Annotation and validation of genes involved in photosynthesis and starch synthesis from a Manihot full-length cDNA library
A full-length cDNA library from leaf and root tissues of cassava (Manihot esculenta) Arg7 and one accession of its wild ancestor W14 (M. esculenta ssp. flabellifolia) has been constructed. The library is comprised of four sub-libraries, containing 32640 recombinant clones, 6028 cDNA clones from thei...
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| Format: | Article |
| Language: | English |
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Higher Education Press
2016-12-01
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| Series: | Frontiers of Agricultural Science and Engineering |
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| Online Access: | http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/1477362188366-1648564722.pdf |
| _version_ | 1818489071495806976 |
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| author | Yang ZHANG,Xin CHEN,Haiyan WANG,Zhiqiang XIA,Peng LING,Wenquan WANG |
| author_facet | Yang ZHANG,Xin CHEN,Haiyan WANG,Zhiqiang XIA,Peng LING,Wenquan WANG |
| author_sort | Yang ZHANG,Xin CHEN,Haiyan WANG,Zhiqiang XIA,Peng LING,Wenquan WANG |
| collection | DOAJ |
| description | A full-length cDNA library from leaf and root tissues of cassava (Manihot esculenta) Arg7 and one accession of its wild ancestor W14 (M. esculenta ssp. flabellifolia) has been constructed. The library is comprised of four sub-libraries, containing 32640 recombinant clones, 6028 cDNA clones from their 5′ ends, and 128 clones from the 3′ ends were sequenced. In total, 5013 high-quality expressed sequence tags (ESTs) and 1259 unigenes were obtained. Of these, 746 unigenes were identified by their sequence homologies to ESTs from model plants, and 323 unigenes were mapped onto 114 different KEGG pathways. From these, 24 differentially expressed genes involved in starch metabolism and photosynthesis were identified and five of them were selected to compare their expression level between Arg7 and W14. Notably, Arg7 has a higher net photosynthesis rate in leaves, higher ribulose-1,5-bisphosphate carboxy-lase oxygenase activities in leaves, and higher AGPase activity in roots. This resource is the first EST collection from wild cassava and should be of value for gene discovery, genome annotation and studies of Manihot evolution. |
| first_indexed | 2024-12-10T16:58:50Z |
| format | Article |
| id | doaj.art-63ed5579462443498976db8984258409 |
| institution | Directory Open Access Journal |
| issn | 2095-7505 |
| language | English |
| last_indexed | 2024-12-10T16:58:50Z |
| publishDate | 2016-12-01 |
| publisher | Higher Education Press |
| record_format | Article |
| series | Frontiers of Agricultural Science and Engineering |
| spelling | doaj.art-63ed5579462443498976db89842584092022-12-22T01:40:37ZengHigher Education PressFrontiers of Agricultural Science and Engineering2095-75052016-12-013430832010.15302/J-FASE-2016113Annotation and validation of genes involved in photosynthesis and starch synthesis from a Manihot full-length cDNA libraryYang ZHANG,Xin CHEN,Haiyan WANG,Zhiqiang XIA,Peng LING,Wenquan WANG01. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; 2. Department of Horticultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USAA full-length cDNA library from leaf and root tissues of cassava (Manihot esculenta) Arg7 and one accession of its wild ancestor W14 (M. esculenta ssp. flabellifolia) has been constructed. The library is comprised of four sub-libraries, containing 32640 recombinant clones, 6028 cDNA clones from their 5′ ends, and 128 clones from the 3′ ends were sequenced. In total, 5013 high-quality expressed sequence tags (ESTs) and 1259 unigenes were obtained. Of these, 746 unigenes were identified by their sequence homologies to ESTs from model plants, and 323 unigenes were mapped onto 114 different KEGG pathways. From these, 24 differentially expressed genes involved in starch metabolism and photosynthesis were identified and five of them were selected to compare their expression level between Arg7 and W14. Notably, Arg7 has a higher net photosynthesis rate in leaves, higher ribulose-1,5-bisphosphate carboxy-lase oxygenase activities in leaves, and higher AGPase activity in roots. This resource is the first EST collection from wild cassava and should be of value for gene discovery, genome annotation and studies of Manihot evolution.http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/1477362188366-1648564722.pdfManihot esculenta|expressed sequence tag|unigene|pathway|expression pattern |
| spellingShingle | Yang ZHANG,Xin CHEN,Haiyan WANG,Zhiqiang XIA,Peng LING,Wenquan WANG Annotation and validation of genes involved in photosynthesis and starch synthesis from a Manihot full-length cDNA library Frontiers of Agricultural Science and Engineering Manihot esculenta|expressed sequence tag|unigene|pathway|expression pattern |
| title | Annotation and validation of genes involved in photosynthesis and starch synthesis from a Manihot full-length cDNA library |
| title_full | Annotation and validation of genes involved in photosynthesis and starch synthesis from a Manihot full-length cDNA library |
| title_fullStr | Annotation and validation of genes involved in photosynthesis and starch synthesis from a Manihot full-length cDNA library |
| title_full_unstemmed | Annotation and validation of genes involved in photosynthesis and starch synthesis from a Manihot full-length cDNA library |
| title_short | Annotation and validation of genes involved in photosynthesis and starch synthesis from a Manihot full-length cDNA library |
| title_sort | annotation and validation of genes involved in photosynthesis and starch synthesis from a manihot full length cdna library |
| topic | Manihot esculenta|expressed sequence tag|unigene|pathway|expression pattern |
| url | http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/1477362188366-1648564722.pdf |
| work_keys_str_mv | AT yangzhangxinchenhaiyanwangzhiqiangxiapenglingwenquanwang annotationandvalidationofgenesinvolvedinphotosynthesisandstarchsynthesisfromamanihotfulllengthcdnalibrary |