T cell contamination in flow cytometry gating approaches for analysis of innate lymphoid cells.
Innate lymphoid cells (ILCs) differ from T and B cells as they do not express genetically rearranged antigen receptors. The most prominent member of this group, NK cells, can be identified by numerous surface receptors such as natural cytotoxicity receptors (NCRs). However, novel groups of ILCs have...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0094196&type=printable |
| _version_ | 1826587043132604416 |
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| author | Sara H Burkhard Florian Mair Kathrin Nussbaum Sabrina Hasler Burkhard Becher |
| author_facet | Sara H Burkhard Florian Mair Kathrin Nussbaum Sabrina Hasler Burkhard Becher |
| author_sort | Sara H Burkhard |
| collection | DOAJ |
| description | Innate lymphoid cells (ILCs) differ from T and B cells as they do not express genetically rearranged antigen receptors. The most prominent member of this group, NK cells, can be identified by numerous surface receptors such as natural cytotoxicity receptors (NCRs). However, novel groups of ILCs have recently been described and classified based on fate-determining transcription factors and cytokines being produced, similarly to T helper cells. Due to the lack of exclusive markers, ILCs are primarily defined by the paucity of lineage markers. Using RORc-fate-mapping mice, we found that the common lineage exclusion using CD3 yields an ILC population containing a large proportion of T cells with recombined TCR loci and low expression of CD3. Thus, we suggest adding CD5 as a marker for thorough elimination of T cells to avoid erroneous interpretations of ILC function in immunity. |
| first_indexed | 2024-12-23T11:21:39Z |
| format | Article |
| id | doaj.art-63e8d9406e1341e683fb3e2df5a768a9 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T16:19:09Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-63e8d9406e1341e683fb3e2df5a768a92025-02-22T05:34:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9419610.1371/journal.pone.0094196T cell contamination in flow cytometry gating approaches for analysis of innate lymphoid cells.Sara H BurkhardFlorian MairKathrin NussbaumSabrina HaslerBurkhard BecherInnate lymphoid cells (ILCs) differ from T and B cells as they do not express genetically rearranged antigen receptors. The most prominent member of this group, NK cells, can be identified by numerous surface receptors such as natural cytotoxicity receptors (NCRs). However, novel groups of ILCs have recently been described and classified based on fate-determining transcription factors and cytokines being produced, similarly to T helper cells. Due to the lack of exclusive markers, ILCs are primarily defined by the paucity of lineage markers. Using RORc-fate-mapping mice, we found that the common lineage exclusion using CD3 yields an ILC population containing a large proportion of T cells with recombined TCR loci and low expression of CD3. Thus, we suggest adding CD5 as a marker for thorough elimination of T cells to avoid erroneous interpretations of ILC function in immunity.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0094196&type=printable |
| spellingShingle | Sara H Burkhard Florian Mair Kathrin Nussbaum Sabrina Hasler Burkhard Becher T cell contamination in flow cytometry gating approaches for analysis of innate lymphoid cells. PLoS ONE |
| title | T cell contamination in flow cytometry gating approaches for analysis of innate lymphoid cells. |
| title_full | T cell contamination in flow cytometry gating approaches for analysis of innate lymphoid cells. |
| title_fullStr | T cell contamination in flow cytometry gating approaches for analysis of innate lymphoid cells. |
| title_full_unstemmed | T cell contamination in flow cytometry gating approaches for analysis of innate lymphoid cells. |
| title_short | T cell contamination in flow cytometry gating approaches for analysis of innate lymphoid cells. |
| title_sort | t cell contamination in flow cytometry gating approaches for analysis of innate lymphoid cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0094196&type=printable |
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