| format | Article |
| id | doaj.art-63e71bae79264bf7baeadcecf7d45956 |
| institution | Directory Open Access Journal |
| issn | 2391-5463 |
| language | English |
| last_indexed | 2024-04-10T21:32:38Z |
| publishDate | 2022-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Medicine |
| spelling | doaj.art-63e71bae79264bf7baeadcecf7d459562023-01-19T13:04:57ZengDe GruyterOpen Medicine2391-54632022-12-011712072208410.1515/med-2022-0576Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1Chen Ye0Zhao Yaxin1Shen Xin2Zhao Feiyan3Qi Jinxin4Zhong Zhi5Li Dongmei6Department of Endocrinology, Inner Mongolia People’s Hospital, Hohhot, 010017, Inner Mongolia, P. R. ChinaDepartment of Endocrinology, Inner Mongolia People’s Hospital, Hohhot, 010017, Inner Mongolia, P. R. ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, Inner Mongolia, P. R. ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, Inner Mongolia, P. R. ChinaDepartment of Rheumatology and Immunology, Bayannur Hospital, Bayannur, 015000, Inner Mongolia, P. R. ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, Inner Mongolia, P. R. ChinaDepartment of Endocrinology, Inner Mongolia People’s Hospital, Hohhot, 010017, Inner Mongolia, P. R. ChinaThe aim of this study was to investigate the effect of Bifidobacterium lactis Probio-M8 on glucolipid metabolism and gut microbiota (GM) composition in type 2 diabetes mellitus (T2DM) mice. The glucolipid metabolic profiles were analyzed. The 16S rRNA gene sequencing was employed to investigate GM. The levels of farnesyl X receptor (FXR) and cytochrome p450 7A1 (CYP7A1) were detected by quantitative polymerase chain reaction and western blot assays. The total bile acids (TBAs), ceramide (CE), glucagon-like peptide-1 (GLP-1), and fibroblast growth factor (FGF)-15 were also detected. The morphological features of liver and pancreas were also analyzed. Compared with the model group, Probio-M8 restored body weight, food intake and water intake, as well as improved hyperglycemia symptoms, serum glucolipid parameters, and the composition of intestinal microbes in T2DM diabetic mice. Moreover, the reduced level of FXR and the increased level of CYP7A1 in T2DM mice were reversed by Probio-M8 treatment. The increased levels of TBA and CE and the reduced levels of GLP-1 and FGF-15 in T2DM mice were altered after Probio-M8 stimulation. Besides, the altered morphology of liver and ileum in T2DM mice was alleviated by Probio-M8 treatment. Taken together, we suggested that the symptoms of T2DM could be ameliorated by Probio-M8 in T2DM mice.https://doi.org/10.1515/med-2022-0576probio-m8type 2 diabetes mellitusfxr-cyp7a1glucolipid metabolismgut microbiota |
| spellingShingle | Chen Ye Zhao Yaxin Shen Xin Zhao Feiyan Qi Jinxin Zhong Zhi Li Dongmei Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1 Open Medicine probio-m8 type 2 diabetes mellitus fxr-cyp7a1 glucolipid metabolism gut microbiota |
| title | Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1 |
| title_full | Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1 |
| title_fullStr | Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1 |
| title_full_unstemmed | Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1 |
| title_short | Bifidobacterium lactis Probio-M8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum FXR-CYP7A1 |
| title_sort | bifidobacterium lactis probio m8 ameliorated the symptoms of type 2 diabetes mellitus mice by changing ileum fxr cyp7a1 |
| topic | probio-m8 type 2 diabetes mellitus fxr-cyp7a1 glucolipid metabolism gut microbiota |
| url | https://doi.org/10.1515/med-2022-0576 |
| work_keys_str_mv | AT chenye bifidobacteriumlactisprobiom8amelioratedthesymptomsoftype2diabetesmellitusmicebychangingileumfxrcyp7a1 AT zhaoyaxin bifidobacteriumlactisprobiom8amelioratedthesymptomsoftype2diabetesmellitusmicebychangingileumfxrcyp7a1 AT shenxin bifidobacteriumlactisprobiom8amelioratedthesymptomsoftype2diabetesmellitusmicebychangingileumfxrcyp7a1 AT zhaofeiyan bifidobacteriumlactisprobiom8amelioratedthesymptomsoftype2diabetesmellitusmicebychangingileumfxrcyp7a1 AT qijinxin bifidobacteriumlactisprobiom8amelioratedthesymptomsoftype2diabetesmellitusmicebychangingileumfxrcyp7a1 AT zhongzhi bifidobacteriumlactisprobiom8amelioratedthesymptomsoftype2diabetesmellitusmicebychangingileumfxrcyp7a1 AT lidongmei bifidobacteriumlactisprobiom8amelioratedthesymptomsoftype2diabetesmellitusmicebychangingileumfxrcyp7a1 |